Comparison of the Staphylococcal Chromosome Cassette (SCC) <i>mec</i> in Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Non-<i>aureus</i> Staphylococci (MRNAS) from Animals and Humans
Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and non-<i>aureus</i> staphylococci (MRNAS) cause different infections in animals, including mastitis, in livestock and humans. This study aimed to identify and compare the staphylococcal chromosome cassette <i>m...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/3/256 |
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| author | Cyrille Ngassam Tchamba Jean-Noël Duprez Pierrick Lucas Yannick Blanchard Filip Boyen Freddy Haesebrouck Maria A. Argudín Jacques Mainil Damien Thiry |
| author_facet | Cyrille Ngassam Tchamba Jean-Noël Duprez Pierrick Lucas Yannick Blanchard Filip Boyen Freddy Haesebrouck Maria A. Argudín Jacques Mainil Damien Thiry |
| author_sort | Cyrille Ngassam Tchamba |
| collection | DOAJ |
| description | Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and non-<i>aureus</i> staphylococci (MRNAS) cause different infections in animals, including mastitis, in livestock and humans. This study aimed to identify and compare the staphylococcal chromosome cassette <i>mec</i> (SCC<i>mec</i>) types of MRSA or MRNAS isolated from several animal species and humans in different countries. Of 1462 <i>S. aureus</i> and non-<i>aureus</i> staphylococci, 68 grew on Chrom MRSA ID<sup>®</sup> agar, were phenotypically resistant to cefoxitin and tested positive with the PCR for the <i>mecA</i> gene. These 60 MRSA and 8 MRNAS were isolated in Belgium mainly from cows (livestock-associated (LA) MRS) and humans (community-acquired (CA) MRS) and in Japan from dogs and cats. The SCC<i>mec</i> cassettes were identified by multiplex PCR in 52 MRSA and 7 MRNAS and by whole genome sequencing (WGS) in 8 additional MRSA. The SCC<i>mec</i> types IV and V were the most frequent in Belgian LA-MRS and CA-MRS, while the SCC<i>mec</i> type II was identified in four of the five Japanese MRSA. The remaining isolate was a bovine <i>S. haemolyticus</i> in which no SCC<i>mec</i> was identified. These results confirm the high prevalence of the SCC<i>mec</i> types IV and V in LA-MRS and CA-MRS in Belgium, emphasizing the possible public health hazard of the former, and the absence of SCC<i>mec</i> in some MRNAS. |
| first_indexed | 2024-03-09T05:37:50Z |
| format | Article |
| id | doaj.art-86d4efd637a841aca0411d3096725641 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T05:37:50Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-86d4efd637a841aca0411d30967256412023-12-03T12:26:58ZengMDPI AGAntibiotics2079-63822021-03-0110325610.3390/antibiotics10030256Comparison of the Staphylococcal Chromosome Cassette (SCC) <i>mec</i> in Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Non-<i>aureus</i> Staphylococci (MRNAS) from Animals and HumansCyrille Ngassam Tchamba0Jean-Noël Duprez1Pierrick Lucas2Yannick Blanchard3Filip Boyen4Freddy Haesebrouck5Maria A. Argudín6Jacques Mainil7Damien Thiry8Bacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animals and Health (FARAH), University of Liège, Quartier Vallée 2, Avenue de Cureghem 6, 4000 Liège, BelgiumBacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animals and Health (FARAH), University of Liège, Quartier Vallée 2, Avenue de Cureghem 6, 4000 Liège, BelgiumViral Genetics and Bio-Security Unit, ANSES, Ploufragan-Plouzané Laboratory, Rue des Fusillés, 22440 Ploufragan, FranceViral Genetics and Bio-Security Unit, ANSES, Ploufragan-Plouzané Laboratory, Rue des Fusillés, 22440 Ploufragan, FranceDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumLaboratory of Molecular Biology, Cliniques Universitaires Saint-Luc, Catholic University of Louvain, Avenue Hippocrate 10, 1200 Brussels, BelgiumBacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animals