Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips.
The coronary artery ligation model in rodents mimics human myocardial infarction (MI). Normally mechanical ventilation and prolonged anesthesia period are needed. Recently, a method has been developed to create MI by popping-out the heart (without ventilation) followed by immediate suture ligation....
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4658059?pdf=render |
| _version_ | 1819078535074021376 |
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| author | James N B M de Andrade Junnan Tang Michael Taylor Hensley Adam Vandergriff Jhon Cores Eric Henry Tyler A Allen Thomas George Caranasos Zegen Wang Tianxia Zhang Jinying Zhang Ke Cheng |
| author_facet | James N B M de Andrade Junnan Tang Michael Taylor Hensley Adam Vandergriff Jhon Cores Eric Henry Tyler A Allen Thomas George Caranasos Zegen Wang Tianxia Zhang Jinying Zhang Ke Cheng |
| author_sort | James N B M de Andrade |
| collection | DOAJ |
| description | The coronary artery ligation model in rodents mimics human myocardial infarction (MI). Normally mechanical ventilation and prolonged anesthesia period are needed. Recently, a method has been developed to create MI by popping-out the heart (without ventilation) followed by immediate suture ligation. Mortality is high due to the time-consuming suture ligation process while the heart is exposed. We sought to improve this method and reduce mortality by rapid coronary ligation using a surgical clip instead of a suture.Mice were randomized into 3 groups: clip MI (CMI), suture MI (SMI), or sham (SHAM). In all groups, heart was manually exposed without intubation through a small incision on the chest wall. Unlike the conventional SMI method, mice in the CMI group received a metal clip on left anterior descending artery (LAD), quickly dispensed by an AutoSuture Surgiclip™. The CMI method took only 1/3 of ligation time of the standard SMI method and improved post-MI survival rate. TTC staining and Masson's trichrome staining revealed a similar degree of infarct size in the SMI and CMI groups. Echocardiograph confirmed that both SMI and CMI groups had a similar reduction of ejection fraction and fraction shortening over the time. Histological analysis showed that the numbers of CD68+ macrophages and apoptotic cells (TUNEL-positive) are indistinguishable between the two groups.This new method, taking only less than 3 minutes to complete, represents an efficient myocardial infarction model in rodents. |
| first_indexed | 2024-12-21T19:14:38Z |
| format | Article |
| id | doaj.art-86eed75bc0294cfe98ed195c820a24c1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T19:14:38Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-86eed75bc0294cfe98ed195c820a24c12022-12-21T18:53:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014322110.1371/journal.pone.0143221Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips.James N B M de AndradeJunnan TangMichael Taylor HensleyAdam VandergriffJhon CoresEric HenryTyler A AllenThomas George CaranasosZegen WangTianxia ZhangJinying ZhangKe ChengThe coronary artery ligation model in rodents mimics human myocardial infarction (MI). Normally mechanical ventilation and prolonged anesthesia period are needed. Recently, a method has been developed to create MI by popping-out the heart (without ventilation) followed by immediate suture ligation. Mortality is high due to the time-consuming suture ligation process while the heart is exposed. We sought to improve this method and reduce mortality by rapid coronary ligation using a surgical clip instead of a suture.Mice were randomized into 3 groups: clip MI (CMI), suture MI (SMI), or sham (SHAM). In all groups, heart was manually exposed without intubation through a small incision on the chest wall. Unlike the conventional SMI method, mice in the CMI group received a metal clip on left anterior descending artery (LAD), quickly dispensed by an AutoSuture Surgiclip™. The CMI method took only 1/3 of ligation time of the standard SMI method and improved post-MI survival rate. TTC staining and Masson's trichrome staining revealed a similar degree of infarct size in the SMI and CMI groups. Echocardiograph confirmed that both SMI and CMI groups had a similar reduction of ejection fraction and fraction shortening over the time. Histological analysis showed that the numbers of CD68+ macrophages and apoptotic cells (TUNEL-positive) are indistinguishable between the two groups.This new method, taking only less than 3 minutes to complete, represents an efficient myocardial infarction model in rodents.http://europepmc.org/articles/PMC4658059?pdf=render |
| spellingShingle | James N B M de Andrade Junnan Tang Michael Taylor Hensley Adam Vandergriff Jhon Cores Eric Henry Tyler A Allen Thomas George Caranasos Zegen Wang Tianxia Zhang Jinying Zhang Ke Cheng Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips. PLoS ONE |
| title | Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips. |
| title_full | Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips. |
| title_fullStr | Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips. |
| title_full_unstemmed | Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips. |
| title_short | Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips. |
| title_sort | rapid and efficient production of coronary artery ligation and myocardial infarction in mice using surgical clips |
| url | http://europepmc.org/articles/PMC4658059?pdf=render |
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