Isolation, Purification and Structural Characterization of Polysaccharides from Chickpea
In this research, the distribution and physicochemical properties of polysaccharides from the whole seed, cotyledon and hull of Cicer arietinum were analyzed and compared, and the structure characteristics of Cicer arietinum hull polysaccharide (CAHP) were characterized. It was found that polysaccha...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-04-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-8-006.pdf |
| _version_ | 1797805564567224320 |
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| author | ZHAO Jiaying, XIN Yue, SONG Xiaoxiao, NIE Shaoping, YIN Junyi |
| author_facet | ZHAO Jiaying, XIN Yue, SONG Xiaoxiao, NIE Shaoping, YIN Junyi |
| author_sort | ZHAO Jiaying, XIN Yue, SONG Xiaoxiao, NIE Shaoping, YIN Junyi |
| collection | DOAJ |
| description | In this research, the distribution and physicochemical properties of polysaccharides from the whole seed, cotyledon and hull of Cicer arietinum were analyzed and compared, and the structure characteristics of Cicer arietinum hull polysaccharide (CAHP) were characterized. It was found that polysaccharide was concentrated in the hull of chickpea according to the yield and composition of polysaccharide. CAHP was further purified by column chromatography into six fractions (Fw, F0.1, F0.2, F0.3, F0.5 and F2), and the structure of CAHP-F0.2, which showed relatively high yield and good homogeneity, was characterized. The results showed that CAHP-F0.2 was consisted of mostly homogalacturonan (HG)-type pectic polysaccharide whose main chain was linked by 1,4-GalpA, and contained a small amount of RG-I domain, with arabincan and arabinogalactan-II side chain. This study will provide a theoretical basis for future research on the structure, biological activity and resource development of chickpea polysaccharides. |
| first_indexed | 2024-03-13T05:53:57Z |
| format | Article |
| id | doaj.art-86ea3a6fafae4239ab445ce6b3f8199c |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-13T05:53:57Z |
| publishDate | 2023-04-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-86ea3a6fafae4239ab445ce6b3f8199c2023-06-13T07:42:04ZengChina Food Publishing CompanyShipin Kexue1002-66302023-04-01448404510.7506/spkx1002-6630-20220816-190Isolation, Purification and Structural Characterization of Polysaccharides from ChickpeaZHAO Jiaying, XIN Yue, SONG Xiaoxiao, NIE Shaoping, YIN Junyi0(State Key Laboratory of Food Science and Technology, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Key Laboratory of Bioactive Polysaccharides of Jiangxi Province, Nanchang University, Nanchang 330047, China)In this research, the distribution and physicochemical properties of polysaccharides from the whole seed, cotyledon and hull of Cicer arietinum were analyzed and compared, and the structure characteristics of Cicer arietinum hull polysaccharide (CAHP) were characterized. It was found that polysaccharide was concentrated in the hull of chickpea according to the yield and composition of polysaccharide. CAHP was further purified by column chromatography into six fractions (Fw, F0.1, F0.2, F0.3, F0.5 and F2), and the structure of CAHP-F0.2, which showed relatively high yield and good homogeneity, was characterized. The results showed that CAHP-F0.2 was consisted of mostly homogalacturonan (HG)-type pectic polysaccharide whose main chain was linked by 1,4-GalpA, and contained a small amount of RG-I domain, with arabincan and arabinogalactan-II side chain. This study will provide a theoretical basis for future research on the structure, biological activity and resource development of chickpea polysaccharides.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-8-006.pdfchickpea; polysaccharide; separation and purification; structural characteristics |
| spellingShingle | ZHAO Jiaying, XIN Yue, SONG Xiaoxiao, NIE Shaoping, YIN Junyi Isolation, Purification and Structural Characterization of Polysaccharides from Chickpea Shipin Kexue chickpea; polysaccharide; separation and purification; structural characteristics |
| title | Isolation, Purification and Structural Characterization of Polysaccharides from Chickpea |
| title_full | Isolation, Purification and Structural Characterization of Polysaccharides from Chickpea |
| title_fullStr | Isolation, Purification and Structural Characterization of Polysaccharides from Chickpea |
| title_full_unstemmed | Isolation, Purification and Structural Characterization of Polysaccharides from Chickpea |
| title_short | Isolation, Purification and Structural Characterization of Polysaccharides from Chickpea |
| title_sort | isolation purification and structural characterization of polysaccharides from chickpea |
| topic | chickpea; polysaccharide; separation and purification; structural characteristics |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-8-006.pdf |
| work_keys_str_mv | AT zhaojiayingxinyuesongxiaoxiaonieshaopingyinjunyi isolationpurificationandstructuralcharacterizationofpolysaccharidesfromchickpea |