Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus
The C2H2-type zinc finger proteins (C2H2-ZFPs) regulate various developmental processes and abiotic stress responses in eukaryotes. Yet, a comprehensive analysis of these transcription factors which could be used to find candidate genes related to the control the development and abiotic stress toler...
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PeerJ Inc.
2022-01-01
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| Online Access: | https://peerj.com/articles/12654.pdf |
| _version_ | 1797425978441465856 |
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| author | Qiangqiang Ding Hongyuan Zhao Peilei Zhu Xiangting Jiang Fan Nie Guoqing Li |
| author_facet | Qiangqiang Ding Hongyuan Zhao Peilei Zhu Xiangting Jiang Fan Nie Guoqing Li |
| author_sort | Qiangqiang Ding |
| collection | DOAJ |
| description | The C2H2-type zinc finger proteins (C2H2-ZFPs) regulate various developmental processes and abiotic stress responses in eukaryotes. Yet, a comprehensive analysis of these transcription factors which could be used to find candidate genes related to the control the development and abiotic stress tolerance has not been performed in Pleurotus ostreatus. To fill this knowledge gap, 18 C2H2-ZFs were identified in the P. ostreatus genome. Phylogenetic analysis indicated that these proteins have dissimilar amino acid sequences. In addition, these proteins had variable protein characteristics, gene intron-exon structures, and motif compositions. The expression patterns of PoC2H2-ZFs in mycelia, primordia, and young and mature fruiting bodies were investigated using qRT-PCR. The expression of some PoC2H2-ZFs is regulated by auxin and cytokinin. Moreover, members of PoC2H2-ZFs expression levels are changed dramatically under heat and cold stress, suggesting that these genes may participate in abiotic stress responses. These findings could be used to study the role of P. ostreatus-derived C2H2-ZFs in development and stress tolerance. |
| first_indexed | 2024-03-09T08:23:45Z |
| format | Article |
| id | doaj.art-86d9b1f3472043b39ff76de50d2e47bd |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:23:45Z |
| publishDate | 2022-01-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-86d9b1f3472043b39ff76de50d2e47bd2023-12-02T21:37:43ZengPeerJ Inc.PeerJ2167-83592022-01-0110e1265410.7717/peerj.12654Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatusQiangqiang Ding0Hongyuan Zhao1Peilei Zhu2Xiangting Jiang3Fan Nie4Guoqing Li5Institute of Horticulture, Anhui Academy of Agricultural Sciences, Heifei, Anhui Province, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Heifei, Anhui Province, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Heifei, Anhui Province, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Heifei, Anhui Province, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Heifei, Anhui Province, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Heifei, Anhui Province, ChinaThe C2H2-type zinc finger proteins (C2H2-ZFPs) regulate various developmental processes and abiotic stress responses in eukaryotes. Yet, a comprehensive analysis of these transcription factors which could be used to find candidate genes related to the control the development and abiotic stress tolerance has not been performed in Pleurotus ostreatus. To fill this knowledge gap, 18 C2H2-ZFs were identified in the P. ostreatus genome. Phylogenetic analysis indicated that these proteins have dissimilar amino acid sequences. In addition, these proteins had variable protein characteristics, gene intron-exon structures, and motif compositions. The expression patterns of PoC2H2-ZFs in mycelia, primordia, and young and mature fruiting bodies were investigated using qRT-PCR. The expression of some PoC2H2-ZFs is regulated by auxin and cytokinin. Moreover, members of PoC2H2-ZFs expression levels are changed dramatically under heat and cold stress, suggesting that these genes may participate in abiotic stress responses. These findings could be used to study the role of P. ostreatus-derived C2H2-ZFs in development and stress tolerance.https://peerj.com/articles/12654.pdfPleurotus ostreatusC2H2-ZFPsExpression patternsHormone responseAbiotic stress |
| spellingShingle | Qiangqiang Ding Hongyuan Zhao Peilei Zhu Xiangting Jiang Fan Nie Guoqing Li Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus PeerJ Pleurotus ostreatus C2H2-ZFPs Expression patterns Hormone response Abiotic stress |
| title | Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
| title_full | Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
| title_fullStr | Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
| title_full_unstemmed | Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
| title_short | Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
| title_sort | genome wide identification and expression analyses of c2h2 zinc finger transcription factors in pleurotus ostreatus |
| topic | Pleurotus ostreatus C2H2-ZFPs Expression patterns Hormone response Abiotic stress |
| url | https://peerj.com/articles/12654.pdf |
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