MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer
Background: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 e...
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Format: | Article |
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Cell Physiol Biochem Press GmbH & Co KG
2017-04-01
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Series: | Cellular Physiology and Biochemistry |
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Online Access: | http://www.karger.com/Article/FullText/475454 |
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author | Hai-Bin Liang Yang Cao Qiang Ma Yi-Jun Shu Zheng Wang Fei Zhang Yuan-Yuan Ye Huai-Feng Li Shan-Shan Xiang Xiao-Ling Song Yi Xu Yi-Chi Zhang Run-Fa Bao Rui-Yan Yuan Yi-Jian Zhang Yun-Ping Hu Lin Jiang Mao-Lan Li Xu-An Wang Xiang-Song Wu Wen-Guang Wu Shuai Zhao Yong Fand Xiao-Peng Cui Yun-Shu Lu Jian Zhou Lei Zheng Wei Gong Ying-Bin Liu |
author_facet | Hai-Bin Liang Yang Cao Qiang Ma Yi-Jun Shu Zheng Wang Fei Zhang Yuan-Yuan Ye Huai-Feng Li Shan-Shan Xiang Xiao-Ling Song Yi Xu Yi-Chi Zhang Run-Fa Bao Rui-Yan Yuan Yi-Jian Zhang Yun-Ping Hu Lin Jiang Mao-Lan Li Xu-An Wang Xiang-Song Wu Wen-Guang Wu Shuai Zhao Yong Fand Xiao-Peng Cui Yun-Shu Lu Jian Zhou Lei Zheng Wei Gong Ying-Bin Liu |
author_sort | Hai-Bin Liang |
collection | DOAJ |
description | Background: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 expression levels were measured in GBC and cholecystitis tissue specimens using quantitative real-time PCR (qRT-PCR) and immunohistochemical (IHC) assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) retention assay, flow cytometry analysis, western blot, and a xenograft model of GBC cells in nude mice. Results: MYBL2 expression was increased in GBC tissues and associated with histological differentiation, tumour invasion, clinical stage and unfavourable overall survival in GBC patients. The downregulation of MYBL2 expression resulted in the inhibition of GBC cell proliferation, and DNA replication in vitro, and the growth of xenografted tumours in nude mice. Conversely, MYBL2 overexpression resulted in the opposite effects. Conclusions: MYBL2 overexpression promotes GBC cell proliferation through the regulation of the cell cycle at the S and G2/M phase transitions. Thus, MYBL2 could serve as a potential prognostic and therapeutic biomarker in GBC patients. |
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spelling | doaj.art-86f84e46e1bf4cf5886bfd7baae391702022-12-22T01:13:35ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-04-014152117213110.1159/000475454475454MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder CancerHai-Bin LiangYang CaoQiang MaYi-Jun ShuZheng WangFei ZhangYuan-Yuan YeHuai-Feng LiShan-Shan XiangXiao-Ling SongYi XuYi-Chi ZhangRun-Fa BaoRui-Yan YuanYi-Jian ZhangYun-Ping HuLin JiangMao-Lan LiXu-An WangXiang-Song WuWen-Guang WuShuai ZhaoYong FandXiao-Peng CuiYun-Shu LuJian ZhouLei ZhengWei GongYing-Bin LiuBackground: Gallbladder cancer (GBC) is an aggressive and highly lethal biliary tract malignancy, with extremely poor prognosis. In the present study, we analyzed the potential involvement of MYBL2, a member of the Myb transcription factor family, in the carcinogenesis of human GBC. Methods: MYBL2 expression levels were measured in GBC and cholecystitis tissue specimens using quantitative real-time PCR (qRT-PCR) and immunohistochemical (IHC) assays. The effects of MYBL2 on cell proliferation and DNA synthesis were evaluated using Cell Counting Kit-8 assay (CCK-8), colony formation, and 5-ethynyl-2'-deoxyuridine (EdU) retention assay, flow cytometry analysis, western blot, and a xenograft model of GBC cells in nude mice. Results: MYBL2 expression was increased in GBC tissues and associated with histological differentiation, tumour invasion, clinical stage and unfavourable overall survival in GBC patients. The downregulation of MYBL2 expression resulted in the inhibition of GBC cell proliferation, and DNA replication in vitro, and the growth of xenografted tumours in nude mice. Conversely, MYBL2 overexpression resulted in the opposite effects. Conclusions: MYBL2 overexpression promotes GBC cell proliferation through the regulation of the cell cycle at the S and G2/M phase transitions. Thus, MYBL2 could serve as a potential prognostic and therapeutic biomarker in GBC patients.http://www.karger.com/Article/FullText/475454Cell cycleS phaseMYBL2Gallbladder cancerCell proliferationG2/M phase |
spellingShingle | Hai-Bin Liang Yang Cao Qiang Ma Yi-Jun Shu Zheng Wang Fei Zhang Yuan-Yuan Ye Huai-Feng Li Shan-Shan Xiang Xiao-Ling Song Yi Xu Yi-Chi Zhang Run-Fa Bao Rui-Yan Yuan Yi-Jian Zhang Yun-Ping Hu Lin Jiang Mao-Lan Li Xu-An Wang Xiang-Song Wu Wen-Guang Wu Shuai Zhao Yong Fand Xiao-Peng Cui Yun-Shu Lu Jian Zhou Lei Zheng Wei Gong Ying-Bin Liu MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer Cellular Physiology and Biochemistry Cell cycle S phase MYBL2 Gallbladder cancer Cell proliferation G2/M phase |
title | MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer |
title_full | MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer |
title_fullStr | MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer |
title_full_unstemmed | MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer |
title_short | MYBL2 is a Potential Prognostic Marker that Promotes Cell Proliferation in Gallbladder Cancer |
title_sort | mybl2 is a potential prognostic marker that promotes cell proliferation in gallbladder cancer |
topic | Cell cycle S phase MYBL2 Gallbladder cancer Cell proliferation G2/M phase |
url | http://www.karger.com/Article/FullText/475454 |
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