A Hydrogel as a Bespoke Delivery Platform for Stromal Cell-Derived Factor-1
The defined self-assembly of peptides (SAPs) into nanostructured bioactive hydrogels has great potential for repairing traumatic brain injuries, as they maintain a stable, homeostatic environment at an injury site, preventing further degeneration. They also present a bespoke platform to restore func...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Gels |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2310-2861/8/4/224 |
| _version_ | 1797434714030604288 |
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| author | Yi Wang Vanessa Penna Richard J. Williams Clare L. Parish David R. Nisbet |
| author_facet | Yi Wang Vanessa Penna Richard J. Williams Clare L. Parish David R. Nisbet |
| author_sort | Yi Wang |
| collection | DOAJ |
| description | The defined self-assembly of peptides (SAPs) into nanostructured bioactive hydrogels has great potential for repairing traumatic brain injuries, as they maintain a stable, homeostatic environment at an injury site, preventing further degeneration. They also present a bespoke platform to restore function via the naturalistic presentation of therapeutic proteins, such as stromal-cell-derived factor 1 (SDF-1), expressed by meningeal cells. A key challenge to the use of the SDF protein, however, is its rapid diffusion and degradation. Here, we engineered a homeostatic hydrogel produced by incorporating recombinant SDF-1 protein within a self-assembled peptide hydrogel to create a supportive milieu for transplanted cells. Our hydrogel can concomitantly deliver viable primary neural progenitor cells and sustained active SDF-1 to support the nascent graft, resulting in increased neuronal differentiation. Moreover, this homeostatic hydrogel can ensure a healthy and larger graft core without impeding neuronal fiber growth and innervation. These findings demonstrate the regenerative potential of these hydrogels to improve the integration of grafted cells to treat neural injuries and diseases. |
| first_indexed | 2024-03-09T10:36:20Z |
| format | Article |
| id | doaj.art-86e0405c5d624dc891450c23b7f82385 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T10:36:20Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-86e0405c5d624dc891450c23b7f823852023-12-01T20:55:59ZengMDPI AGGels2310-28612022-04-018422410.3390/gels8040224A Hydrogel as a Bespoke Delivery Platform for Stromal Cell-Derived Factor-1Yi Wang0Vanessa Penna1Richard J. Williams2Clare L. Parish3David R. Nisbet4The Graeme Clark Institute, The University of Melbourne, Melbourne 3010, AustraliaThe Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne 3052, AustraliaInstitute for Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University, Melbourne 3216, AustraliaThe Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Melbourne 3052, AustraliaThe Graeme Clark Institute, The University of Melbourne, Melbourne 3010, AustraliaThe defined self-assembly of peptides (SAPs) into nanostructured bioactive hydrogels has great potential for repairing traumatic brain injuries, as they maintain a stable, homeostatic environment at an injury site, preventing further degeneration. They also present a bespoke platform to restore function via the naturalistic presentation of therapeutic proteins, such as stromal-cell-derived factor 1 (SDF-1), expressed by meningeal cells. A key challenge to the use of the SDF protein, however, is its rapid diffusion and degradation. Here, we engineered a homeostatic hydrogel produced by incorporating recombinant SDF-1 protein within a self-assembled peptide hydrogel to create a supportive milieu for transplanted cells. Our hydrogel can concomitantly deliver viable primary neural progenitor cells and sustained active SDF-1 to support the nascent graft, resulting in increased neuronal differentiation. Moreover, this homeostatic hydrogel can ensure a healthy and larger graft core without impeding neuronal fiber growth and innervation. These findings demonstrate the regenerative potential of these hydrogels to improve the integration of grafted cells to treat neural injuries and diseases.https://www.mdpi.com/2310-2861/8/4/224cell transplantationself-assembly peptide (SAP)hydrogellamininstromal-cell-derived factor (SDF)cell survival |
| spellingShingle | Yi Wang Vanessa Penna Richard J. Williams Clare L. Parish David R. Nisbet A Hydrogel as a Bespoke Delivery Platform for Stromal Cell-Derived Factor-1 Gels cell transplantation self-assembly peptide (SAP) hydrogel laminin stromal-cell-derived factor (SDF) cell survival |
| title | A Hydrogel as a Bespoke Delivery Platform for Stromal Cell-Derived Factor-1 |
| title_full | A Hydrogel as a Bespoke Delivery Platform for Stromal Cell-Derived Factor-1 |
| title_fullStr | A Hydrogel as a Bespoke Delivery Platform for Stromal Cell-Derived Factor-1 |
| title_full_unstemmed | A Hydrogel as a Bespoke Delivery Platform for Stromal Cell-Derived Factor-1 |
| title_short | A Hydrogel as a Bespoke Delivery Platform for Stromal Cell-Derived Factor-1 |
| title_sort | hydrogel as a bespoke delivery platform for stromal cell derived factor 1 |
| topic | cell transplantation self-assembly peptide (SAP) hydrogel laminin stromal-cell-derived factor (SDF) cell survival |
| url | https://www.mdpi.com/2310-2861/8/4/224 |
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