Functionalization with a Polyphenol-Rich Pomace Extract Empowers a Ceramic Bone Filler with In Vitro Antioxidant, Anti-Inflammatory, and Pro-Osteogenic Properties
Oral diseases and periodontitis in particular are a major health burden worldwide, because of their association with various systemic diseases and with conditions such as peri-implantitis. Attempts have been made over the years to reverse bone loss due to the host disproportionate inflammatory respo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/12/2/31 |
| _version_ | 1797535157575483392 |
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| author | Giorgio Iviglia Elisa Torre Clara Cassinelli Marco Morra |
| author_facet | Giorgio Iviglia Elisa Torre Clara Cassinelli Marco Morra |
| author_sort | Giorgio Iviglia |
| collection | DOAJ |
| description | Oral diseases and periodontitis in particular are a major health burden worldwide, because of their association with various systemic diseases and with conditions such as peri-implantitis. Attempts have been made over the years to reverse bone loss due to the host disproportionate inflammatory response and to prevent failure of dental implants. To this end, the use of biomaterials functionalized with molecules characterized by anti-inflammatory and antioxidant properties could represent a new frontier for regenerating functional periodontal tissues. In this study, a new ceramic granulated biomaterial, named Synergoss Red (SR), functionalized with a polyphenolic mixture extracted from pomace of the Croatina grape variety, is introduced. Following a preliminary in-depth characterization of the extract by HPLC analysis and of the biomaterial surface and composition, we performed evaluations of cytocompatibility and a biological response through in vitro assays. The anti-inflammatory and antioxidant properties of the identified phenolic molecules contained in SR were shown to downregulate inflammation in macrophages, to stimulate in osteoblast-like cells the expression of genes involved in deposition of the early bone matrix, and to mitigate bone remodeling by decreasing the RANKL/OPG ratio. Thanks to its cytocompatibility and assorted beneficial effects on bone regeneration, SR could be considered an innovative regenerative approach in periodontal therapy. |
| first_indexed | 2024-03-10T11:41:31Z |
| format | Article |
| id | doaj.art-870d73e687b647df9abfa8e24b69f347 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T11:41:31Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-870d73e687b647df9abfa8e24b69f3472023-11-21T18:28:29ZengMDPI AGJournal of Functional Biomaterials2079-49832021-05-011223110.3390/jfb12020031Functionalization with a Polyphenol-Rich Pomace Extract Empowers a Ceramic Bone Filler with In Vitro Antioxidant, Anti-Inflammatory, and Pro-Osteogenic PropertiesGiorgio Iviglia0Elisa Torre1Clara Cassinelli2Marco Morra3Nobil Bio Ricerche srl, Via Valcastellana 26, 14037 Portacomaro, AT, ItalyNobil Bio Ricerche srl, Via Valcastellana 26, 14037 Portacomaro, AT, ItalyNobil Bio Ricerche srl, Via Valcastellana 26, 14037 Portacomaro, AT, ItalyNobil Bio Ricerche srl, Via Valcastellana 26, 14037 Portacomaro, AT, ItalyOral diseases and periodontitis in particular are a major health burden worldwide, because of their association with various systemic diseases and with conditions such as peri-implantitis. Attempts have been made over the years to reverse bone loss due to the host disproportionate inflammatory response and to prevent failure of dental implants. To this end, the use of biomaterials functionalized with molecules characterized by anti-inflammatory and antioxidant properties could represent a new frontier for regenerating functional periodontal tissues. In this study, a new ceramic granulated biomaterial, named Synergoss Red (SR), functionalized with a polyphenolic mixture extracted from pomace of the Croatina grape variety, is introduced. Following a preliminary in-depth characterization of the extract by HPLC analysis and of the biomaterial surface and composition, we performed evaluations of cytocompatibility and a biological response through in vitro assays. The anti-inflammatory and antioxidant properties of the identified phenolic molecules contained in SR were shown to downregulate inflammation in macrophages, to stimulate in osteoblast-like cells the expression of genes involved in deposition of the early bone matrix, and to mitigate bone remodeling by decreasing the RANKL/OPG ratio. Thanks to its cytocompatibility and assorted beneficial effects on bone regeneration, SR could be considered an innovative regenerative approach in periodontal therapy.https://www.mdpi.com/2079-4983/12/2/31bone fillerperi-implantitispolyphenolsliquid chromatographyantioxidantanti-inflammatory |
| spellingShingle | Giorgio Iviglia Elisa Torre Clara Cassinelli Marco Morra Functionalization with a Polyphenol-Rich Pomace Extract Empowers a Ceramic Bone Filler with In Vitro Antioxidant, Anti-Inflammatory, and Pro-Osteogenic Properties Journal of Functional Biomaterials bone filler peri-implantitis polyphenols liquid chromatography antioxidant anti-inflammatory |
| title | Functionalization with a Polyphenol-Rich Pomace Extract Empowers a Ceramic Bone Filler with In Vitro Antioxidant, Anti-Inflammatory, and Pro-Osteogenic Properties |
| title_full | Functionalization with a Polyphenol-Rich Pomace Extract Empowers a Ceramic Bone Filler with In Vitro Antioxidant, Anti-Inflammatory, and Pro-Osteogenic Properties |
| title_fullStr | Functionalization with a Polyphenol-Rich Pomace Extract Empowers a Ceramic Bone Filler with In Vitro Antioxidant, Anti-Inflammatory, and Pro-Osteogenic Properties |
| title_full_unstemmed | Functionalization with a Polyphenol-Rich Pomace Extract Empowers a Ceramic Bone Filler with In Vitro Antioxidant, Anti-Inflammatory, and Pro-Osteogenic Properties |
| title_short | Functionalization with a Polyphenol-Rich Pomace Extract Empowers a Ceramic Bone Filler with In Vitro Antioxidant, Anti-Inflammatory, and Pro-Osteogenic Properties |
| title_sort | functionalization with a polyphenol rich pomace extract empowers a ceramic bone filler with in vitro antioxidant anti inflammatory and pro osteogenic properties |
| topic | bone filler peri-implantitis polyphenols liquid chromatography antioxidant anti-inflammatory |
| url | https://www.mdpi.com/2079-4983/12/2/31 |
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