Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA
Strangles is an economically important horse disease caused by Streptococcus equi subsp. equi. The diagnosis can be confirmed either directly by bacterial isolation and PCR or by ELISA, which is an indirect method based on the detection of serum antibodies. The aim of this study was to clone, expres...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
2014-08-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000401015&lng=en&tlng=en |
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| author | C.M. Moraes F.R. Conceição A.S.R. Rocha A.G. Santos Júnior L.M. Ribas A.P.C. Vargas C.E.W. Nogueira C. Gil-Turnes F.P.L. Leite |
| author_facet | C.M. Moraes F.R. Conceição A.S.R. Rocha A.G. Santos Júnior L.M. Ribas A.P.C. Vargas C.E.W. Nogueira C. Gil-Turnes F.P.L. Leite |
| author_sort | C.M. Moraes |
| collection | DOAJ |
| description | Strangles is an economically important horse disease caused by Streptococcus equi subsp. equi. The diagnosis can be confirmed either directly by bacterial isolation and PCR or by ELISA, which is an indirect method based on the detection of serum antibodies. The aim of this study was to clone, express and characterize the SeM protein of Streptococcus equi subsp. equi, evaluate its use as antigen in indirect ELISA and determine its performance to distinguish sera of negative, vaccinated and positive animals. This was initially performed by cloning the gene encoding the SeM protein and its expression in Escherichia coli. Subsequently, the protein produced was characterized and used as antigen in ELISA. Serum samples for evaluation were taken from 40 negative foals, 46 horses vaccinated with a commercial vaccine against strangles and 46 horses diagnosed with the disease. The test showed high specificity and sensitivity, allowing discrimination between negative and positive, positive and vaccinated animals, and vaccinated animals and negative sera. Thus, it was concluded that the protein produced rSeM, which can be used as antigen for disease diagnosis, and the described ELISA might be helpful to evaluate the immune status of the herd. |
| first_indexed | 2024-12-11T19:10:17Z |
| format | Article |
| id | doaj.art-87073aa76b5d4c5e8899ac8e62ed68e7 |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-11T19:10:17Z |
| publishDate | 2014-08-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-87073aa76b5d4c5e8899ac8e62ed68e72022-12-22T00:53:47ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622014-08-016641015102210.1590/1678-6034S0102-09352014000401015Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISAC.M. MoraesF.R. ConceiçãoA.S.R. RochaA.G. Santos JúniorL.M. RibasA.P.C. VargasC.E.W. NogueiraC. Gil-TurnesF.P.L. LeiteStrangles is an economically important horse disease caused by Streptococcus equi subsp. equi. The diagnosis can be confirmed either directly by bacterial isolation and PCR or by ELISA, which is an indirect method based on the detection of serum antibodies. The aim of this study was to clone, express and characterize the SeM protein of Streptococcus equi subsp. equi, evaluate its use as antigen in indirect ELISA and determine its performance to distinguish sera of negative, vaccinated and positive animals. This was initially performed by cloning the gene encoding the SeM protein and its expression in Escherichia coli. Subsequently, the protein produced was characterized and used as antigen in ELISA. Serum samples for evaluation were taken from 40 negative foals, 46 horses vaccinated with a commercial vaccine against strangles and 46 horses diagnosed with the disease. The test showed high specificity and sensitivity, allowing discrimination between negative and positive, positive and vaccinated animals, and vaccinated animals and negative sera. Thus, it was concluded that the protein produced rSeM, which can be used as antigen for disease diagnosis, and the described ELISA might be helpful to evaluate the immune status of the herd.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000401015&lng=en&tlng=enadenite equinaStreptococcus equiSeMELISA |
| spellingShingle | C.M. Moraes F.R. Conceição A.S.R. Rocha A.G. Santos Júnior L.M. Ribas A.P.C. Vargas C.E.W. Nogueira C. Gil-Turnes F.P.L. Leite Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA Arquivo Brasileiro de Medicina Veterinária e Zootecnia adenite equina Streptococcus equi SeM ELISA |
| title | Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA |
| title_full | Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA |
| title_fullStr | Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA |
| title_full_unstemmed | Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA |
| title_short | Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA |
| title_sort | cloning expression and characterization of sem protein of streptococcus equi subsp equi and evaluation of its use as antigen in an indirect elisa |
| topic | adenite equina Streptococcus equi SeM ELISA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000401015&lng=en&tlng=en |
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