Research Note: Delay in sampling influences the profile of phytate in gizzard digesta and ileal digestibility of phosphorus in broilers
The objective of this study was to investigate the effect of different durations of time delay when sampling digesta from the gizzard and ileum of broilers on the degradation of myo-inositol hexakisphosphate (InsP6) and digestibility of phosphorus (P). There was 1 experimental diet with a supplement...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579120304144 |
| _version_ | 1818512539362787328 |
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| author | H.X. Zhai A.J. Cowieson M. Lehmann J.W. Wilson |
| author_facet | H.X. Zhai A.J. Cowieson M. Lehmann J.W. Wilson |
| author_sort | H.X. Zhai |
| collection | DOAJ |
| description | The objective of this study was to investigate the effect of different durations of time delay when sampling digesta from the gizzard and ileum of broilers on the degradation of myo-inositol hexakisphosphate (InsP6) and digestibility of phosphorus (P). There was 1 experimental diet with a supplemental phytase activity of 1,212 phytase units/kg feed, which was provided to birds from day 13 to 18 after hatching. The diet was formulated to provide 6.6 g/kg Ca and 1.9 g/kg nonphytate P and fed to 24 cages of 6 birds. The 24 cages of birds were further randomly divided into 6 subgroups of 4 cages from which the digesta samples in the gizzard and ileum were collected at 0, 5, 10, or 20 min postmortem. The results showed that the concentration of InsP6 decreased linearly (P = 0.002), InsP5 decreased quadratically (P = 0.038), and the summation of concentrations of P in InsP6–4 decreased linearly (P = 0.028) in the gizzard digesta with the increasing delay of sampling. In the ileum, the digestibility of phytate P tended to decrease linearly (P = 0.087), and the digestibility of total P decreased linearly (P = 0.026) with prolonged delay. In conclusion, delay in sampling could alter the measured profile of InsP esters in gizzard digesta probably because of a continued effect of supplemental phytase, while the ileal digestibility of total P could diminish. Therefore, standard sampling procedures should be implemented to minimize variance. |
| first_indexed | 2024-12-10T23:48:17Z |
| format | Article |
| id | doaj.art-86e0942b89154dbb903268020f15393d |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-10T23:48:17Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-86e0942b89154dbb903268020f15393d2022-12-22T01:28:51ZengElsevierPoultry Science0032-57912020-10-01991050655069Research Note: Delay in sampling influences the profile of phytate in gizzard digesta and ileal digestibility of phosphorus in broilersH.X. Zhai0A.J. Cowieson1M. Lehmann2J.W. Wilson3DSM (China) Animal Nutrition Research Center Co., Ltd, Bazhou, P. R. China; Corresponding author:DSM Nutritional Products Ltd, Kaiseraugst, SwitzerlandDSM Nutritional Products Ltd, Kaiseraugst, SwitzerlandDSM Nutritional Products Ltd, Kaiseraugst, SwitzerlandThe objective of this study was to investigate the effect of different durations of time delay when sampling digesta from the gizzard and ileum of broilers on the degradation of myo-inositol hexakisphosphate (InsP6) and digestibility of phosphorus (P). There was 1 experimental diet with a supplemental phytase activity of 1,212 phytase units/kg feed, which was provided to birds from day 13 to 18 after hatching. The diet was formulated to provide 6.6 g/kg Ca and 1.9 g/kg nonphytate P and fed to 24 cages of 6 birds. The 24 cages of birds were further randomly divided into 6 subgroups of 4 cages from which the digesta samples in the gizzard and ileum were collected at 0, 5, 10, or 20 min postmortem. The results showed that the concentration of InsP6 decreased linearly (P = 0.002), InsP5 decreased quadratically (P = 0.038), and the summation of concentrations of P in InsP6–4 decreased linearly (P = 0.028) in the gizzard digesta with the increasing delay of sampling. In the ileum, the digestibility of phytate P tended to decrease linearly (P = 0.087), and the digestibility of total P decreased linearly (P = 0.026) with prolonged delay. In conclusion, delay in sampling could alter the measured profile of InsP esters in gizzard digesta probably because of a continued effect of supplemental phytase, while the ileal digestibility of total P could diminish. Therefore, standard sampling procedures should be implemented to minimize variance.http://www.sciencedirect.com/science/article/pii/S0032579120304144broilerdigestibilityphosphorusphytasephytatesampling |
| spellingShingle | H.X. Zhai A.J. Cowieson M. Lehmann J.W. Wilson Research Note: Delay in sampling influences the profile of phytate in gizzard digesta and ileal digestibility of phosphorus in broilers Poultry Science broiler digestibility phosphorus phytase phytate sampling |
| title | Research Note: Delay in sampling influences the profile of phytate in gizzard digesta and ileal digestibility of phosphorus in broilers |
| title_full | Research Note: Delay in sampling influences the profile of phytate in gizzard digesta and ileal digestibility of phosphorus in broilers |
| title_fullStr | Research Note: Delay in sampling influences the profile of phytate in gizzard digesta and ileal digestibility of phosphorus in broilers |
| title_full_unstemmed | Research Note: Delay in sampling influences the profile of phytate in gizzard digesta and ileal digestibility of phosphorus in broilers |
| title_short | Research Note: Delay in sampling influences the profile of phytate in gizzard digesta and ileal digestibility of phosphorus in broilers |
| title_sort | research note delay in sampling influences the profile of phytate in gizzard digesta and ileal digestibility of phosphorus in broilers |
| topic | broiler digestibility phosphorus phytase phytate sampling |
| url | http://www.sciencedirect.com/science/article/pii/S0032579120304144 |
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