Evaluation of an Antibiotic Susceptibility Testing Method on <i>Enterobacterales</i>-Positive Blood Cultures in Less Than 8 h Using the Rapid Mueller-Hinton Diffusion Method in Conjunction with the SIRscan 2000 Automatic Reading Device
<i>Enterobacterales</i> bloodstream infections are life-threatening and require rapid, targeted antibiotherapy based on antibiotic susceptibility testing (AST). A new method using Muller-Hinton Rapid-SIR (MHR-SIR) agar (i2a, Montpellier, France) allows complete direct AST (dAST) to be re...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/7/1377 |
| _version_ | 1797416876092948480 |
|---|---|
| author | Mathilde Payen Alice Gaudart Kevin Legueult James Kasprzak Audrey Emery Grégoire Mutambayi Christian Pradier Frédéric Robin Romain Lotte Raymond Ruimy |
| author_facet | Mathilde Payen Alice Gaudart Kevin Legueult James Kasprzak Audrey Emery Grégoire Mutambayi Christian Pradier Frédéric Robin Romain Lotte Raymond Ruimy |
| author_sort | Mathilde Payen |
| collection | DOAJ |
| description | <i>Enterobacterales</i> bloodstream infections are life-threatening and require rapid, targeted antibiotherapy based on antibiotic susceptibility testing (AST). A new method using Muller-Hinton Rapid-SIR (MHR-SIR) agar (i2a, Montpellier, France) allows complete direct AST (dAST) to be read from positive blood culture bottles (BCBs) for all <i>Enterobacterales</i> species after 6–8 h of incubation. We evaluated (i) the performance of dAST from positive BCBs on MHR-SIR agar using two different inoculum protocols; (ii) the categorical agreement between dAST results obtained with MHR-SIR agar vs. those obtained with Muller-Hinton (MH) agar; and (iii) the ability of the MHR-SIR medium to detect β-lactam resistant <i>Enterobacterales.</i> Finally, we estimated the saved turnaround time (TAT) with MHR-SIR compared with MH agar in our 24/7 laboratory. Our results showed that the most suitable inoculation protocol for dAST on MHR-SIR agar was 1 drop of BCB/5 mL H<sub>2</sub>O. For monomicrobial <i>Enterobacterales</i> BCBs, dAST performed on MHR-SIR medium showed 99.3% categorical agreement with AST on MH agar. Furthermore, MHR-SIR agar allows early detection of β-lactam resistance mechanisms, including AmpC hyperproduction, extended-spectrum β-lactamase, and carbapenemase. Finally, TAT reduction in our 24/7 laboratory was 16 h, enabling a significantly faster provision of antibiotic advice. |
| first_indexed | 2024-03-09T06:10:32Z |
| format | Article |
| id | doaj.art-86dabe828b004809a54766a0832f6cca |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:10:32Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-86dabe828b004809a54766a0832f6cca2023-12-03T11:59:06ZengMDPI AGMicroorganisms2076-26072022-07-01107137710.3390/microorganisms10071377Evaluation of an Antibiotic Susceptibility Testing Method on <i>Enterobacterales</i>-Positive Blood Cultures in Less Than 8 h Using the Rapid Mueller-Hinton Diffusion Method in Conjunction with the SIRscan 2000 Automatic Reading DeviceMathilde Payen0Alice Gaudart1Kevin Legueult2James Kasprzak3Audrey Emery4Grégoire Mutambayi5Christian Pradier6Frédéric Robin7Romain Lotte8Raymond Ruimy9Laboratoire de Bactériologie, Hôpital L’Archet 2, Centre Hospitalier Universitaire de Nice, 06000 Nice, FranceLaboratoire de Bactériologie, Hôpital L’Archet 2, Centre Hospitalier Universitaire de Nice, 06000 Nice, FranceDépartement de Santé Publique, UR2CA, Université Côte d’Azur, Centre Hospitalier Universitaire de Nice, 06000 Nice, FranceLaboratoire de Bactériologie, Hôpital L’Archet 2, Centre Hospitalier Universitaire de Nice, 06000 Nice, FranceLaboratoire de Bactériologie, Hôpital L’Archet 2, Centre Hospitalier Universitaire de Nice, 06000 Nice, FranceLaboratoire de Bactériologie, Hôpital L’Archet 2, Centre Hospitalier Universitaire de Nice, 06000 Nice, FranceDépartement de Santé Publique, UR2CA, Université Côte d’Azur, Centre Hospitalier Universitaire de Nice, 06000 Nice, FranceLaboratoire de Bactériologie Clinique, Institut National de la Santé et de la Recherche Médicale U1071, INRA USC2018, Centre Hospitalier Universitaire de Clermont-Ferrand, Université Clermont Auvergne, 63000 Clermont-Ferrand, FranceLaboratoire de Bactériologie, Hôpital L’Archet 2, Centre Hospitalier Universitaire de Nice, 06000 Nice, FranceLaboratoire de Bactériologie, Hôpital L’Archet 2, Centre Hospitalier Universitaire de Nice, 06000 Nice, France<i>Enterobacterales</i> bloodstream infections are life-threatening and require rapid, targeted antibiotherapy based on antibiotic susceptibility testing (AST). A new method using Muller-Hinton Rapid-SIR (MHR-SIR) agar (i2a, Montpellier, France) allows complete direct AST (dAST) to be read from positive blood culture bottles (BCBs) for all <i>Enterobacterales</i> species after 6–8 h of incubation. We evaluated (i) the performance of dAST from positive BCBs on MHR-SIR agar using two different inoculum protocols; (ii) the categorical agreement between dAST results obtained with MHR-SIR agar vs. those obtained with Muller-Hinton (MH) agar; and (iii) the ability of the MHR-SIR medium to detect β-lactam resistant <i>Enterobacterales.