Dynamics of germination stimulants dehydrocostus lactone and costunolide in the root exudates and extracts of sunflower
Orobanche cumana Wallr. (Orobanche cernua Loefl.) causes severe yield losses of confectionary sunflower in China. While germination of O. cumana is stimulated by sesquiterpene lactones (STLs) from host sunflower (Helianthus annuus L.). Dehydrocostus lactone and costunolide isolated from sunflower ro...
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
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| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2022.2025669 |
| _version_ | 1797351973538758656 |
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| author | Wenlong Wu Hongjuan Huang Jietian Su Xiaopeng Yun Yixiao Zhang Shouhui Wei Zhaofeng Huang Chaoxian Zhang Quanjiang Bai |
| author_facet | Wenlong Wu Hongjuan Huang Jietian Su Xiaopeng Yun Yixiao Zhang Shouhui Wei Zhaofeng Huang Chaoxian Zhang Quanjiang Bai |
| author_sort | Wenlong Wu |
| collection | DOAJ |
| description | Orobanche cumana Wallr. (Orobanche cernua Loefl.) causes severe yield losses of confectionary sunflower in China. While germination of O. cumana is stimulated by sesquiterpene lactones (STLs) from host sunflower (Helianthus annuus L.). Dehydrocostus lactone and costunolide isolated from sunflower root exudates are known as STLs to specifically induce O. cumana germination. Two major confectionary sunflower cultivars, SH363 (highly susceptible to O. cumana) and TH33 (resistant to O. cumana), were planted in China. However, STLs in these two sunflower cultivars has remained unknown. To identify STLs from root and exudates of sunflower for better understanding the role of stimulants in parasitic interaction of sunflower and O. cumana, we tested dehydrocostus lactone (DCL) and costunolide (CL) in root and root exudates of susceptible and resistant sunflower cultivars. The stimulant activity of sunflower root exudate and root extract to germination of O. cumana were also determined. Dehydrocostus lactone and costunolide were identified through ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS). Both DCL and CL were found in root extracts and root exudates in the whole tested time point from two sunflower cultivars. The concentration of dehydrocostus lactone was higher than that of costunolide at the same tested growth stage of each sunflower cultivar. It was observed that higher quantity of dehydrocostus lactone in susceptible cultivar than resistant cultivar of root and root exudates at later tested developmental stages. However, the amount of CL was no significant difference between SH363 and TH33 at all tested stages. The release amount of DCL from susceptible cultivar is 3.7 folds that of resistant cultivar at 28 DAT. These findings suggested that DCL was the one of the major signal compound in these two sunflower cultivars, and lower dehydrocostus lactone might contribute to the resistance of sunflower TH33 to O. cumana. |
| first_indexed | 2024-03-08T13:08:27Z |
| format | Article |
| id | doaj.art-870cfdaae443454896f590633a6dfb29 |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:27Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
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| series | Plant Signaling & Behavior |
| spelling | doaj.art-870cfdaae443454896f590633a6dfb292024-01-18T15:58:21ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242022-12-0117110.1080/15592324.2022.20256692025669Dynamics of germination stimulants dehydrocostus lactone and costunolide in the root exudates and extracts of sunflowerWenlong Wu0Hongjuan Huang1Jietian Su2Xiaopeng Yun3Yixiao Zhang4Shouhui Wei5Zhaofeng Huang6Chaoxian Zhang7Quanjiang Bai8Institute of Plant Protection Chinese Academy of Agricultural SciencesInstitute of Plant Protection Chinese Academy of Agricultural SciencesInstitute of Plant Protection Chinese Academy of Agricultural SciencesInstitute of Plant Protection, Inner Mongolia Academy of Agriculture and Animal Husbandry SciencesInstitute of Plant Protection Chinese Academy of Agricultural SciencesInstitute of Plant Protection Chinese Academy of Agricultural SciencesInstitute of Plant Protection Chinese Academy of Agricultural SciencesInstitute of Plant Protection Chinese Academy of Agricultural SciencesInstitute of Plant Protection, Inner Mongolia Academy of Agriculture and Animal Husbandry SciencesOrobanche cumana Wallr. (Orobanche cernua Loefl.) causes severe yield losses of confectionary sunflower in China. While germination of O. cumana is stimulated by sesquiterpene lactones (STLs) from host sunflower (Helianthus annuus L.). Dehydrocostus lactone and costunolide isolated from sunflower root exudates are known as STLs to specifically induce O. cumana germination. Two major confectionary sunflower cultivars, SH363 (highly susceptible to O. cumana) and TH33 (resistant to O. cumana), were planted in China. However, STLs in these two sunflower cultivars has remained unknown. To identify STLs from root and exudates of sunflower for better understanding the role of stimulants in parasitic interaction of sunflower and O. cumana, we tested dehydrocostus lactone (DCL) and costunolide (CL) in root and root exudates of susceptible and resistant sunflower cultivars. The stimulant activity of sunflower root exudate and root extract to germination of O. cumana were also determined. Dehydrocostus lactone and costunolide were identified through ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS). Both DCL and CL were found in root extracts and root exudates in the whole tested time point from two sunflower cultivars. The concentration of dehydrocostus lactone was higher than that of costunolide at the same tested growth stage of each sunflower cultivar. It was observed that higher quantity of dehydrocostus lactone in susceptible cultivar than resistant cultivar of root and root exudates at later tested developmental stages. However, the amount of CL was no significant difference between SH363 and TH33 at all tested stages. The release amount of DCL from susceptible cultivar is 3.7 folds that of resistant cultivar at 28 DAT. These findings suggested that DCL was the one of the major signal compound in these two sunflower cultivars, and lower dehydrocostus lactone might contribute to the resistance of sunflower TH33 to O. cumana.http://dx.doi.org/10.1080/15592324.2022.2025669sunflowerorobanche cumana loefldehydrocostus lactonecostunolide |
| spellingShingle | Wenlong Wu Hongjuan Huang Jietian Su Xiaopeng Yun Yixiao Zhang Shouhui Wei Zhaofeng Huang Chaoxian Zhang Quanjiang Bai Dynamics of germination stimulants dehydrocostus lactone and costunolide in the root exudates and extracts of sunflower Plant Signaling & Behavior sunflower orobanche cumana loefl dehydrocostus lactone costunolide |
| title | Dynamics of germination stimulants dehydrocostus lactone and costunolide in the root exudates and extracts of sunflower |
| title_full | Dynamics of germination stimulants dehydrocostus lactone and costunolide in the root exudates and extracts of sunflower |
| title_fullStr | Dynamics of germination stimulants dehydrocostus lactone and costunolide in the root exudates and extracts of sunflower |
| title_full_unstemmed | Dynamics of germination stimulants dehydrocostus lactone and costunolide in the root exudates and extracts of sunflower |
| title_short | Dynamics of germination stimulants dehydrocostus lactone and costunolide in the root exudates and extracts of sunflower |
| title_sort | dynamics of germination stimulants dehydrocostus lactone and costunolide in the root exudates and extracts of sunflower |
| topic | sunflower orobanche cumana loefl dehydrocostus lactone costunolide |
| url | http://dx.doi.org/10.1080/15592324.2022.2025669 |
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