Aerobic exercise alters DNA hydroxymethylation levels in an experimental rodent model of temporal lobe epilepsy
The therapeutic potential of aerobic exercise in mitigating seizures and cognitive issues in temporal lobe epilepsy (TLE) is recognized, yet the underlying mechanisms are not well understood. Using a rodent TLE model induced by Kainic acid (KA), we investigated the impact of a single bout of exercis...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Epilepsy & Behavior Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589986423000606 |
| _version_ | 1797281198891859968 |
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| author | Silvienne C. Sint Jago Rudhab Bahabry Anna Maria Schreiber Julia Homola Tram Ngyuen Fernando Meijia Jane B. Allendorfer Farah D. Lubin |
| author_facet | Silvienne C. Sint Jago Rudhab Bahabry Anna Maria Schreiber Julia Homola Tram Ngyuen Fernando Meijia Jane B. Allendorfer Farah D. Lubin |
| author_sort | Silvienne C. Sint Jago |
| collection | DOAJ |
| description | The therapeutic potential of aerobic exercise in mitigating seizures and cognitive issues in temporal lobe epilepsy (TLE) is recognized, yet the underlying mechanisms are not well understood. Using a rodent TLE model induced by Kainic acid (KA), we investigated the impact of a single bout of exercise (i.e., acute) or 4 weeks of aerobic exercise (i.e., chronic). Blood was processed for epilepsy-associated serum markers, and DNA methylation (DNAme), and hippocampal area CA3 was assessed for gene expression levels for DNAme-associated enzymes. While acute aerobic exercise did not alter serum Brain-Derived Neurotrophic Factor (BDNF) or Interleukin-6 (IL-6), chronic exercise resulted in an exercise-specific decrease in serum BDNF and an increase in serum IL-6 levels in epileptic rats. Additionally, whole blood DNAme levels, specifically 5-hydroxymethylcytosine (5-hmC), decreased in epileptic animals following chronic exercise. Hippocampal CA3 5-hmC levels and ten-eleven translocation protein (TET1) expression mirrored these changes. Furthermore, immunohistochemistry analysis revealed that most 5-hmC changes in response to chronic exercise were neuron-specific within area CA3 of the hippocampus. Together, these findings suggest that DNAme mechanisms in the rodent model of TLE are responsive to chronic aerobic exercise, with emphasis on neuronal 5-hmC DNAme in the epileptic hippocampus. |
| first_indexed | 2024-03-07T16:53:09Z |
| format | Article |
| id | doaj.art-870e488345bc4496980870f0197d40e4 |
| institution | Directory Open Access Journal |
| issn | 2589-9864 |
| language | English |
| last_indexed | 2024-03-07T16:53:09Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Epilepsy & Behavior Reports |
| spelling | doaj.art-870e488345bc4496980870f0197d40e42024-03-03T04:30:05ZengElsevierEpilepsy & Behavior Reports2589-98642024-01-0125100642Aerobic exercise alters DNA hydroxymethylation levels in an experimental rodent model of temporal lobe epilepsySilvienne C. Sint Jago0Rudhab Bahabry1Anna Maria Schreiber2Julia Homola3Tram Ngyuen4Fernando Meijia5Jane B. Allendorfer6Farah D. Lubin7Department of Neurobiology, University of Alabama at Birmingham, United StatesDepartment of Neurobiology, University of Alabama at Birmingham, United StatesDepartment of Neurobiology, University of Alabama at Birmingham, United StatesDepartment of Neurobiology, University of Alabama at Birmingham, United StatesDepartment of Neurobiology, University of Alabama at Birmingham, United StatesDepartment of Neurobiology, University of Alabama at Birmingham, United StatesDepartment of Neurobiology, University of Alabama at Birmingham, United States; Department of Neurology, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Neurobiology, University of Alabama at Birmingham, United States; Corresponding author at: Department of Neurobiology, Shelby Building, University of Alabama at Birmingham, 1825 University Boulevard, Birmingham, AL 35294, United States.The therapeutic potential of aerobic exercise in mitigating seizures and cognitive issues in temporal lobe epilepsy (TLE) is recognized, yet the underlying mechanisms are not well understood. Using a rodent TLE model induced by Kainic acid (KA), we investigated the impact of a single bout of exercise (i.e., acute) or 4 weeks of aerobic exercise (i.e., chronic). Blood was processed for epilepsy-associated serum markers, and DNA methylation (DNAme), and hippocampal area CA3 was assessed for gene expression levels for DNAme-associated enzymes. While acute aerobic exercise did not alter serum Brain-Derived Neurotrophic Factor (BDNF) or Interleukin-6 (IL-6), chronic exercise resulted in an exercise-specific decrease in serum BDNF and an increase in serum IL-6 levels in epileptic rats. Additionally, whole blood DNAme levels, specifically 5-hydroxymethylcytosine (5-hmC), decreased in epileptic animals following chronic exercise. Hippocampal CA3 5-hmC levels and ten-eleven translocation protein (TET1) expression mirrored these changes. Furthermore, immunohistochemistry analysis revealed that most 5-hmC changes in response to chronic exercise were neuron-specific within area CA3 of the hippocampus. Together, these findings suggest that DNAme mechanisms in the rodent model of TLE are responsive to chronic aerobic exercise, with emphasis on neuronal 5-hmC DNAme in the epileptic hippocampus.http://www.sciencedirect.com/science/article/pii/S2589986423000606EpigeneticsNeuronsAstrocytes5-hydroxymethylcytosineTen-eleven translocationChronic exercise |
| spellingShingle | Silvienne C. Sint Jago Rudhab Bahabry Anna Maria Schreiber Julia Homola Tram Ngyuen Fernando Meijia Jane B. Allendorfer Farah D. Lubin Aerobic exercise alters DNA hydroxymethylation levels in an experimental rodent model of temporal lobe epilepsy Epilepsy & Behavior Reports Epigenetics Neurons Astrocytes 5-hydroxymethylcytosine Ten-eleven translocation Chronic exercise |
| title | Aerobic exercise alters DNA hydroxymethylation levels in an experimental rodent model of temporal lobe epilepsy |
| title_full | Aerobic exercise alters DNA hydroxymethylation levels in an experimental rodent model of temporal lobe epilepsy |
| title_fullStr | Aerobic exercise alters DNA hydroxymethylation levels in an experimental rodent model of temporal lobe epilepsy |
| title_full_unstemmed | Aerobic exercise alters DNA hydroxymethylation levels in an experimental rodent model of temporal lobe epilepsy |
| title_short | Aerobic exercise alters DNA hydroxymethylation levels in an experimental rodent model of temporal lobe epilepsy |
| title_sort | aerobic exercise alters dna hydroxymethylation levels in an experimental rodent model of temporal lobe epilepsy |
| topic | Epigenetics Neurons Astrocytes 5-hydroxymethylcytosine Ten-eleven translocation Chronic exercise |
| url | http://www.sciencedirect.com/science/article/pii/S2589986423000606 |
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