MeCP2 and transcriptional control of eukaryotic gene expression
Eukaryotic gene expression is controlled at multiple steps that work in harmony to ensure proper maintenance of cellular morphology and function. Such regulatory mechanisms would include transcriptional gene regulation, which is in turn controlled by chromatin remodeling, distinct topologically asso...
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Format: | Article |
Language: | English |
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Elsevier
2022-06-01
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Series: | European Journal of Cell Biology |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933522000401 |
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author | Ghanan Bin Akhtar Marjorie Buist Mojgan Rastegar |
author_facet | Ghanan Bin Akhtar Marjorie Buist Mojgan Rastegar |
author_sort | Ghanan Bin Akhtar |
collection | DOAJ |
description | Eukaryotic gene expression is controlled at multiple steps that work in harmony to ensure proper maintenance of cellular morphology and function. Such regulatory mechanisms would include transcriptional gene regulation, which is in turn controlled by chromatin remodeling, distinct topologically associating domains of the chromatin structure, cis-regulatory elements such as enhancers and promoters, action of trans-acting factors, DNA methylation, RNA modifications, and post-translational modification of histones. These guiding mechanisms of gene expression play critical roles in the epigenetic setting of individual cells within the eukaryotic systems. Some epigenetic factors may play multiple functional roles in guarding the accurate gene expression program of the eukaryotic cells, especially within the central nervous system. A well-studied example of such multi-functional factors is the methyl-CpG-binding protein 2 (MeCP2), a nuclear protein that is encoded by the X-linked MECP2 gene. Here, we aim to provide an overview of eukaryotic gene regulation, the three-dimensional chromatin organization, standard techniques to study newly synthesized RNA transcripts, and the role of MeCP2 as an important transcriptional regulator in eukaryotes. |
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institution | Directory Open Access Journal |
issn | 0171-9335 |
language | English |
last_indexed | 2024-04-13T11:37:39Z |
publishDate | 2022-06-01 |
publisher | Elsevier |
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series | European Journal of Cell Biology |
spelling | doaj.art-870191515f544271b8a8c4da2c5dd3632022-12-22T02:48:25ZengElsevierEuropean Journal of Cell Biology0171-93352022-06-011013151237MeCP2 and transcriptional control of eukaryotic gene expressionGhanan Bin Akhtar0Marjorie Buist1Mojgan Rastegar2Department of Biochemistry and Medical Genetics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, MB, CanadaDepartment of Biochemistry and Medical Genetics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, MB, CanadaCorrespondence to: Dr. Mojgan Rastegar, Department of Biochemistry and Medical Genetics, Max Rady College of Medicine, University of Manitoba, 745 Bannatyne Avenue, BMSB 627, Winnipeg, MB R3E 0J9, Canada.; Department of Biochemistry and Medical Genetics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, MB, CanadaEukaryotic gene expression is controlled at multiple steps that work in harmony to ensure proper maintenance of cellular morphology and function. Such regulatory mechanisms would include transcriptional gene regulation, which is in turn controlled by chromatin remodeling, distinct topologically associating domains of the chromatin structure, cis-regulatory elements such as enhancers and promoters, action of trans-acting factors, DNA methylation, RNA modifications, and post-translational modification of histones. These guiding mechanisms of gene expression play critical roles in the epigenetic setting of individual cells within the eukaryotic systems. Some epigenetic factors may play multiple functional roles in guarding the accurate gene expression program of the eukaryotic cells, especially within the central nervous system. A well-studied example of such multi-functional factors is the methyl-CpG-binding protein 2 (MeCP2), a nuclear protein that is encoded by the X-linked MECP2 gene. Here, we aim to provide an overview of eukaryotic gene regulation, the three-dimensional chromatin organization, standard techniques to study newly synthesized RNA transcripts, and the role of MeCP2 as an important transcriptional regulator in eukaryotes.http://www.sciencedirect.com/science/article/pii/S0171933522000401Transcriptional controlEpigeneticsDNA methylationGene regulationRNA polymeraseTopologically associating domains |
spellingShingle | Ghanan Bin Akhtar Marjorie Buist Mojgan Rastegar MeCP2 and transcriptional control of eukaryotic gene expression European Journal of Cell Biology Transcriptional control Epigenetics DNA methylation Gene regulation RNA polymerase Topologically associating domains |
title | MeCP2 and transcriptional control of eukaryotic gene expression |
title_full | MeCP2 and transcriptional control of eukaryotic gene expression |
title_fullStr | MeCP2 and transcriptional control of eukaryotic gene expression |
title_full_unstemmed | MeCP2 and transcriptional control of eukaryotic gene expression |
title_short | MeCP2 and transcriptional control of eukaryotic gene expression |
title_sort | mecp2 and transcriptional control of eukaryotic gene expression |
topic | Transcriptional control Epigenetics DNA methylation Gene regulation RNA polymerase Topologically associating domains |
url | http://www.sciencedirect.com/science/article/pii/S0171933522000401 |
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