POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS
Diabetes mellitus is a multifactorial disease. It has recently been shown that an insertion (I)/deletion (D) polymorphism exists in the angiotensin-converting enzyme (ACE) gene that can affect the serum ACE level. There are three genotypes: DD, DI, and II, with the ACE level being highest in DD, int...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2008-08-01
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| Series: | Acta Medica Iranica |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/3482 |
| _version_ | 1811264961766227968 |
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| author | A Nikzamir M Nakhjavani T Golmohammadi L Dibai R Saffary |
| author_facet | A Nikzamir M Nakhjavani T Golmohammadi L Dibai R Saffary |
| author_sort | A Nikzamir |
| collection | DOAJ |
| description | Diabetes mellitus is a multifactorial disease. It has recently been shown that an insertion (I)/deletion (D) polymorphism exists in the angiotensin-converting enzyme (ACE) gene that can affect the serum ACE level. There are three genotypes: DD, DI, and II, with the ACE level being highest in DD, intermediate in DI, and lowest in II. In the present investigation, 170 patients with type 2 diabetes mellitus (T2DM) and 144 control subjects were studied. The ACE I/D polymorphism was determined by polymerase chain reaction (PCR) utilizing specific primers. ACE activity was determined spectrophotometrically. Distribution of ACE gene (I/D) polymorphism and allele frequencies in patients with T2DM were significantly different from those in control (P < 0.001); D allele frequency was 51% in T2DM vs. 48% in controls. The level of ACE activity was significantly higher in the DD genotype (91.1 ± 23.18) than those in ID (60.6 ± 22.8) and in II genotypes (36.8 ± 6.9). There was a significant difference in genotype distribution between the two groups (P < 0.001). New normal ranges of serum ACE level were determined for each genotype. Moreover, we found test sensitivity to be 62.3%. Serum ACE activity was significantly associated with ACE (I/D) gene polymorphism. |
| first_indexed | 2024-04-12T20:13:51Z |
| format | Article |
| id | doaj.art-86f284cc8bf84be6af77ff37ebf2b6a5 |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-04-12T20:13:51Z |
| publishDate | 2008-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-86f284cc8bf84be6af77ff37ebf2b6a52022-12-22T03:18:10ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942008-08-01464POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTSA Nikzamir0M Nakhjavani1T Golmohammadi2L Dibai3R Saffary4Department of Biochemistry, School of Medicine, Ahwaz Jondi Shapour University of Medical Sciences, Endocrinology and Metabolism Research Center (EMRC), Vali-e-Asr Hospital, Tehran University of MedicDepartment of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Endocrinology and Metabolism Research Center (EMRC), Vali-e-Asr Hospital, Tehran University of MedicEndocrinology and Metabolism Research Center (EMRC), Vali-e-Asr Hospital, Tehran University of MedicDiabetes mellitus is a multifactorial disease. It has recently been shown that an insertion (I)/deletion (D) polymorphism exists in the angiotensin-converting enzyme (ACE) gene that can affect the serum ACE level. There are three genotypes: DD, DI, and II, with the ACE level being highest in DD, intermediate in DI, and lowest in II. In the present investigation, 170 patients with type 2 diabetes mellitus (T2DM) and 144 control subjects were studied. The ACE I/D polymorphism was determined by polymerase chain reaction (PCR) utilizing specific primers. ACE activity was determined spectrophotometrically. Distribution of ACE gene (I/D) polymorphism and allele frequencies in patients with T2DM were significantly different from those in control (P < 0.001); D allele frequency was 51% in T2DM vs. 48% in controls. The level of ACE activity was significantly higher in the DD genotype (91.1 ± 23.18) than those in ID (60.6 ± 22.8) and in II genotypes (36.8 ± 6.9). There was a significant difference in genotype distribution between the two groups (P < 0.001). New normal ranges of serum ACE level were determined for each genotype. Moreover, we found test sensitivity to be 62.3%. Serum ACE activity was significantly associated with ACE (I/D) gene polymorphism.https://acta.tums.ac.ir/index.php/acta/article/view/3482 |
| spellingShingle | A Nikzamir M Nakhjavani T Golmohammadi L Dibai R Saffary POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS Acta Medica Iranica |
| title | POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS |
| title_full | POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS |
| title_fullStr | POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS |
| title_full_unstemmed | POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS |
| title_short | POLYMORPHISM IN THE ANGIOTENSIN-CONVERTING ENZYME (ACE) GENE AND ACE ACTIVITY IN TYPE 2 DIABETIC PATIENTS |
| title_sort | polymorphism in the angiotensin converting enzyme ace gene and ace activity in type 2 diabetic patients |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/3482 |
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