</i> Finally, we estimated the saved turnaround time (TAT) with MHR-SIR compared with MH agar in our 24/7 laboratory. Our results showed that the most suitable inoculation protocol for dAST on MHR-SIR agar was 1 drop of BCB/5 mL H<sub>2</sub>O. For monomicrobial <i>Enterobacterales</i> BCBs, dAST performed on MHR-SIR medium showed 99.3% categorical agreement with AST on MH agar. Furthermore, MHR-SIR agar allows early detection of β-lactam resistance mechanisms, including AmpC hyperproduction, extended-spectrum β-lactamase, and carbapenemase. Finally, TAT reduction in our 24/7 laboratory was 16 h, enabling a significantly faster provision of antibiotic advice.https://www.mdpi.com/2076-2607/10/7/1377antibiotic susceptibility testingrapid ASTbacteremia |
| spellingShingle | Mathilde Payen Alice Gaudart Kevin Legueult James Kasprzak Audrey Emery Grégoire Mutambayi Christian Pradier Frédéric Robin Romain Lotte Raymond Ruimy Evaluation of an Antibiotic Susceptibility Testing Method on <i>Enterobacterales</i>-Positive Blood Cultures in Less Than 8 h Using the Rapid Mueller-Hinton Diffusion Method in Conjunction with the SIRscan 2000 Automatic Reading Device Microorganisms antibiotic susceptibility testing rapid AST bacteremia |
| title | Evaluation of an Antibiotic Susceptibility Testing Method on <i>Enterobacterales</i>-Positive Blood Cultures in Less Than 8 h Using the Rapid Mueller-Hinton Diffusion Method in Conjunction with the SIRscan 2000 Automatic Reading Device |
| title_full | Evaluation of an Antibiotic Susceptibility Testing Method on <i>Enterobacterales</i>-Positive Blood Cultures in Less Than 8 h Using the Rapid Mueller-Hinton Diffusion Method in Conjunction with the SIRscan 2000 Automatic Reading Device |
| title_fullStr | Evaluation of an Antibiotic Susceptibility Testing Method on <i>Enterobacterales</i>-Positive Blood Cultures in Less Than 8 h Using the Rapid Mueller-Hinton Diffusion Method in Conjunction with the SIRscan 2000 Automatic Reading Device |
| title_full_unstemmed | Evaluation of an Antibiotic Susceptibility Testing Method on <i>Enterobacterales</i>-Positive Blood Cultures in Less Than 8 h Using the Rapid Mueller-Hinton Diffusion Method in Conjunction with the SIRscan 2000 Automatic Reading Device |
| title_short | Evaluation of an Antibiotic Susceptibility Testing Method on <i>Enterobacterales</i>-Positive Blood Cultures in Less Than 8 h Using the Rapid Mueller-Hinton Diffusion Method in Conjunction with the SIRscan 2000 Automatic Reading Device |
| title_sort | evaluation of an antibiotic susceptibility testing method on i enterobacterales i positive blood cultures in less than 8 h using the rapid mueller hinton diffusion method in conjunction with the sirscan 2000 automatic reading device |
| topic | antibiotic susceptibility testing rapid AST bacteremia |
| url | https://www.mdpi.com/2076-2607/10/7/1377 |
| work_keys_str_mv | AT mathildepayen evaluationofanantibioticsusceptibilitytestingmethodonienterobacteralesipositivebloodculturesinlessthan8husingtherapidmuellerhintondiffusionmethodinconjunctionwiththesirscan2000automaticreadingdevice AT alicegaudart evaluationofanantibioticsusceptibilitytestingmethodonienterobacteralesipositivebloodculturesinlessthan8husingtherapidmuellerhintondiffusionmethodinconjunctionwiththesirscan2000automaticreadingdevice AT kevinlegueult evaluationofanantibioticsusceptibilitytestingmethodonienterobacteralesipositivebloodculturesinlessthan8husingtherapidmuellerhintondiffusionmethodinconjunctionwiththesirscan2000automaticreadingdevice AT jameskasprzak evaluationofanantibioticsusceptibilitytestingmethodonienterobacteralesipositivebloodculturesinlessthan8husingtherapidmuellerhintondiffusionmethodinconjunctionwiththesirscan2000automaticreadingdevice AT audreyemery evaluationofanantibioticsusceptibilitytestingmethodonienterobacteralesipositivebloodculturesinlessthan8husingtherapidmuellerhintondiffusionmethodinconjunctionwiththesirscan2000automaticreadingdevice AT gregoiremutambayi evaluationofanantibioticsusceptibilitytestingmethodonienterobacteralesipositivebloodculturesinlessthan8husingtherapidmuellerhintondiffusionmethodinconjunctionwiththesirscan2000automaticreadingdevice AT christianpradier evaluationofanantibioticsusceptibilitytestingmethodonienterobacteralesipositivebloodculturesinlessthan8husingtherapidmuellerhintondiffusionmethodinconjunctionwiththesirscan2000automaticreadingdevice AT fredericrobin evaluationofanantibioticsusceptibilitytestingmethodonienterobacteralesipositivebloodculturesinlessthan8husingtherapidmuellerhintondiffusionmethodinconjunctionwiththesirscan2000automaticreadingdevice AT romainlotte evaluationofanantibioticsusceptibilitytestingmethodonienterobacteralesipositivebloodculturesinlessthan8husingtherapidmuellerhintondiffusionmethodinconjunctionwiththesirscan2000automaticreadingdevice AT raymondruimy evaluationofanantibioticsusceptibilitytestingmethodonienterobacteralesipositivebloodculturesinlessthan8husingtherapidmuellerhintondiffusionmethodinconjunctionwiththesirscan2000automaticreadingdevice |