Raw data for the identification of SUMOylated proteins in S. cerevisiae subjected to two types of osmotic shock, using affinity purification coupled with mass spectrometry
The small ubiquitin-related modifier (SUMO) “stress response” (SSR) is a poorly understood evolutionarily conserved phenomenon in which steady-state SUMO conjugate levels are dramatically increased in response to environmental stresses. Here we describe the data acquired using affinity-purification...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-03-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340914000274 |
| _version_ | 1818445015811096576 |
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| author | Tharan Srikumar Megan C. Lewicki Brian Raught |
| author_facet | Tharan Srikumar Megan C. Lewicki Brian Raught |
| author_sort | Tharan Srikumar |
| collection | DOAJ |
| description | The small ubiquitin-related modifier (SUMO) “stress response” (SSR) is a poorly understood evolutionarily conserved phenomenon in which steady-state SUMO conjugate levels are dramatically increased in response to environmental stresses. Here we describe the data acquired using affinity-purification coupled with mass spectrometry to identify proteins that are SUMOylated in response to two different types of osmotic stress, 1 M sorbitol and 1 M KCl. The mass spectrometry dataset described here has been uploaded to the MassIVE repository with ID: MSV000078739, and consists of 32 raw MS files acquired in data-dependent mode on a Thermo Q-Exactive instrument. iProphet-processed MS/MS search results and associated SAINT scores are also included as a reference. These data are discussed and interpreted in “The S. cerevisiae SUMO stress response is a conjugation–deconjugation cycle that targets the transcription machinery”, by Lewicki et al. in the Journal of Proteomics, 2014 [1]. |
| first_indexed | 2024-12-14T19:25:07Z |
| format | Article |
| id | doaj.art-86ebf564e6ce4bfda224bb231ff84706 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-14T19:25:07Z |
| publishDate | 2015-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-86ebf564e6ce4bfda224bb231ff847062022-12-21T22:50:13ZengElsevierData in Brief2352-34092015-03-012C293110.1016/j.dib.2014.11.003Raw data for the identification of SUMOylated proteins in S. cerevisiae subjected to two types of osmotic shock, using affinity purification coupled with mass spectrometryTharan Srikumar0Megan C. Lewicki1Brian Raught2Princess Margaret Cancer Centre, University Health Network, CanadaPrincess Margaret Cancer Centre, University Health Network, CanadaPrincess Margaret Cancer Centre, University Health Network, CanadaThe small ubiquitin-related modifier (SUMO) “stress response” (SSR) is a poorly understood evolutionarily conserved phenomenon in which steady-state SUMO conjugate levels are dramatically increased in response to environmental stresses. Here we describe the data acquired using affinity-purification coupled with mass spectrometry to identify proteins that are SUMOylated in response to two different types of osmotic stress, 1 M sorbitol and 1 M KCl. The mass spectrometry dataset described here has been uploaded to the MassIVE repository with ID: MSV000078739, and consists of 32 raw MS files acquired in data-dependent mode on a Thermo Q-Exactive instrument. iProphet-processed MS/MS search results and associated SAINT scores are also included as a reference. These data are discussed and interpreted in “The S. cerevisiae SUMO stress response is a conjugation–deconjugation cycle that targets the transcription machinery”, by Lewicki et al. in the Journal of Proteomics, 2014 [1].http://www.sciencedirect.com/science/article/pii/S2352340914000274 |
| spellingShingle | Tharan Srikumar Megan C. Lewicki Brian Raught Raw data for the identification of SUMOylated proteins in S. cerevisiae subjected to two types of osmotic shock, using affinity purification coupled with mass spectrometry Data in Brief |
| title | Raw data for the identification of SUMOylated proteins in S. cerevisiae subjected to two types of osmotic shock, using affinity purification coupled with mass spectrometry |
| title_full | Raw data for the identification of SUMOylated proteins in S. cerevisiae subjected to two types of osmotic shock, using affinity purification coupled with mass spectrometry |
| title_fullStr | Raw data for the identification of SUMOylated proteins in S. cerevisiae subjected to two types of osmotic shock, using affinity purification coupled with mass spectrometry |
| title_full_unstemmed | Raw data for the identification of SUMOylated proteins in S. cerevisiae subjected to two types of osmotic shock, using affinity purification coupled with mass spectrometry |
| title_short | Raw data for the identification of SUMOylated proteins in S. cerevisiae subjected to two types of osmotic shock, using affinity purification coupled with mass spectrometry |
| title_sort | raw data for the identification of sumoylated proteins in s cerevisiae subjected to two types of osmotic shock using affinity purification coupled with mass spectrometry |
| url | http://www.sciencedirect.com/science/article/pii/S2352340914000274 |
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