PCR Primer Specific CaMV 35S Promoter to Detect Transgenic Soybean in Indonesia Commercial Soy Bean and Tempeh
In the framework of the supervision and enforcement of the regulation regarding the content of soybean transgenic in food and processed foods such as tempeh, a reliable testing method is indispensable. Performance specific primer PCR amplification with promoter of CaMV 35S tested to detect the prese...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Department of Chemistry, Universitas Gadjah Mada
2017-11-01
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| Series: | Indonesian Journal of Chemistry |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/24163 |
| _version_ | 1819117535703859200 |
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| author | Tri Joko Raharjo Surajiman Surajiman |
| author_facet | Tri Joko Raharjo Surajiman Surajiman |
| author_sort | Tri Joko Raharjo |
| collection | DOAJ |
| description | In the framework of the supervision and enforcement of the regulation regarding the content of soybean transgenic in food and processed foods such as tempeh, a reliable testing method is indispensable. Performance specific primer PCR amplification with promoter of CaMV 35S tested to detect the presence of GMOs. The parameters tested were specificity, precision and cut off detection using CRM transgenic soybean. The method is reliable to detect transgenic soybean specifically and has the annealing temperature at 59 °C during the 30 cycle standard PCR condition. The method did not show any false positive and false negative results meaning good precision. The cut off the methods is up to 2 copies total DNA of soybean or less than 104 copies of the CaMV 35S promoter. Observation to the commercial soybeans and tempeh found that most of the commercially available soybean in Indonesia are transgenic (8 of 10 sample) while all tested tempeh sample were detected have been fermented from transgenic soybeans. |
| first_indexed | 2024-12-22T05:34:32Z |
| format | Article |
| id | doaj.art-86ec31a71f254277802e3d0a8121a4cb |
| institution | Directory Open Access Journal |
| issn | 1411-9420 2460-1578 |
| language | English |
| last_indexed | 2024-12-22T05:34:32Z |
| publishDate | 2017-11-01 |
| publisher | Department of Chemistry, Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Chemistry |
| spelling | doaj.art-86ec31a71f254277802e3d0a8121a4cb2022-12-21T18:37:21ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782017-11-0117341542110.22146/ijc.2416319131PCR Primer Specific CaMV 35S Promoter to Detect Transgenic Soybean in Indonesia Commercial Soy Bean and TempehTri Joko Raharjo0Surajiman Surajiman1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, IndonesiaIntegrated Research and Testing Laboratory, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, IndonesiaIn the framework of the supervision and enforcement of the regulation regarding the content of soybean transgenic in food and processed foods such as tempeh, a reliable testing method is indispensable. Performance specific primer PCR amplification with promoter of CaMV 35S tested to detect the presence of GMOs. The parameters tested were specificity, precision and cut off detection using CRM transgenic soybean. The method is reliable to detect transgenic soybean specifically and has the annealing temperature at 59 °C during the 30 cycle standard PCR condition. The method did not show any false positive and false negative results meaning good precision. The cut off the methods is up to 2 copies total DNA of soybean or less than 104 copies of the CaMV 35S promoter. Observation to the commercial soybeans and tempeh found that most of the commercially available soybean in Indonesia are transgenic (8 of 10 sample) while all tested tempeh sample were detected have been fermented from transgenic soybeans.https://jurnal.ugm.ac.id/ijc/article/view/24163transgenictempehsoybeansPCRCaMV 35S promoter |
| spellingShingle | Tri Joko Raharjo Surajiman Surajiman PCR Primer Specific CaMV 35S Promoter to Detect Transgenic Soybean in Indonesia Commercial Soy Bean and Tempeh Indonesian Journal of Chemistry transgenic tempeh soybeans PCR CaMV 35S promoter |
| title | PCR Primer Specific CaMV 35S Promoter to Detect Transgenic Soybean in Indonesia Commercial Soy Bean and Tempeh |
| title_full | PCR Primer Specific CaMV 35S Promoter to Detect Transgenic Soybean in Indonesia Commercial Soy Bean and Tempeh |
| title_fullStr | PCR Primer Specific CaMV 35S Promoter to Detect Transgenic Soybean in Indonesia Commercial Soy Bean and Tempeh |
| title_full_unstemmed | PCR Primer Specific CaMV 35S Promoter to Detect Transgenic Soybean in Indonesia Commercial Soy Bean and Tempeh |
| title_short | PCR Primer Specific CaMV 35S Promoter to Detect Transgenic Soybean in Indonesia Commercial Soy Bean and Tempeh |
| title_sort | pcr primer specific camv 35s promoter to detect transgenic soybean in indonesia commercial soy bean and tempeh |
| topic | transgenic tempeh soybeans PCR CaMV 35S promoter |
| url | https://jurnal.ugm.ac.id/ijc/article/view/24163 |
| work_keys_str_mv | AT trijokoraharjo pcrprimerspecificcamv35spromotertodetecttransgenicsoybeaninindonesiacommercialsoybeanandtempeh AT surajimansurajiman pcrprimerspecificcamv35spromotertodetecttransgenicsoybeaninindonesiacommercialsoybeanandtempeh |