MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer

Background: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 e...

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Main Authors: Hai-Bin Liang, Yang Cao, Qiang Ma, Yi-Jun Shu, Zheng Wang, Fei Zhang, Yuan-Yuan Ye, Huai-Feng Li, Shan-Shan Xiang, Xiao-Ling Song, Yi Xu, Yi-Chi Zhang, Run-Fa Bao, Rui-Yan Yuan, Yi-Jian Zhang, Yun-Ping Hu, Lin Jiang, Mao-Lan Li, Xu-An Wang, Xiang-Song Wu, Wen-Guang Wu, Shuai Zhao, Yong Fand, Xiao-Peng Cui, Yun-Shu Lu, Jian Zhou, Lei Zheng, Wei Gong, Ying-Bin Liu
Format: Article
Language:English
Published: Cell Physiol Biochem Press GmbH & Co KG 2017-04-01
Series:Cellular Physiology and Biochemistry
Subjects:
Online Access:http://www.karger.com/Article/FullText/475454
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author Hai-Bin Liang
Yang Cao
Qiang Ma
Yi-Jun Shu
Zheng Wang
Fei Zhang
Yuan-Yuan Ye
Huai-Feng Li
Shan-Shan Xiang
Xiao-Ling Song
Yi Xu
Yi-Chi Zhang
Run-Fa Bao
Rui-Yan Yuan
Yi-Jian Zhang
Yun-Ping Hu
Lin Jiang
Mao-Lan Li
Xu-An Wang
Xiang-Song Wu
Wen-Guang Wu
Shuai Zhao
Yong Fand
Xiao-Peng Cui
Yun-Shu Lu
Jian Zhou
Lei Zheng
Wei Gong
Ying-Bin Liu
author_facet Hai-Bin Liang
Yang Cao
Qiang Ma
Yi-Jun Shu
Zheng Wang
Fei Zhang
Yuan-Yuan Ye
Huai-Feng Li
Shan-Shan Xiang
Xiao-Ling Song
Yi Xu
Yi-Chi Zhang
Run-Fa Bao
Rui-Yan Yuan
Yi-Jian Zhang
Yun-Ping Hu
Lin Jiang
Mao-Lan Li
Xu-An Wang
Xiang-Song Wu
Wen-Guang Wu
Shuai Zhao
Yong Fand
Xiao-Peng Cui
Yun-Shu Lu
Jian Zhou
Lei Zheng
Wei Gong
Ying-Bin Liu
author_sort Hai-Bin Liang
collection DOAJ
description Background: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 expression levels were measured in GBC and cholecystitis tissue specimens using quantitative real-time PCR (qRT-PCR) and immunohistochemical (IHC) assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) retention assay, flow cytometry analysis, western blot, and a xenograft model of GBC cells in nude mice. Results: MYBL2 expression was increased in GBC tissues and associated with histological differentiation, tumour invasion, clinical stage and unfavourable overall survival in GBC patients. The downregulation of MYBL2 expression resulted in the inhibition of GBC cell proliferation, and DNA replication in vitro, and the growth of xenografted tumours in nude mice. Conversely, MYBL2 overexpression resulted in the opposite effects. Conclusions: MYBL2 overexpression promotes GBC cell proliferation through the regulation of the cell cycle at the S and G2/M phase transitions. Thus, MYBL2 could serve as a potential prognostic and therapeutic biomarker in GBC patients.
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spelling doaj.art-86f84e46e1bf4cf5886bfd7baae391702022-12-22T01:13:35ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-04-014152117213110.1159/000475454475454MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder CancerHai-Bin LiangYang CaoQiang MaYi-Jun ShuZheng WangFei ZhangYuan-Yuan YeHuai-Feng LiShan-Shan XiangXiao-Ling SongYi XuYi-Chi ZhangRun-Fa BaoRui-Yan YuanYi-Jian ZhangYun-Ping HuLin JiangMao-Lan LiXu-An WangXiang-Song WuWen-Guang WuShuai ZhaoYong FandXiao-Peng CuiYun-Shu LuJian ZhouLei ZhengWei GongYing-Bin LiuBackground: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 expression levels were measured in GBC and cholecystitis tissue specimens using quantitative real-time PCR (qRT-PCR) and immunohistochemical (IHC) assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) retention assay, flow cytometry analysis, western blot, and a xenograft model of GBC cells in nude mice. Results: MYBL2 expression was increased in GBC tissues and associated with histological differentiation, tumour invasion, clinical stage and unfavourable overall survival in GBC patients. The downregulation of MYBL2 expression resulted in the inhibition of GBC cell proliferation, and DNA replication in vitro, and the growth of xenografted tumours in nude mice. Conversely, MYBL2 overexpression resulted in the opposite effects. Conclusions: MYBL2 overexpression promotes GBC cell proliferation through the regulation of the cell cycle at the S and G2/M phase transitions. Thus, MYBL2 could serve as a potential prognostic and therapeutic biomarker in GBC patients.http://www.karger.com/Article/FullText/475454Cell cycleS phaseMYBL2Gallbladder cancerCell proliferationG2/M phase
spellingShingle Hai-Bin Liang
Yang Cao
Qiang Ma
Yi-Jun Shu
Zheng Wang
Fei Zhang
Yuan-Yuan Ye
Huai-Feng Li
Shan-Shan Xiang
Xiao-Ling Song
Yi Xu
Yi-Chi Zhang
Run-Fa Bao
Rui-Yan Yuan
Yi-Jian Zhang
Yun-Ping Hu
Lin Jiang
Mao-Lan Li
Xu-An Wang
Xiang-Song Wu
Wen-Guang Wu
Shuai Zhao
Yong Fand
Xiao-Peng Cui
Yun-Shu Lu
Jian Zhou
Lei Zheng
Wei Gong
Ying-Bin Liu
MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer
Cellular Physiology and Biochemistry
Cell cycle
S phase
MYBL2
Gallbladder cancer
Cell proliferation
G2/M phase
title MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer
title_full MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer
title_fullStr MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer
title_full_unstemmed MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer
title_short MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer
title_sort mybl2 is a potential prognostic marker that promotes cell proliferation in gallbladder cancer
topic Cell cycle
S phase
MYBL2
Gallbladder cancer
Cell proliferation
G2/M phase
url http://www.karger.com/Article/FullText/475454
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