Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures
In this work we developed a bi-functional Bacterial-Nano-Cellulose (BNC) carrier system for cell cultures of <i>Chelidonium majus</i>—a medicinal plant producing antimicrobial compounds. The porous BNC was biosynthesized for 3, 5 or 7 days by the non-pathogenic <i>Komagataeibacter...
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author | Sylwia Zielińska Adam Matkowski Karolina Dydak Monika Ewa Czerwińska Magdalena Dziągwa-Becker Mariusz Kucharski Magdalena Wójciak Ireneusz Sowa Stanisława Plińska Karol Fijałkowski Daria Ciecholewska-Juśko Michał Broda Damian Gorczyca Adam Junka |
author_facet | Sylwia Zielińska Adam Matkowski Karolina Dydak Monika Ewa Czerwińska Magdalena Dziągwa-Becker Mariusz Kucharski Magdalena Wójciak Ireneusz Sowa Stanisława Plińska Karol Fijałkowski Daria Ciecholewska-Juśko Michał Broda Damian Gorczyca Adam Junka |
author_sort | Sylwia Zielińska |
collection | DOAJ |
description | In this work we developed a bi-functional Bacterial-Nano-Cellulose (BNC) carrier system for cell cultures of <i>Chelidonium majus</i>—a medicinal plant producing antimicrobial compounds. The porous BNC was biosynthesized for 3, 5 or 7 days by the non-pathogenic <i>Komagataeibacter xylinus</i> bacteria and used in three forms: (1) Without removal of <i>K. xylinus</i> cells, (2) partially cleaned up from the remaining <i>K. xylinus</i> cells using water washing and (3) fully purified with NaOH leaving no bacterial cells remains. The suspended <i>C. majus</i> cells were inoculated on the BNC pieces in liquid medium and the functionalized BNC was harvested and subjected to scanning electron microscopy observation and analyzed for the content of <i>C. majus</i> metabolites as well as to antimicrobial assays and tested for potential proinflammatory irritating activity in human neutrophils. The highest content and the most complex composition of pharmacologically active substances was found in 3-day-old, unpurified BNC, which was tested for its bioactivity. The assays based on the IL-1β, IL-8 and TNF-α secretion in an in vitro model showed an anti-inflammatory effect of this particular biomatrix. Moreover, 3-day-old-BNC displayed antimicrobial and antibiofilm activity against <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i> and <i>Candida albicans</i>. The results of the research indicated a possible application of such modified composites, against microbial pathogens, especially in local surface infections, where plant metabolite-enriched BNC may be used as the occlusive dressing. |
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id | doaj.art-86e2123ac5544ef2aab0e22222b24dda |
institution | Directory Open Access Journal |
issn | 1996-1944 |
language | English |
last_indexed | 2024-03-10T03:34:51Z |
publishDate | 2021-12-01 |
publisher | MDPI AG |
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spelling | doaj.art-86e2123ac5544ef2aab0e22222b24dda2023-11-23T11:46:48ZengMDPI AGMaterials1996-19442021-12-011511610.3390/ma15010016Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell CulturesSylwia Zielińska0Adam Matkowski1Karolina Dydak2Monika Ewa Czerwińska3Magdalena Dziągwa-Becker4Mariusz Kucharski5Magdalena Wójciak6Ireneusz Sowa7Stanisława Plińska8Karol Fijałkowski9Daria Ciecholewska-Juśko10Michał Broda11Damian Gorczyca12Adam Junka13Department of Pharmaceutical Biology and Biotechnology, Division of Pharmaceutical Biotechnology, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Pharmaceutical Biology and Biotechnology, Division of Pharmaceutical Biology and Botany, Wroclaw Medical University, 50-556 Wroclaw, PolandPharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Biochemistry and Pharmacogenomics, Faculty of Pharmacy, Medical University of Warsaw, 02-097 Warszawa, PolandDepartment of Weed Science and Tillage Systems, Institute of Soil Science and Plant Cultivation State Research Institute, 50-540 Wrocław, PolandDepartment of Weed Science and Tillage Systems, Institute of Soil Science and Plant Cultivation State Research Institute, 50-540 Wrocław, PolandDepartment of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Inorganic Chemistry, Wroclaw Medical University, 50-556 Wrocław, PolandDepartment of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, 70-311 Szczecin, PolandDepartment of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, 70-311 Szczecin, PolandDepartment of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, 70-311 Szczecin, PolandFaculty of Medicine, Lazarski University, 02-662 Warszawa, PolandPharmaceutical Microbiology and Parasitology, Wroclaw Medical University, 50-556 Wroclaw, PolandIn this work we developed a bi-functional Bacterial-Nano-Cellulose (BNC) carrier system for cell cultures of <i>Chelidonium majus</i>—a medicinal plant producing antimicrobial compounds. The porous BNC was biosynthesized for 3, 5 or 7 days by the non-pathogenic <i>Komagataeibacter xylinus</i> bacteria and used in three forms: (1) Without removal of <i>K. xylinus</i> cells, (2) partially cleaned up from the remaining <i>K. xylinus</i> cells using water washing and (3) fully purified with NaOH leaving no bacterial cells remains. The suspended <i>C. majus</i> cells were inoculated on the BNC pieces in liquid medium and the functionalized BNC was harvested and subjected to scanning electron microscopy observation and analyzed for the content of <i>C. majus</i> metabolites as well as to antimicrobial assays and tested for potential proinflammatory irritating activity in human neutrophils. The highest content and the most complex composition of pharmacologically active substances was found in 3-day-old, unpurified BNC, which was tested for its bioactivity. The assays based on the IL-1β, IL-8 and TNF-α secretion in an in vitro model showed an anti-inflammatory effect of this particular biomatrix. Moreover, 3-day-old-BNC displayed antimicrobial and antibiofilm activity against <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i> and <i>Candida albicans</i>. The results of the research indicated a possible application of such modified composites, against microbial pathogens, especially in local surface infections, where plant metabolite-enriched BNC may be used as the occlusive dressing.https://www.mdpi.com/1996-1944/15/1/16bacterial nanocellulose<i>Chelidonium majus</i>cell culture carrierphenolic compoundsisoquinoline alkaloidsmicrobial pathogens |
spellingShingle | Sylwia Zielińska Adam Matkowski Karolina Dydak Monika Ewa Czerwińska Magdalena Dziągwa-Becker Mariusz Kucharski Magdalena Wójciak Ireneusz Sowa Stanisława Plińska Karol Fijałkowski Daria Ciecholewska-Juśko Michał Broda Damian Gorczyca Adam Junka Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures Materials bacterial nanocellulose <i>Chelidonium majus</i> cell culture carrier phenolic compounds isoquinoline alkaloids microbial pathogens |
title | Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures |
title_full | Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures |
title_fullStr | Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures |
title_full_unstemmed | Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures |
title_short | Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from <i>Chelidonium majus</i> Plant Cell Cultures |
title_sort | bacterial nanocellulose fortified with antimicrobial and anti inflammatory natural products from i chelidonium majus i plant cell cultures |
topic | bacterial nanocellulose <i>Chelidonium majus</i> cell culture carrier phenolic compounds isoquinoline alkaloids microbial pathogens |
url | https://www.mdpi.com/1996-1944/15/1/16 |
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