The Experimental Infections of the Human Isolate of <i>Strongyloides Stercoralis</i> in a Rodent Model (The Mongolian Gerbil, <i>Meriones Unguiculatus</i>)
Strongyloidiasis is life-threatening disease which is mainly caused by <i>Strongyloides stercoralis</i> infection. Autoinfection of the parasite results in long-lasting infection and fatal conditions, hyperinfection and dissemination (primarily in immunosuppressed hosts). However, mechan...
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MDPI AG
2019-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/8/1/21 |
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| author | Sarit Charuchaibovorn Vivornpun Sanprasert Surang Nuchprayoon |
| author_facet | Sarit Charuchaibovorn Vivornpun Sanprasert Surang Nuchprayoon |
| author_sort | Sarit Charuchaibovorn |
| collection | DOAJ |
| description | Strongyloidiasis is life-threatening disease which is mainly caused by <i>Strongyloides stercoralis</i> infection. Autoinfection of the parasite results in long-lasting infection and fatal conditions, hyperinfection and dissemination (primarily in immunosuppressed hosts). However, mechanisms of autoinfection and biology remain largely unknown. Rodent models including mice and rats are not susceptible to the human isolate of <i>S. stercoralis</i>. Variations in susceptibility of the human isolate of <i>S. stercoralis</i> are found in dogs. <i>S. ratti</i> and <i>S. venezuelensis</i> infections in rats are an alternative model without the ability to cause autoinfection. The absence of appropriate model for the human isolate of strongyloidiasis hampers a better understanding of human strongyloidiasis. We demonstrated the maintenance of the human isolate of the <i>S. stercoralis</i> life cycle in the Mongolian gerbil (<i>Meriones unguiculatus</i>). The human isolate of <i>S. stercoralis</i> caused a patent infection in immunosuppressed gerbils, more than 18 months. The mean number of recovery adult parasitic worms were 120 ± 23 (1.2% of the initial dose) and L1s were 12,500 ± 7500 after day 28 post-inoculation (p.i.). The prepatent period was 9⁻14 days. Mild diarrhoea was found in gerbils carrying a high number of adult parasitic worms. Our findings provided a promising model for studying biology and searching new alternative drugs against the parasites. Further studies about the hyperinfection and dissemination would be performed. |
| first_indexed | 2024-04-14T03:28:14Z |
| format | Article |
| id | doaj.art-86f172976bda4225ad983031340c993f |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-14T03:28:14Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-86f172976bda4225ad983031340c993f2022-12-22T02:15:04ZengMDPI AGPathogens2076-08172019-02-01812110.3390/pathogens8010021pathogens8010021The Experimental Infections of the Human Isolate of <i>Strongyloides Stercoralis</i> in a Rodent Model (The Mongolian Gerbil, <i>Meriones Unguiculatus</i>)Sarit Charuchaibovorn0Vivornpun Sanprasert1Surang Nuchprayoon2Medical Sciences Program, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandLymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandLymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandStrongyloidiasis is life-threatening disease which is mainly caused by <i>Strongyloides stercoralis</i> infection. Autoinfection of the parasite results in long-lasting infection and fatal conditions, hyperinfection and dissemination (primarily in immunosuppressed hosts). However, mechanisms of autoinfection and biology remain largely unknown. Rodent models including mice and rats are not susceptible to the human isolate of <i>S. stercoralis</i>. Variations in susceptibility of the human isolate of <i>S. stercoralis</i> are found in dogs. <i>S. ratti</i> and <i>S. venezuelensis</i> infections in rats are an alternative model without the ability to cause autoinfection. The absence of appropriate model for the human isolate of strongyloidiasis hampers a better understanding of human strongyloidiasis. We demonstrated the maintenance of the human isolate of the <i>S. stercoralis</i> life cycle in the Mongolian gerbil (<i>Meriones unguiculatus</i>). The human isolate of <i>S. stercoralis</i> caused a patent infection in immunosuppressed gerbils, more than 18 months. The mean number of recovery adult parasitic worms were 120 ± 23 (1.2% of the initial dose) and L1s were 12,500 ± 7500 after day 28 post-inoculation (p.i.). The prepatent period was 9⁻14 days. Mild diarrhoea was found in gerbils carrying a high number of adult parasitic worms. Our findings provided a promising model for studying biology and searching new alternative drugs against the parasites. Further studies about the hyperinfection and dissemination would be performed.https://www.mdpi.com/2076-0817/8/1/21strongyloidiasis<i>Strongyloides stercoralis</i>Mongolian gerbil (<i>Meriones unguiculatus</i>)hyperinfectiondissemination |
| spellingShingle | Sarit Charuchaibovorn Vivornpun Sanprasert Surang Nuchprayoon The Experimental Infections of the Human Isolate of <i>Strongyloides Stercoralis</i> in a Rodent Model (The Mongolian Gerbil, <i>Meriones Unguiculatus</i>) Pathogens strongyloidiasis <i>Strongyloides stercoralis</i> Mongolian gerbil (<i>Meriones unguiculatus</i>) hyperinfection dissemination |
| title | The Experimental Infections of the Human Isolate of <i>Strongyloides Stercoralis</i> in a Rodent Model (The Mongolian Gerbil, <i>Meriones Unguiculatus</i>) |
| title_full | The Experimental Infections of the Human Isolate of <i>Strongyloides Stercoralis</i> in a Rodent Model (The Mongolian Gerbil, <i>Meriones Unguiculatus</i>) |
| title_fullStr | The Experimental Infections of the Human Isolate of <i>Strongyloides Stercoralis</i> in a Rodent Model (The Mongolian Gerbil, <i>Meriones Unguiculatus</i>) |
| title_full_unstemmed | The Experimental Infections of the Human Isolate of <i>Strongyloides Stercoralis</i> in a Rodent Model (The Mongolian Gerbil, <i>Meriones Unguiculatus</i>) |
| title_short | The Experimental Infections of the Human Isolate of <i>Strongyloides Stercoralis</i> in a Rodent Model (The Mongolian Gerbil, <i>Meriones Unguiculatus</i>) |
| title_sort | experimental infections of the human isolate of i strongyloides stercoralis i in a rodent model the mongolian gerbil i meriones unguiculatus i |
| topic | strongyloidiasis <i>Strongyloides stercoralis</i> Mongolian gerbil (<i>Meriones unguiculatus</i>) hyperinfection dissemination |
| url | https://www.mdpi.com/2076-0817/8/1/21 |
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