Polymorphisms of the cryptochrome 2 and mitoguardin 2 genes are associated with the variation of lipid-related traits in Duroc pigs
Abstract The genetic factors determining the phenotypic variation of porcine fatness phenotypes are still largely unknown. We investigated whether the polymorphism of eight genes (MIGA2, CRY2, NPAS2, CIART, ARNTL2, PER1, PER2 and PCK1), which display differential expression in the skeletal muscle of...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2019-06-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-019-45108-z |
| _version_ | 1818996075980128256 |
|---|---|
| author | Emilio Mármol-Sánchez Raquel Quintanilla Taina F. Cardoso Jordi Jordana Vidal Marcel Amills |
| author_facet | Emilio Mármol-Sánchez Raquel Quintanilla Taina F. Cardoso Jordi Jordana Vidal Marcel Amills |
| author_sort | Emilio Mármol-Sánchez |
| collection | DOAJ |
| description | Abstract The genetic factors determining the phenotypic variation of porcine fatness phenotypes are still largely unknown. We investigated whether the polymorphism of eight genes (MIGA2, CRY2, NPAS2, CIART, ARNTL2, PER1, PER2 and PCK1), which display differential expression in the skeletal muscle of fasted and fed sows, is associated with the variation of lipid and mRNA expression phenotypes in Duroc pigs. The performance of an association analysis with the GEMMA software demonstrated that the rs330779504 SNP in the MIGA2 gene is associated with LDL concentration at 190 days (LDL2, corrected P-value = 0.057). Moreover, the rs320439526 SNP of the CRY2 gene displayed a significant association with stearic acid content in the longissimus dorsi muscle (LD C18:0, corrected P-value = 0.015). Both SNPs were also associated with the mRNA levels of the corresponding genes in the gluteus medius skeletal muscle. From a biological perspective these results are meaningful because MIGA2 protein plays an essential role in mitochondrial fusion, a process tightly connected with the energy status of the cell, while CRY2 is a fundamental component of the circadian clock. However, inclusion of these two SNPs in chromosome-wide association analyses demonstrated that they are not located at the peaks of significance for the two traits under study (LDL2 for rs330779504 and LD C18:0 for rs320439526), thus implying that these two SNPs do not have causal effects. |
| first_indexed | 2024-12-20T21:23:59Z |
| format | Article |
| id | doaj.art-86f5d99e83a54a0a8eddb692f1bcc6af |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:23:59Z |
| publishDate | 2019-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-86f5d99e83a54a0a8eddb692f1bcc6af2022-12-21T19:26:12ZengNature PortfolioScientific Reports2045-23222019-06-01911910.1038/s41598-019-45108-zPolymorphisms of the cryptochrome 2 and mitoguardin 2 genes are associated with the variation of lipid-related traits in Duroc pigsEmilio Mármol-Sánchez0Raquel Quintanilla1Taina F. Cardoso2Jordi Jordana Vidal3Marcel Amills4Department of Animal Genetics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus de la Universitat Autònoma de BarcelonaAnimal Breeding and Genetics Programme, Institute for Research and Technology in Food and Agriculture (IRTA), Torre MarimonDepartment of Animal Genetics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus de la Universitat Autònoma de BarcelonaDepartament de Ciència Animal i dels Aliments, Universitat Autònoma de BarcelonaDepartment of Animal Genetics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus de la Universitat Autònoma de BarcelonaAbstract The genetic factors determining the phenotypic variation of porcine fatness phenotypes are still largely unknown. We investigated whether the polymorphism of eight genes (MIGA2, CRY2, NPAS2, CIART, ARNTL2, PER1, PER2 and PCK1), which display differential expression in the skeletal muscle of fasted and fed sows, is associated with the variation of lipid and mRNA expression phenotypes in Duroc pigs. The performance of an association analysis with the GEMMA software demonstrated that the rs330779504 SNP in the MIGA2 gene is associated with LDL concentration at 190 days (LDL2, corrected P-value = 0.057). Moreover, the rs320439526 SNP of the CRY2 gene displayed a significant association with stearic acid content in the longissimus dorsi muscle (LD C18:0, corrected P-value = 0.015). Both SNPs were also associated with the mRNA levels of the corresponding genes in the gluteus medius skeletal muscle. From a biological perspective these results are meaningful because MIGA2 protein plays an essential role in mitochondrial fusion, a process tightly connected with the energy status of the cell, while CRY2 is a fundamental component of the circadian clock. However, inclusion of these two SNPs in chromosome-wide association analyses demonstrated that they are not located at the peaks of significance for the two traits under study (LDL2 for rs330779504 and LD C18:0 for rs320439526), thus implying that these two SNPs do not have causal effects.https://doi.org/10.1038/s41598-019-45108-z |
| spellingShingle | Emilio Mármol-Sánchez Raquel Quintanilla Taina F. Cardoso Jordi Jordana Vidal Marcel Amills Polymorphisms of the cryptochrome 2 and mitoguardin 2 genes are associated with the variation of lipid-related traits in Duroc pigs Scientific Reports |
| title | Polymorphisms of the cryptochrome 2 and mitoguardin 2 genes are associated with the variation of lipid-related traits in Duroc pigs |
| title_full | Polymorphisms of the cryptochrome 2 and mitoguardin 2 genes are associated with the variation of lipid-related traits in Duroc pigs |
| title_fullStr | Polymorphisms of the cryptochrome 2 and mitoguardin 2 genes are associated with the variation of lipid-related traits in Duroc pigs |
| title_full_unstemmed | Polymorphisms of the cryptochrome 2 and mitoguardin 2 genes are associated with the variation of lipid-related traits in Duroc pigs |
| title_short | Polymorphisms of the cryptochrome 2 and mitoguardin 2 genes are associated with the variation of lipid-related traits in Duroc pigs |
| title_sort | polymorphisms of the cryptochrome 2 and mitoguardin 2 genes are associated with the variation of lipid related traits in duroc pigs |
| url | https://doi.org/10.1038/s41598-019-45108-z |
| work_keys_str_mv | AT emiliomarmolsanchez polymorphismsofthecryptochrome2andmitoguardin2genesareassociatedwiththevariationoflipidrelatedtraitsindurocpigs AT raquelquintanilla polymorphismsofthecryptochrome2andmitoguardin2genesareassociatedwiththevariationoflipidrelatedtraitsindurocpigs AT tainafcardoso polymorphismsofthecryptochrome2andmitoguardin2genesareassociatedwiththevariationoflipidrelatedtraitsindurocpigs AT jordijordanavidal polymorphismsofthecryptochrome2andmitoguardin2genesareassociatedwiththevariationoflipidrelatedtraitsindurocpigs AT marcelamills polymorphismsofthecryptochrome2andmitoguardin2genesareassociatedwiththevariationoflipidrelatedtraitsindurocpigs |