Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures

In this work we developed a bi-functional Bacterial-Nano-Cellulose (BNC) carrier system for cell cultures of <i>Chelidonium majus</i>—a medicinal plant producing antimicrobial compounds. The porous BNC was biosynthesized for 3, 5 or 7 days by the non-pathogenic <i>Komagataeibacter...

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Main Authors: Sylwia Zielińska, Adam Matkowski, Karolina Dydak, Monika Ewa Czerwińska, Magdalena Dziągwa-Becker, Mariusz Kucharski, Magdalena Wójciak, Ireneusz Sowa, Stanisława Plińska, Karol Fijałkowski, Daria Ciecholewska-Juśko, Michał Broda, Damian Gorczyca, Adam Junka
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Materials
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Online Access:https://www.mdpi.com/1996-1944/15/1/16
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author Sylwia Zielińska
Adam Matkowski
Karolina Dydak
Monika Ewa Czerwińska
Magdalena Dziągwa-Becker
Mariusz Kucharski
Magdalena Wójciak
Ireneusz Sowa
Stanisława Plińska
Karol Fijałkowski
Daria Ciecholewska-Juśko
Michał Broda
Damian Gorczyca
Adam Junka
author_facet Sylwia Zielińska
Adam Matkowski
Karolina Dydak
Monika Ewa Czerwińska
Magdalena Dziągwa-Becker
Mariusz Kucharski
Magdalena Wójciak
Ireneusz Sowa
Stanisława Plińska
Karol Fijałkowski
Daria Ciecholewska-Juśko
Michał Broda
Damian Gorczyca
Adam Junka
author_sort Sylwia Zielińska
collection DOAJ
description In this work we developed a bi-functional Bacterial-Nano-Cellulose (BNC) carrier system for cell cultures of <i>Chelidonium majus</i>—a medicinal plant producing antimicrobial compounds. The porous BNC was biosynthesized for 3, 5 or 7 days by the non-pathogenic <i>Komagataeibacter xylinus</i> bacteria and used in three forms: (1) Without removal of <i>K. xylinus</i> cells, (2) partially cleaned up from the remaining <i>K. xylinus</i> cells using water washing and (3) fully purified with NaOH leaving no bacterial cells remains. The suspended <i>C. majus</i> cells were inoculated on the BNC pieces in liquid medium and the functionalized BNC was harvested and subjected to scanning electron microscopy observation and analyzed for the content of <i>C. majus</i> metabolites as well as to antimicrobial assays and tested for potential proinflammatory irritating activity in human neutrophils. The highest content and the most complex composition of pharmacologically active substances was found in 3-day-old, unpurified BNC, which was tested for its bioactivity. The assays based on the IL-1β, IL-8 and TNF-α secretion in an in vitro model showed an anti-inflammatory effect of this particular biomatrix. Moreover, 3-day-old-BNC displayed antimicrobial and antibiofilm activity against <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i> and <i>Candida albicans</i>. The results of the research indicated a possible application of such modified composites, against microbial pathogens, especially in local surface infections, where plant metabolite-enriched BNC may be used as the occlusive dressing.
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spelling doaj.art-86e2123ac5544ef2aab0e22222b24dda2023-11-23T11:46:48ZengMDPI AGMaterials1996-19442021-12-011511610.3390/ma15010016Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell CulturesSylwia Zielińska0Adam Matkowski1Karolina Dydak2Monika Ewa Czerwińska3Magdalena Dziągwa-Becker4Mariusz Kucharski5Magdalena Wójciak6Ireneusz Sowa7Stanisława Plińska8Karol Fijałkowski9Daria Ciecholewska-Juśko10Michał Broda11Damian Gorczyca12Adam Junka13Department of Pharmaceutical Biology and Biotechnology, Division of Pharmaceutical Biotechnology, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Pharmaceutical Biology and Biotechnology, Division of Pharmaceutical Biology and Botany, Wroclaw Medical University, 50-556 Wroclaw, PolandPharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Biochemistry and Pharmacogenomics, Faculty of Pharmacy, Medical University of Warsaw, 02-097 Warszawa, PolandDepartment of Weed Science and Tillage Systems, Institute of Soil Science and Plant Cultivation State Research Institute, 50-540 Wrocław, PolandDepartment of Weed Science and Tillage Systems, Institute of Soil Science and Plant Cultivation State Research Institute, 50-540 Wrocław, PolandDepartment of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Inorganic Chemistry, Wroclaw Medical University, 50-556 Wrocław, PolandDepartment of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, 70-311 Szczecin, PolandDepartment of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, 70-311 Szczecin, PolandDepartment of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, 70-311 Szczecin, PolandFaculty of Medicine, Lazarski University, 02-662 Warszawa, PolandPharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-556 Wroclaw, PolandIn this work we developed a bi-functional Bacterial-Nano-Cellulose (BNC) carrier system for cell cultures of <i>Chelidonium majus</i>—a medicinal plant producing antimicrobial compounds. The porous BNC was biosynthesized for 3, 5 or 7 days by the non-pathogenic <i>Komagataeibacter xylinus</i> bacteria and used in three forms: (1) Without removal of <i>K. xylinus</i> cells, (2) partially cleaned up from the remaining <i>K. xylinus</i> cells using water washing and (3) fully purified with NaOH leaving no bacterial cells remains. The suspended <i>C. majus</i> cells were inoculated on the BNC pieces in liquid medium and the functionalized BNC was harvested and subjected to scanning electron microscopy observation and analyzed for the content of <i>C. majus</i> metabolites as well as to antimicrobial assays and tested for potential proinflammatory irritating activity in human neutrophils. The highest content and the most complex composition of pharmacologically active substances was found in 3-day-old, unpurified BNC, which was tested for its bioactivity. The assays based on the IL-1β, IL-8 and TNF-α secretion in an in vitro model showed an anti-inflammatory effect of this particular biomatrix. Moreover, 3-day-old-BNC displayed antimicrobial and antibiofilm activity against <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i> and <i>Candida albicans</i>. The results of the research indicated a possible application of such modified composites, against microbial pathogens, especially in local surface infections, where plant metabolite-enriched BNC may be used as the occlusive dressing.https://www.mdpi.com/1996-1944/15/1/16bacterial nanocellulose<i>Chelidonium majus</i>cell culture carrierphenolic compoundsisoquinoline alkaloidsmicrobial pathogens
spellingShingle Sylwia Zielińska
Adam Matkowski
Karolina Dydak
Monika Ewa Czerwińska
Magdalena Dziągwa-Becker
Mariusz Kucharski
Magdalena Wójciak
Ireneusz Sowa
Stanisława Plińska
Karol Fijałkowski
Daria Ciecholewska-Juśko
Michał Broda
Damian Gorczyca
Adam Junka
Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures
Materials
bacterial nanocellulose
<i>Chelidonium majus</i>
cell culture carrier
phenolic compounds
isoquinoline alkaloids
microbial pathogens
title Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures
title_full Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures
title_fullStr Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures
title_full_unstemmed Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures
title_short Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures
title_sort bacterial nanocellulose fortified with antimicrobial and anti inflammatory natural products from i chelidonium majus i plant cell cultures
topic bacterial nanocellulose
<i>Chelidonium majus</i>
cell culture carrier
phenolic compounds
isoquinoline alkaloids
microbial pathogens
url https://www.mdpi.com/1996-1944/15/1/16
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