Comparison of Two Methods for the Isolation of Genomic DNA from Cyathostomin Adult Parasites
Cyathostomins are the most common and important group of large intestine nematodes, infecting horses worldwide. The current control strategy is associated with the development of anthelmintic resistance, which has been reported worldwide. Therefore, experiments with this family of parasites have bec...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Istanbul University
2017-07-01
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| Series: | İstanbul Üniversitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | http://dergipark.gov.tr/iuvfd/issue/26805/320203 |
| _version_ | 1797910799263465472 |
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| author | Juliana Bana ISHII Amilcar ARENAL Yanise CHICOY Marcelo Beltrão MOLENTO |
| author_facet | Juliana Bana ISHII Amilcar ARENAL Yanise CHICOY Marcelo Beltrão MOLENTO |
| author_sort | Juliana Bana ISHII |
| collection | DOAJ |
| description | Cyathostomins are the most common and important group of large intestine nematodes, infecting horses worldwide. The current control strategy is associated with the development of anthelmintic resistance, which has been reported worldwide. Therefore, experiments with this family of parasites have become progressively important to provide their monitoring and control strategies. The aim of the present study was to propose a faster and more economic assay for isolation of genomic DNA from the adult stage of Cyathostomin parasites than reported. Adult parasites were collected from a single horse from a farm in São José dos Pinhais, PR, Brazil, and were identified. Genomic DNA was isolated from ten individual female adult parasites using a standardized procedure developed. Then, extraction from ten individual female was carried out by another DNA extraction method. DNA concentration from both methods were measured and compared. We obtained a good DNA quality with this standardized procedure. As a result of this analysis, we propose a modified phenol-chloroform method, which will contribute to assays that require DNA extraction from adult worms for genomic DNA sequences of cyathostomin, or species-specific identification. |
| first_indexed | 2024-04-10T11:30:03Z |
| format | Article |
| id | doaj.art-86fbf9a1fcd843009942afe0e1960df1 |
| institution | Directory Open Access Journal |
| issn | 0250-2836 0250-2836 |
| language | English |
| last_indexed | 2024-04-10T11:30:03Z |
| publishDate | 2017-07-01 |
| publisher | Istanbul University |
| record_format | Article |
| series | İstanbul Üniversitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-86fbf9a1fcd843009942afe0e1960df12023-02-15T16:18:10ZengIstanbul Universityİstanbul Üniversitesi Veteriner Fakültesi Dergisi0250-28360250-28362017-07-0143214614810.16988/iuvfd.320203Comparison of Two Methods for the Isolation of Genomic DNA from Cyathostomin Adult ParasitesJuliana Bana ISHII0Amilcar ARENAL1Yanise CHICOY2Marcelo Beltrão MOLENTO3Laboratory of Parasitic Diseases, Federal University of Parana. Curitiba, PR, BrazilLaboratory of Biochemistry, University of Camaguey. Camaguey, CubaLaboratory of Biochemistry, University of Camaguey. Camaguey, CubaLaboratory of Parasitic Diseases, Federal University of Parana. Curitiba, PR, Brazil and National Institute of Science and Technology, INCT- Pecuaria. Belo Horizonte, MG. Brazil Cyathostomins are the most common and important group of large intestine nematodes, infecting horses worldwide. The current control strategy is associated with the development of anthelmintic resistance, which has been reported worldwide. Therefore, experiments with this family of parasites have become progressively important to provide their monitoring and control strategies. The aim of the present study was to propose a faster and more economic assay for isolation of genomic DNA from the adult stage of Cyathostomin parasites than reported. Adult parasites were collected from a single horse from a farm in São José dos Pinhais, PR, Brazil, and were identified. Genomic DNA was isolated from ten individual female adult parasites using a standardized procedure developed. Then, extraction from ten individual female was carried out by another DNA extraction method. DNA concentration from both methods were measured and compared. We obtained a good DNA quality with this standardized procedure. As a result of this analysis, we propose a modified phenol-chloroform method, which will contribute to assays that require DNA extraction from adult worms for genomic DNA sequences of cyathostomin, or species-specific identification.http://dergipark.gov.tr/iuvfd/issue/26805/320203DNA extractiongenomic materialhorseStrongylida; |
| spellingShingle | Juliana Bana ISHII Amilcar ARENAL Yanise CHICOY Marcelo Beltrão MOLENTO Comparison of Two Methods for the Isolation of Genomic DNA from Cyathostomin Adult Parasites İstanbul Üniversitesi Veteriner Fakültesi Dergisi DNA extraction genomic material horse Strongylida; |
| title | Comparison of Two Methods for the Isolation of Genomic DNA from Cyathostomin Adult Parasites |
| title_full | Comparison of Two Methods for the Isolation of Genomic DNA from Cyathostomin Adult Parasites |
| title_fullStr | Comparison of Two Methods for the Isolation of Genomic DNA from Cyathostomin Adult Parasites |
| title_full_unstemmed | Comparison of Two Methods for the Isolation of Genomic DNA from Cyathostomin Adult Parasites |
| title_short | Comparison of Two Methods for the Isolation of Genomic DNA from Cyathostomin Adult Parasites |
| title_sort | comparison of two methods for the isolation of genomic dna from cyathostomin adult parasites |
| topic | DNA extraction genomic material horse Strongylida; |
| url | http://dergipark.gov.tr/iuvfd/issue/26805/320203 |
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