Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato Plants
Glutathione transferases (GSTs) are one of the most versatile multigenic enzyme superfamilies. In our experiments, the involvement of the genotype-specific induction of <i>GST</i> genes and glutathione- or redox-related genes in pathways regulating salt-stress tolerance was examined in t...
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MDPI AG
2023-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/9/1682 |
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| author | Edit Horváth Kitti Kulman Bernát Tompa Ádám Barnabás Hajnal Alina Pelsőczi Krisztina Bela Ágnes Gallé Jolán Csiszár |
| author_facet | Edit Horváth Kitti Kulman Bernát Tompa Ádám Barnabás Hajnal Alina Pelsőczi Krisztina Bela Ágnes Gallé Jolán Csiszár |
| author_sort | Edit Horváth |
| collection | DOAJ |
| description | Glutathione transferases (GSTs) are one of the most versatile multigenic enzyme superfamilies. In our experiments, the involvement of the genotype-specific induction of <i>GST</i> genes and glutathione- or redox-related genes in pathways regulating salt-stress tolerance was examined in tomato cultivars (<i>Solanum lycopersicum</i> Moneymaker, Mobil, and Elán F1). The growth of the Mobil plants was adversely affected during salt stress (100 mM of NaCl), which might be the result of lowered glutathione and ascorbate levels, a more positive glutathione redox potential (<i>E<sub>GSH</sub></i>), and reduced glutathione reductase (GR) and GST activities. In contrast, the Moneymaker and Elán F1 cultivars were able to restore their growth and exhibited higher GR and inducible GST activities, as well as elevated, non-enzymatic antioxidant levels, indicating their enhanced salt tolerance. Furthermore, the expression patterns of <i>GR</i>, selected <i>GST</i>, and transcription factor genes differed significantly among the three cultivars, highlighting the distinct regulatory mechanisms of the tomato genotypes during salt stress. The correlations between <i>E<sub>GSH</sub></i> and gene expression data revealed several robust, cultivar-specific associations, underscoring the complexity of the stress response mechanism in tomatoes. Our results support the cultivar-specific roles of distinct <i>GST</i> genes during the salt-stress response, which, along with <i>WRKY3</i>, <i>WRKY72</i>, <i>DREB1</i>, and <i>DREB2</i>, are important players in shaping the redox status and the development of a more efficient stress tolerance in tomatoes. |
| first_indexed | 2024-03-10T23:06:36Z |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T23:06:36Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-86f4a19c9d0c49d0b6ba20654df648772023-11-19T09:18:56ZengMDPI AGAntioxidants2076-39212023-08-01129168210.3390/antiox12091682Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato PlantsEdit Horváth0Kitti Kulman1Bernát Tompa2Ádám Barnabás Hajnal3Alina Pelsőczi4Krisztina Bela5Ágnes Gallé6Jolán Csiszár7Department of Plant Biology, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Plant Biology, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Plant Biology, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Plant Biology, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Plant Biology, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Plant Biology, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Plant Biology, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, HungaryDepartment of Plant Biology, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, HungaryGlutathione transferases (GSTs) are one of the most versatile multigenic enzyme superfamilies. In our experiments, the involvement of the genotype-specific induction of <i>GST</i> genes and glutathione- or redox-related genes in pathways regulating salt-stress tolerance was examined in tomato cultivars (<i>Solanum lycopersicum</i> Moneymaker, Mobil, and Elán F1). The growth of the Mobil plants was adversely affected during salt stress (100 mM of NaCl), which might be the result of lowered glutathione and ascorbate levels, a more positive glutathione redox potential (<i>E<sub>GSH</sub></i>), and reduced glutathione reductase (GR) and GST activities. In contrast, the Moneymaker and Elán F1 cultivars were able to restore their growth and exhibited higher GR and inducible GST activities, as well as elevated, non-enzymatic antioxidant levels, indicating their enhanced salt tolerance. Furthermore, the expression patterns of <i>GR</i>, selected <i>GST</i>, and transcription factor genes differed significantly among the three cultivars, highlighting the distinct regulatory mechanisms of the tomato genotypes during salt stress. The correlations between <i>E<sub>GSH</sub></i> and gene expression data revealed several robust, cultivar-specific associations, underscoring the complexity of the stress response mechanism in tomatoes. Our results support the cultivar-specific roles of distinct <i>GST</i> genes during the salt-stress response, which, along with <i>WRKY3</i>, <i>WRKY72</i>, <i>DREB1</i>, and <i>DREB2</i>, are important players in shaping the redox status and the development of a more efficient stress tolerance in tomatoes.https://www.mdpi.com/2076-3921/12/9/1682glutathione transferasesredox homeostasissalt stress<i>Solanum lycopersicum</i> L.transcription regulation |
| spellingShingle | Edit Horváth Kitti Kulman Bernát Tompa Ádám Barnabás Hajnal Alina Pelsőczi Krisztina Bela Ágnes Gallé Jolán Csiszár Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato Plants Antioxidants glutathione transferases redox homeostasis salt stress <i>Solanum lycopersicum</i> L. transcription regulation |
| title | Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato Plants |
| title_full | Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato Plants |
| title_fullStr | Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato Plants |
| title_full_unstemmed | Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato Plants |
| title_short | Glutathione Transferases Are Involved in the Genotype-Specific Salt-Stress Response of Tomato Plants |
| title_sort | glutathione transferases are involved in the genotype specific salt stress response of tomato plants |
| topic | glutathione transferases redox homeostasis salt stress <i>Solanum lycopersicum</i> L. transcription regulation |
| url | https://www.mdpi.com/2076-3921/12/9/1682 |
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