Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro
Platelet aggregometry assays are generally used for the analysis of platelet function but can also be adapted for further research and therapy focused applications. This method describes the procedures for the preparation of human platelet-rich plasma (PRP) and platelet-poor plasma (PPP) for the ass...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-01-01
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| Series: | MethodsX |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016118302152 |
| _version_ | 1811338327947739136 |
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| author | Alexandros Tsoupras Ioannis Zabetakis Ronan Lordan |
| author_facet | Alexandros Tsoupras Ioannis Zabetakis Ronan Lordan |
| author_sort | Alexandros Tsoupras |
| collection | DOAJ |
| description | Platelet aggregometry assays are generally used for the analysis of platelet function but can also be adapted for further research and therapy focused applications. This method describes the procedures for the preparation of human platelet-rich plasma (PRP) and platelet-poor plasma (PPP) for the assessment of human platelet aggregation induced by agonists such as platelet-activating factor (PAF), thrombin, collagen, adenosine diphosphate (ADP), arachidonic acid, etc. • This method can be applied in vitro to evaluate the aggregatory effects of these agonists and to assess the antiaggregatory effects of several bioactive antiplatelet agents (compounds of natural or pharmacological origin) in human PRP. • This versatile method can be used in both basic and clinical research for the assessment of platelet aggregation (a major cardiovascular risk factor), platelet agonists, and inhibitors, in physiological or pathological conditions. • This method can be adapted to assess platelet activity in postprandial and intervention studies ex vivo. Method name: Human platelet aggregometry assay, Keywords: Platelet aggregation, Light transmission aggregometry, Platelet-activating factor, Thrombin, Antiplatelet, Agonist, In vitro, Inhibitors |
| first_indexed | 2024-04-13T18:09:25Z |
| format | Article |
| id | doaj.art-86ff6a61c0574204b7825f2292a2c9cd |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-04-13T18:09:25Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-86ff6a61c0574204b7825f2292a2c9cd2022-12-22T02:35:58ZengElsevierMethodsX2215-01612019-01-0166370Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitroAlexandros Tsoupras0Ioannis Zabetakis1Ronan Lordan2Department of Biological Sciences, University of Limerick, Limerick, V94 T9PX, IrelandDepartment of Biological Sciences, University of Limerick, Limerick, V94 T9PX, IrelandCorresponding author.; Department of Biological Sciences, University of Limerick, Limerick, V94 T9PX, IrelandPlatelet aggregometry assays are generally used for the analysis of platelet function but can also be adapted for further research and therapy focused applications. This method describes the procedures for the preparation of human platelet-rich plasma (PRP) and platelet-poor plasma (PPP) for the assessment of human platelet aggregation induced by agonists such as platelet-activating factor (PAF), thrombin, collagen, adenosine diphosphate (ADP), arachidonic acid, etc. • This method can be applied in vitro to evaluate the aggregatory effects of these agonists and to assess the antiaggregatory effects of several bioactive antiplatelet agents (compounds of natural or pharmacological origin) in human PRP. • This versatile method can be used in both basic and clinical research for the assessment of platelet aggregation (a major cardiovascular risk factor), platelet agonists, and inhibitors, in physiological or pathological conditions. • This method can be adapted to assess platelet activity in postprandial and intervention studies ex vivo. Method name: Human platelet aggregometry assay, Keywords: Platelet aggregation, Light transmission aggregometry, Platelet-activating factor, Thrombin, Antiplatelet, Agonist, In vitro, Inhibitorshttp://www.sciencedirect.com/science/article/pii/S2215016118302152 |
| spellingShingle | Alexandros Tsoupras Ioannis Zabetakis Ronan Lordan Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro MethodsX |
| title | Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro |
| title_full | Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro |
| title_fullStr | Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro |
| title_full_unstemmed | Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro |
| title_short | Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro |
| title_sort | platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro |
| url | http://www.sciencedirect.com/science/article/pii/S2215016118302152 |
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