and Health (FARAH), University of Liège, Quartier Vallée 2, Avenue de Cureghem 6, 4000 Liège, BelgiumBacteriology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animals and Health (FARAH), University of Liège, Quartier Vallée 2, Avenue de Cureghem 6, 4000 Liège, BelgiumMethicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and non-<i>aureus</i> staphylococci (MRNAS) cause different infections in animals, including mastitis, in livestock and humans. This study aimed to identify and compare the staphylococcal chromosome cassette <i>mec</i> (SCC<i>mec</i>) types of MRSA or MRNAS isolated from several animal species and humans in different countries. Of 1462 <i>S. aureus</i> and non-<i>aureus</i> staphylococci, 68 grew on Chrom MRSA ID<sup>®</sup> agar, were phenotypically resistant to cefoxitin and tested positive with the PCR for the <i>mecA</i> gene. These 60 MRSA and 8 MRNAS were isolated in Belgium mainly from cows (livestock-associated (LA) MRS) and humans (community-acquired (CA) MRS) and in Japan from dogs and cats. The SCC<i>mec</i> cassettes were identified by multiplex PCR in 52 MRSA and 7 MRNAS and by whole genome sequencing (WGS) in 8 additional MRSA. The SCC<i>mec</i> types IV and V were the most frequent in Belgian LA-MRS and CA-MRS, while the SCC<i>mec</i> type II was identified in four of the five Japanese MRSA. The remaining isolate was a bovine <i>S. haemolyticus</i> in which no SCC<i>mec</i> was identified. These results confirm the high prevalence of the SCC<i>mec</i> types IV and V in LA-MRS and CA-MRS in Belgium, emphasizing the possible public health hazard of the former, and the absence of SCC<i>mec</i> in some MRNAS.https://www.mdpi.com/2079-6382/10/3/256<i>Staphylococcus aureus</i>non-<i>aureus</i> staphylococcimethicillin resistance<i>mecA</i> staphylococcal cassette chromosomecattlehumans |
| spellingShingle | Cyrille Ngassam Tchamba Jean-Noël Duprez Pierrick Lucas Yannick Blanchard Filip Boyen Freddy Haesebrouck Maria A. Argudín Jacques Mainil Damien Thiry Comparison of the Staphylococcal Chromosome Cassette (SCC) <i>mec</i> in Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Non-<i>aureus</i> Staphylococci (MRNAS) from Animals and Humans Antibiotics <i>Staphylococcus aureus</i> non-<i>aureus</i> staphylococci methicillin resistance <i>mecA</i> staphylococcal cassette chromosome cattle humans |
| title | Comparison of the Staphylococcal Chromosome Cassette (SCC) <i>mec</i> in Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Non-<i>aureus</i> Staphylococci (MRNAS) from Animals and Humans |
| title_full | Comparison of the Staphylococcal Chromosome Cassette (SCC) <i>mec</i> in Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Non-<i>aureus</i> Staphylococci (MRNAS) from Animals and Humans |
| title_fullStr | Comparison of the Staphylococcal Chromosome Cassette (SCC) <i>mec</i> in Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Non-<i>aureus</i> Staphylococci (MRNAS) from Animals and Humans |
| title_full_unstemmed | Comparison of the Staphylococcal Chromosome Cassette (SCC) <i>mec</i> in Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Non-<i>aureus</i> Staphylococci (MRNAS) from Animals and Humans |
| title_short | Comparison of the Staphylococcal Chromosome Cassette (SCC) <i>mec</i> in Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Non-<i>aureus</i> Staphylococci (MRNAS) from Animals and Humans |
| title_sort | comparison of the staphylococcal chromosome cassette scc i mec i in methicillin resistant i staphylococcus aureus i mrsa and non i aureus i staphylococci mrnas from animals and humans |
| topic | <i>Staphylococcus aureus</i> non-<i>aureus</i> staphylococci methicillin resistance <i>mecA</i> staphylococcal cassette chromosome cattle humans |
| url | https://www.mdpi.com/2079-6382/10/3/256 |
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