Dynamics of Th9 cells and their potential role in immunopathogenesis of murine schistosomiasis
Abstract Background Th1, Th2, Th17, Treg and Tfh cells play important roles in schistosomiasis. Th9 cells secrete IL-9 as a signature cytokine and contribute to several classes of inflammatory disease. However, the effects of Th9 cells in schistosomiasis are unknown. We aimed to explore the dynamic...
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-017-2242-1 |
| _version_ | 1811282581295988736 |
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| author | Tingzheng Zhan Tingting Zhang Yanyan Wang Xiaoli Wang Cai Lin Huihui Ma Zhongliang Duan Chunxiang Li Jing Xu Chaoming Xia |
| author_facet | Tingzheng Zhan Tingting Zhang Yanyan Wang Xiaoli Wang Cai Lin Huihui Ma Zhongliang Duan Chunxiang Li Jing Xu Chaoming Xia |
| author_sort | Tingzheng Zhan |
| collection | DOAJ |
| description | Abstract Background Th1, Th2, Th17, Treg and Tfh cells play important roles in schistosomiasis. Th9 cells secrete IL-9 as a signature cytokine and contribute to several classes of inflammatory disease. However, the effects of Th9 cells in schistosomiasis are unknown. We aimed to explore the dynamic changes and potential roles of Th9 cells in the pathogenesis of hepatic egg granulomatous inflammation in mice infected with Schistosoma japonicum. Methods Twenty mice with S. japonicum infection and five normal controls (NC) were used as models. The average areas of egg granulomas were estimated by hematoxylin-eosin (H & E) staining. Hepatic IL-9 and transcription factor PU.1 levels were detected by immunohistochemistry. Flow cytometry techniques were used to analyze the proportions of Th9 cells. With the help of ELISA, serum levels of IL-9 were examined. Results The egg granulomas began to form from four weeks after infection and continued to develop. In parallel with the development of egg granulomas, the hepatic levels of IL-9 and PU.1 increased very slowly during the first four weeks post-infection and increased rapidly thereafter. Moreover, the proportions of splenic Th9 cells and levels of serum IL-9 had similar developmental trends with the egg granulomas. Conclusion The proliferation of Th9 cells and levels of IL-9 were significantly higher in S. japonicum-infected mice compared to NC. In addition, dynamic changes of Th9 and IL-9 were synchronous with the developmental trend of hepatic egg granulomatous inflammation, suggesting that Th9 cells might be a new subset in the pathogenesis of schistosomiasis. |
| first_indexed | 2024-04-13T01:55:08Z |
| format | Article |
| id | doaj.art-8707d720b85c46b6a675bb8a2e94a0a7 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-13T01:55:08Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-8707d720b85c46b6a675bb8a2e94a0a72022-12-22T03:07:47ZengBMCParasites & Vectors1756-33052017-06-011011810.1186/s13071-017-2242-1Dynamics of Th9 cells and their potential role in immunopathogenesis of murine schistosomiasisTingzheng Zhan0Tingting Zhang1Yanyan Wang2Xiaoli Wang3Cai Lin4Huihui Ma5Zhongliang Duan6Chunxiang Li7Jing Xu8Chaoming Xia9Department of Parasitology, Medical College of Soochow UniversityDepartment of Parasitology, Medical College of Soochow UniversityDepartment of Parasitology, Medical College of Soochow UniversityDepartment of Parasitology, Medical College of Soochow UniversityDepartment of Parasitology, Medical College of Soochow UniversityDepartment of Parasitology, Medical College of Soochow UniversityDepartment of Parasitology, Medical College of Soochow UniversityDepartment of Parasitology, Medical College of Soochow UniversityDepartment of Parasitology, Medical College of Soochow UniversityDepartment of Parasitology, Medical College of Soochow UniversityAbstract Background Th1, Th2, Th17, Treg and Tfh cells play important roles in schistosomiasis. Th9 cells secrete IL-9 as a signature cytokine and contribute to several classes of inflammatory disease. However, the effects of Th9 cells in schistosomiasis are unknown. We aimed to explore the dynamic changes and potential roles of Th9 cells in the pathogenesis of hepatic egg granulomatous inflammation in mice infected with Schistosoma japonicum. Methods Twenty mice with S. japonicum infection and five normal controls (NC) were used as models. The average areas of egg granulomas were estimated by hematoxylin-eosin (H & E) staining. Hepatic IL-9 and transcription factor PU.1 levels were detected by immunohistochemistry. Flow cytometry techniques were used to analyze the proportions of Th9 cells. With the help of ELISA, serum levels of IL-9 were examined. Results The egg granulomas began to form from four weeks after infection and continued to develop. In parallel with the development of egg granulomas, the hepatic levels of IL-9 and PU.1 increased very slowly during the first four weeks post-infection and increased rapidly thereafter. Moreover, the proportions of splenic Th9 cells and levels of serum IL-9 had similar developmental trends with the egg granulomas. Conclusion The proliferation of Th9 cells and levels of IL-9 were significantly higher in S. japonicum-infected mice compared to NC. In addition, dynamic changes of Th9 and IL-9 were synchronous with the developmental trend of hepatic egg granulomatous inflammation, suggesting that Th9 cells might be a new subset in the pathogenesis of schistosomiasis.http://link.springer.com/article/10.1186/s13071-017-2242-1Th9 cellInterleukin-9PU.1SchistosomiasisEgg granuloma |
| spellingShingle | Tingzheng Zhan Tingting Zhang Yanyan Wang Xiaoli Wang Cai Lin Huihui Ma Zhongliang Duan Chunxiang Li Jing Xu Chaoming Xia Dynamics of Th9 cells and their potential role in immunopathogenesis of murine schistosomiasis Parasites & Vectors Th9 cell Interleukin-9 PU.1 Schistosomiasis Egg granuloma |
| title | Dynamics of Th9 cells and their potential role in immunopathogenesis of murine schistosomiasis |
| title_full | Dynamics of Th9 cells and their potential role in immunopathogenesis of murine schistosomiasis |
| title_fullStr | Dynamics of Th9 cells and their potential role in immunopathogenesis of murine schistosomiasis |
| title_full_unstemmed | Dynamics of Th9 cells and their potential role in immunopathogenesis of murine schistosomiasis |
| title_short | Dynamics of Th9 cells and their potential role in immunopathogenesis of murine schistosomiasis |
| title_sort | dynamics of th9 cells and their potential role in immunopathogenesis of murine schistosomiasis |
| topic | Th9 cell Interleukin-9 PU.1 Schistosomiasis Egg granuloma |
| url | http://link.springer.com/article/10.1186/s13071-017-2242-1 |
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