Transcriptome profiling of avian pathogenic Escherichia coli and the mouse microvascular endothelial cell line bEnd.3 during interaction
Background Avian pathogenic Escherichia coli (APEC), an important extraintestinal pathogenic E. coli, causes colibacillosis, an acute and mostly systemic disease involving multiple organ lesions such as meningitis. Meningitis-causing APEC can invade the host central nervous system by crossing the bl...
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PeerJ Inc.
2020-05-01
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| Online Access: | https://peerj.com/articles/9172.pdf |
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| author | Peili Wang Xia Meng Jianji Li Yanfei Chen Dong Zhang Haoran Zhong Pengpeng Xia Luying Cui Guoqiang Zhu Heng Wang |
| author_facet | Peili Wang Xia Meng Jianji Li Yanfei Chen Dong Zhang Haoran Zhong Pengpeng Xia Luying Cui Guoqiang Zhu Heng Wang |
| author_sort | Peili Wang |
| collection | DOAJ |
| description | Background Avian pathogenic Escherichia coli (APEC), an important extraintestinal pathogenic E. coli, causes colibacillosis, an acute and mostly systemic disease involving multiple organ lesions such as meningitis. Meningitis-causing APEC can invade the host central nervous system by crossing the blood–brain barrier (BBB), which is a critical step in the development of meningitis. However, the bacteria-host interaction mechanism in this process remains unclear. Methods In this study, we examined E. coli and bEnd.3 cells transcriptomes during infection and mock infection to investigate the global transcriptional changes in both organisms using RNA sequencing approach. Results When APEC infected the bEnd.3 cells, several significant changes in the expression of genes related to cell junctional complexes, extracellular matrix degradation, actin cytoskeleton rearrangement, immune activation and the inflammatory response in bEnd.3 cells were observed as compared to the mock infection group. Thus, the immune activation of bEnd.3 cells indicated that APEC infection activated host defenses. Furthermore, APEC may exploit cell junction degradation to invade the BBB. In addition, amino acid metabolism and energy metabolism related genes were downregulated and the protein export pathway related genes were upregulated in APEC cultured with bEnd.3 cells, compared to that in control. Thus, APEC may encounter starvation and express virulence factors during incubation with bEnd.3 cells. Conclusion This study provides a comprehensive overview of transcriptomic changes that occur during APEC infection of bEnd.3 cells, and offers insights into the bacterial invasion strategies and the subsequent host defense mechanism. |
| first_indexed | 2024-03-09T06:47:22Z |
| format | Article |
| id | doaj.art-86e32304d5b94488a2bd3500f2902633 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:47:22Z |
| publishDate | 2020-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-86e32304d5b94488a2bd3500f29026332023-12-03T10:34:17ZengPeerJ Inc.PeerJ2167-83592020-05-018e917210.7717/peerj.9172Transcriptome profiling of avian pathogenic Escherichia coli and the mouse microvascular endothelial cell line bEnd.3 during interactionPeili Wang0Xia Meng1Jianji Li2Yanfei Chen3Dong Zhang4Haoran Zhong5Pengpeng Xia6Luying Cui7Guoqiang Zhu8Heng Wang9College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, ChinaBackground Avian pathogenic Escherichia coli (APEC), an important extraintestinal pathogenic E. coli, causes colibacillosis, an acute and mostly systemic disease involving multiple organ lesions such as meningitis. Meningitis-causing APEC can invade the host central nervous system by crossing the blood–brain barrier (BBB), which is a critical step in the development of meningitis. However, the bacteria-host interaction mechanism in this process remains unclear. Methods In this study, we examined E. coli and bEnd.3 cells transcriptomes during infection and mock infection to investigate the global transcriptional changes in both organisms using RNA sequencing approach. Results When APEC infected the bEnd.3 cells, several significant changes in the expression of genes related to cell junctional complexes, extracellular matrix degradation, actin cytoskeleton rearrangement, immune activation and the inflammatory response in bEnd.3 cells were observed as compared to the mock infection group. Thus, the immune activation of bEnd.3 cells indicated that APEC infection activated host defenses. Furthermore, APEC may exploit cell junction degradation to invade the BBB. In addition, amino acid metabolism and energy metabolism related genes were downregulated and the protein export pathway related genes were upregulated in APEC cultured with bEnd.3 cells, compared to that in control. Thus, APEC may encounter starvation and express virulence factors during incubation with bEnd.3 cells. Conclusion This study provides a comprehensive overview of transcriptomic changes that occur during APEC infection of bEnd.3 cells, and offers insights into the bacterial invasion strategies and the subsequent host defense mechanism.https://peerj.com/articles/9172.pdfDual RNA-seqHost–pathogen interactionsMeningitisAPECbEnd.3 cells |
| spellingShingle | Peili Wang Xia Meng Jianji Li Yanfei Chen Dong Zhang Haoran Zhong Pengpeng Xia Luying Cui Guoqiang Zhu Heng Wang Transcriptome profiling of avian pathogenic Escherichia coli and the mouse microvascular endothelial cell line bEnd.3 during interaction PeerJ Dual RNA-seq Host–pathogen interactions Meningitis APEC bEnd.3 cells |
| title | Transcriptome profiling of avian pathogenic Escherichia coli and the mouse microvascular endothelial cell line bEnd.3 during interaction |
| title_full | Transcriptome profiling of avian pathogenic Escherichia coli and the mouse microvascular endothelial cell line bEnd.3 during interaction |
| title_fullStr | Transcriptome profiling of avian pathogenic Escherichia coli and the mouse microvascular endothelial cell line bEnd.3 during interaction |
| title_full_unstemmed | Transcriptome profiling of avian pathogenic Escherichia coli and the mouse microvascular endothelial cell line bEnd.3 during interaction |
| title_short | Transcriptome profiling of avian pathogenic Escherichia coli and the mouse microvascular endothelial cell line bEnd.3 during interaction |
| title_sort | transcriptome profiling of avian pathogenic escherichia coli and the mouse microvascular endothelial cell line bend 3 during interaction |
| topic | Dual RNA-seq Host–pathogen interactions Meningitis APEC bEnd.3 cells |
| url | https://peerj.com/articles/9172.pdf |
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