Electrochemical biosensor-based devices for continuous phenols monitoring in environmental matrices
A flow system method for continuous determination of phenolic compounds in environmental matrices was employed using a dialysis membrane sampler and laccase- and tyrosinase-based biosensors as detector. The biosensors response to different phenolic compounds was investigated. The laccase-based biose...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2002-01-01
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| Series: | Journal of the Brazilian Chemical Society |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532002000400008 |
| _version_ | 1811194634547757056 |
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| author | Freire Renato S. Durán Nelson Kubota Lauro T. |
| author_facet | Freire Renato S. Durán Nelson Kubota Lauro T. |
| author_sort | Freire Renato S. |
| collection | DOAJ |
| description | A flow system method for continuous determination of phenolic compounds in environmental matrices was employed using a dialysis membrane sampler and laccase- and tyrosinase-based biosensors as detector. The biosensors response to different phenolic compounds was investigated. The laccase-based biosensor showed high sensitivity to guaiacol and chloroguaiacol, while the tyrosinase-based biosensor was more sensitive to phenol and chlorophenol. Both of the biosensors presented highly selective measurements of micromolar concentration of these compounds. Detection limits around 1.1 x 10-7 mol L-1 for guaiacol and 1.9 x 10-7 mol L-1 for chloroguaiacol could be estimated for the laccase-based biosensor. Using the tyrosinase-based biosensor detection limits of 1.5 x 10-7 mol L-1 for phenol and 9.0 x 10-8 mol L-1 for chlorophenol were observed. The proposed flow method presented a linear response range between 1.0 mumol L-1 and 100.0 mumol L-1, in the optimized operational conditions (laccase-based biosensor: pH 5.0 and 0 mV vs Ag/AgCl as working potential; tyrosinase-based biosensor: pH 5.5 and 50 mV vs Ag/AgCl as working potential). Real paper mill effluent sample was analyzed by this system and by the reference colorimetric method and the results were discussed. |
| first_indexed | 2024-04-12T00:30:01Z |
| format | Article |
| id | doaj.art-86f450306da74189aeb403ce19ba211a |
| institution | Directory Open Access Journal |
| issn | 0103-5053 |
| language | English |
| last_indexed | 2024-04-12T00:30:01Z |
| publishDate | 2002-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Journal of the Brazilian Chemical Society |
| spelling | doaj.art-86f450306da74189aeb403ce19ba211a2022-12-22T03:55:22ZengSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society0103-50532002-01-01134456462Electrochemical biosensor-based devices for continuous phenols monitoring in environmental matricesFreire Renato S.Durán NelsonKubota Lauro T.A flow system method for continuous determination of phenolic compounds in environmental matrices was employed using a dialysis membrane sampler and laccase- and tyrosinase-based biosensors as detector. The biosensors response to different phenolic compounds was investigated. The laccase-based biosensor showed high sensitivity to guaiacol and chloroguaiacol, while the tyrosinase-based biosensor was more sensitive to phenol and chlorophenol. Both of the biosensors presented highly selective measurements of micromolar concentration of these compounds. Detection limits around 1.1 x 10-7 mol L-1 for guaiacol and 1.9 x 10-7 mol L-1 for chloroguaiacol could be estimated for the laccase-based biosensor. Using the tyrosinase-based biosensor detection limits of 1.5 x 10-7 mol L-1 for phenol and 9.0 x 10-8 mol L-1 for chlorophenol were observed. The proposed flow method presented a linear response range between 1.0 mumol L-1 and 100.0 mumol L-1, in the optimized operational conditions (laccase-based biosensor: pH 5.0 and 0 mV vs Ag/AgCl as working potential; tyrosinase-based biosensor: pH 5.5 and 50 mV vs Ag/AgCl as working potential). Real paper mill effluent sample was analyzed by this system and by the reference colorimetric method and the results were discussed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532002000400008amperometric biosensorscontinuous monitoringenvironmental samplesphenolic compounds |
| spellingShingle | Freire Renato S. Durán Nelson Kubota Lauro T. Electrochemical biosensor-based devices for continuous phenols monitoring in environmental matrices Journal of the Brazilian Chemical Society amperometric biosensors continuous monitoring environmental samples phenolic compounds |
| title | Electrochemical biosensor-based devices for continuous phenols monitoring in environmental matrices |
| title_full | Electrochemical biosensor-based devices for continuous phenols monitoring in environmental matrices |
| title_fullStr | Electrochemical biosensor-based devices for continuous phenols monitoring in environmental matrices |
| title_full_unstemmed | Electrochemical biosensor-based devices for continuous phenols monitoring in environmental matrices |
| title_short | Electrochemical biosensor-based devices for continuous phenols monitoring in environmental matrices |
| title_sort | electrochemical biosensor based devices for continuous phenols monitoring in environmental matrices |
| topic | amperometric biosensors continuous monitoring environmental samples phenolic compounds |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532002000400008 |
| work_keys_str_mv | AT freirerenatos electrochemicalbiosensorbaseddevicesforcontinuousphenolsmonitoringinenvironmentalmatrices AT durannelson electrochemicalbiosensorbaseddevicesforcontinuousphenolsmonitoringinenvironmentalmatrices AT kubotalaurot electrochemicalbiosensorbaseddevicesforcontinuousphenolsmonitoringinenvironmentalmatrices |