Physico-chemical characteristics and cytotoxicity evaluation of CuO and TiO2 nanoparticles biosynthesized using extracts of Mucuna pruriens utilis seeds
The green synthesis approach to nanoparticles has been widely received as an alternative to the conventional methods, specifically for applications in areas such as biology, agriculture and medicine, where toxicity is of great concern. In this study, copper oxide (CuO) and titanium oxide (TiO2) nano...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402201475X |
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| author | Nozipho P. Gamedze Doctor Mziwenkosi Nhlanhla Mthiyane Olubukola Oluranti Babalola Moganavelli Singh Damian C. Onwudiwe |
| author_facet | Nozipho P. Gamedze Doctor Mziwenkosi Nhlanhla Mthiyane Olubukola Oluranti Babalola Moganavelli Singh Damian C. Onwudiwe |
| author_sort | Nozipho P. Gamedze |
| collection | DOAJ |
| description | The green synthesis approach to nanoparticles has been widely received as an alternative to the conventional methods, specifically for applications in areas such as biology, agriculture and medicine, where toxicity is of great concern. In this study, copper oxide (CuO) and titanium oxide (TiO2) nanoparticles (NPs) were synthesized using an aqueous extract of Mucuna pruriens utilis seed. The morphology and structural characterization of the NPs were achieved by using scanning and transmission electron microscopy (SEM and TEM), and X-ray diffraction (XRD) measurement, while the elemental composition was studied using electron diffraction X-ray spectroscopy (EDS). A monoclinic phase of CuO and anatase phases of TiO2 with high crystallinity were confirmed from the diffraction patterns of the XRD. Both TEM and SEM micrographs of the CuO confirmed short rod-shaped nanostructure, while spherical morphologies were obtained for the TiO2 NPs. The EDS study indicated that the composition of the samples conformed with the identified products in the XRD and attest to the purity of the NPs. The nanoparticles exhibited a dose-dependent profile in MTT cytotoxicity assay with some cell specificity. However, the anticancer potential of these NPs was still lower than that of the standard anticancer drug, 5-fluorouracil. |
| first_indexed | 2024-04-14T05:32:57Z |
| format | Article |
| id | doaj.art-86f4c13db88e44b893a025f4bfda2633 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-14T05:32:57Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-86f4c13db88e44b893a025f4bfda26332022-12-22T02:09:44ZengElsevierHeliyon2405-84402022-08-0188e10187Physico-chemical characteristics and cytotoxicity evaluation of CuO and TiO2 nanoparticles biosynthesized using extracts of Mucuna pruriens utilis seedsNozipho P. Gamedze0Doctor Mziwenkosi Nhlanhla Mthiyane1Olubukola Oluranti Babalola2Moganavelli Singh3Damian C. Onwudiwe4Department of Animal Science, School of Agricultural Sciences, Faculty of Natural and Agricultural Science, North-West University (Mafikeng Campus), Private Bag X2046, Mmabatho, South Africa; Food Security and Safety Focus area, Faculty of Natural and Agricultural Science, North-West University, Mmabatho 2735, South AfricaDepartment of Animal Science, School of Agricultural Sciences, Faculty of Natural and Agricultural Science, North-West University (Mafikeng Campus), Private Bag X2046, Mmabatho, South Africa; Food Security and Safety Focus area, Faculty of Natural and Agricultural Science, North-West University, Mmabatho 2735, South AfricaFood Security and Safety Focus area, Faculty of Natural and Agricultural Science, North-West University, Mmabatho 2735, South AfricaNano-Gene and Drug Delivery Laboratory, Department of Biochemistry, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South AfricaMaterial Science Innovation and Modelling (MaSIM) Research Focus Area, Faculty of Faculty of Natural and Agricultural Science, North-West University (Mafikeng Campus), Private Bag X2046, Mmabatho, South Africa; Department of Chemistry, School of Physical and Chemical Sciences, Faculty of Natural and Agricultural Science, North-West University (Mafikeng Campus), Private Bag X2046, Mmabatho, South Africa; Corresponding author.The green synthesis approach to nanoparticles has been widely received as an alternative to the conventional methods, specifically for applications in areas such as biology, agriculture and medicine, where toxicity is of great concern. In this study, copper oxide (CuO) and titanium oxide (TiO2) nanoparticles (NPs) were synthesized using an aqueous extract of Mucuna pruriens utilis seed. The morphology and structural characterization of the NPs were achieved by using scanning and transmission electron microscopy (SEM and TEM), and X-ray diffraction (XRD) measurement, while the elemental composition was studied using electron diffraction X-ray spectroscopy (EDS). A monoclinic phase of CuO and anatase phases of TiO2 with high crystallinity were confirmed from the diffraction patterns of the XRD. Both TEM and SEM micrographs of the CuO confirmed short rod-shaped nanostructure, while spherical morphologies were obtained for the TiO2 NPs. The EDS study indicated that the composition of the samples conformed with the identified products in the XRD and attest to the purity of the NPs. The nanoparticles exhibited a dose-dependent profile in MTT cytotoxicity assay with some cell specificity. However, the anticancer potential of these NPs was still lower than that of the standard anticancer drug, 5-fluorouracil.http://www.sciencedirect.com/science/article/pii/S240584402201475XAgricultural nanotechnologyCopper oxideTitanium oxideBiosynthesisCytotoxicityMucuna pruriens utilis |
| spellingShingle | Nozipho P. Gamedze Doctor Mziwenkosi Nhlanhla Mthiyane Olubukola Oluranti Babalola Moganavelli Singh Damian C. Onwudiwe Physico-chemical characteristics and cytotoxicity evaluation of CuO and TiO2 nanoparticles biosynthesized using extracts of Mucuna pruriens utilis seeds Heliyon Agricultural nanotechnology Copper oxide Titanium oxide Biosynthesis Cytotoxicity Mucuna pruriens utilis |
| title | Physico-chemical characteristics and cytotoxicity evaluation of CuO and TiO2 nanoparticles biosynthesized using extracts of Mucuna pruriens utilis seeds |
| title_full | Physico-chemical characteristics and cytotoxicity evaluation of CuO and TiO2 nanoparticles biosynthesized using extracts of Mucuna pruriens utilis seeds |
| title_fullStr | Physico-chemical characteristics and cytotoxicity evaluation of CuO and TiO2 nanoparticles biosynthesized using extracts of Mucuna pruriens utilis seeds |
| title_full_unstemmed | Physico-chemical characteristics and cytotoxicity evaluation of CuO and TiO2 nanoparticles biosynthesized using extracts of Mucuna pruriens utilis seeds |
| title_short | Physico-chemical characteristics and cytotoxicity evaluation of CuO and TiO2 nanoparticles biosynthesized using extracts of Mucuna pruriens utilis seeds |
| title_sort | physico chemical characteristics and cytotoxicity evaluation of cuo and tio2 nanoparticles biosynthesized using extracts of mucuna pruriens utilis seeds |
| topic | Agricultural nanotechnology Copper oxide Titanium oxide Biosynthesis Cytotoxicity Mucuna pruriens utilis |
| url | http://www.sciencedirect.com/science/article/pii/S240584402201475X |
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