Highly Sensitive Fluorescent Biosensor Based on Acetylcholinesterase and Carbon Dots–Graphene Oxide Quenching Test for Analytical and Commercial Organophosphate Pesticide Detection
Chlorpyrifos formulations are the most used in Colombia and other countries for crop protection, and their presence has been proven in many water sources. The application of nanomaterials, including carbon quantum dots (CD), can significantly improve the performance of optical biosensors for quick a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Environmental Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenvs.2022.825112/full |
| _version_ | 1819120673092534272 |
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| author | Maria I. Gaviria Kaory Barrientos Juan Pablo Arango Juan B. Cano Gustavo A. Peñuela |
| author_facet | Maria I. Gaviria Kaory Barrientos Juan Pablo Arango Juan B. Cano Gustavo A. Peñuela |
| author_sort | Maria I. Gaviria |
| collection | DOAJ |
| description | Chlorpyrifos formulations are the most used in Colombia and other countries for crop protection, and their presence has been proven in many water sources. The application of nanomaterials, including carbon quantum dots (CD), can significantly improve the performance of optical biosensors for quick and accurate detection of these pesticides. In this work, naturally fluorescent and nontoxic CD were conjugated with acetylcholinesterase (AChE) as a bioreceptor, to produce a fluorescent biosensor. This system was modulated with graphene oxide (GO), showing a fluorescence recovery in the presence of the pesticide. The biosensor was evaluated for the detection of pure chlorpyrifos (CPF), profenofos (PF), and a commercial formulation called Lorsban®. A limit of detection (LOD) as low as 0.14 and 2.05 ppb for chlorpyrifos and Lorsban®, respectively, was obtained. Profenofos did not show any recovery with this system at the evaluated concentrations. The system also showed a good selectivity in the presence of proteins (BSA) and other organic substances (Glucose) usually present in drinking water. The specificity was also evaluated against cypermethrin and methomyl commercial formulations showing minimal interference. To the best of our knowledge, this is one of the few works reporting the detection of commercial pesticide formulations with promising results. |
| first_indexed | 2024-12-22T06:24:24Z |
| format | Article |
| id | doaj.art-86e580e9f1f74a2e849e71050b1f76e4 |
| institution | Directory Open Access Journal |
| issn | 2296-665X |
| language | English |
| last_indexed | 2024-12-22T06:24:24Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Environmental Science |
| spelling | doaj.art-86e580e9f1f74a2e849e71050b1f76e42022-12-21T18:35:53ZengFrontiers Media S.A.Frontiers in Environmental Science2296-665X2022-03-011010.3389/fenvs.2022.825112825112Highly Sensitive Fluorescent Biosensor Based on Acetylcholinesterase and Carbon Dots–Graphene Oxide Quenching Test for Analytical and Commercial Organophosphate Pesticide DetectionMaria I. Gaviria0Kaory Barrientos1Juan Pablo Arango2Juan B. Cano3Gustavo A. Peñuela4GDCON Research Group, Engineering Faculty, Universidad de Antioquia, Medellín, ColombiaGIBEC Research Group, Life Sciences Faculty, Universidad EIA, Medellín, ColombiaGIBEC Research Group, Life Sciences Faculty, Universidad EIA, Medellín, ColombiaGIMEL Research Group, Engineering Faculty, Universidad de Antioquia, Medellín, ColombiaGDCON Research Group, Engineering Faculty, Universidad de Antioquia, Medellín, ColombiaChlorpyrifos formulations are the most used in Colombia and other countries for crop protection, and their presence has been proven in many water sources. The application of nanomaterials, including carbon quantum dots (CD), can significantly improve the performance of optical biosensors for quick and accurate detection of these pesticides. In this work, naturally fluorescent and nontoxic CD were conjugated with acetylcholinesterase (AChE) as a bioreceptor, to produce a fluorescent biosensor. This system was modulated with graphene oxide (GO), showing a fluorescence recovery in the presence of the pesticide. The biosensor was evaluated for the detection of pure chlorpyrifos (CPF), profenofos (PF), and a commercial formulation called Lorsban®. A limit of detection (LOD) as low as 0.14 and 2.05 ppb for chlorpyrifos and Lorsban®, respectively, was obtained. Profenofos did not show any recovery with this system at the evaluated concentrations. The system also showed a good selectivity in the presence of proteins (BSA) and other organic substances (Glucose) usually present in drinking water. The specificity was also evaluated against cypermethrin and methomyl commercial formulations showing minimal interference. To the best of our knowledge, this is one of the few works reporting the detection of commercial pesticide formulations with promising results.https://www.frontiersin.org/articles/10.3389/fenvs.2022.825112/fullcarbon dotsnanomaterialfluorescent biosensorpesticidechlorpyrifosenzyme |
| spellingShingle | Maria I. Gaviria Kaory Barrientos Juan Pablo Arango Juan B. Cano Gustavo A. Peñuela Highly Sensitive Fluorescent Biosensor Based on Acetylcholinesterase and Carbon Dots–Graphene Oxide Quenching Test for Analytical and Commercial Organophosphate Pesticide Detection Frontiers in Environmental Science carbon dots nanomaterial fluorescent biosensor pesticide chlorpyrifos enzyme |
| title | Highly Sensitive Fluorescent Biosensor Based on Acetylcholinesterase and Carbon Dots–Graphene Oxide Quenching Test for Analytical and Commercial Organophosphate Pesticide Detection |
| title_full | Highly Sensitive Fluorescent Biosensor Based on Acetylcholinesterase and Carbon Dots–Graphene Oxide Quenching Test for Analytical and Commercial Organophosphate Pesticide Detection |
| title_fullStr | Highly Sensitive Fluorescent Biosensor Based on Acetylcholinesterase and Carbon Dots–Graphene Oxide Quenching Test for Analytical and Commercial Organophosphate Pesticide Detection |
| title_full_unstemmed | Highly Sensitive Fluorescent Biosensor Based on Acetylcholinesterase and Carbon Dots–Graphene Oxide Quenching Test for Analytical and Commercial Organophosphate Pesticide Detection |
| title_short | Highly Sensitive Fluorescent Biosensor Based on Acetylcholinesterase and Carbon Dots–Graphene Oxide Quenching Test for Analytical and Commercial Organophosphate Pesticide Detection |
| title_sort | highly sensitive fluorescent biosensor based on acetylcholinesterase and carbon dots graphene oxide quenching test for analytical and commercial organophosphate pesticide detection |
| topic | carbon dots nanomaterial fluorescent biosensor pesticide chlorpyrifos enzyme |
| url | https://www.frontiersin.org/articles/10.3389/fenvs.2022.825112/full |
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