Pollen Application Methods Affecting Fruit Quality and Seed Formation in Artificial Pollination of Yellow-Fleshed Kiwifruit
This study investigated pollen application methods for artificial pollination in tetraploid kiwifruit cultivars ‘Halla Gold’ and ‘Sweet Gold’ grown in a nonheated plastic-film house in Jeju, Korea. Pollen of the hexaploid cultivar ‘Bohwa’ (<i>A. chinensis</i> var. <i>deliciosa</...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/2/150 |
| _version_ | 1797479599767027712 |
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| author | Eun Ui Oh Seong Cheol Kim Mock Hee Lee Kwan Jeong Song |
| author_facet | Eun Ui Oh Seong Cheol Kim Mock Hee Lee Kwan Jeong Song |
| author_sort | Eun Ui Oh |
| collection | DOAJ |
| description | This study investigated pollen application methods for artificial pollination in tetraploid kiwifruit cultivars ‘Halla Gold’ and ‘Sweet Gold’ grown in a nonheated plastic-film house in Jeju, Korea. Pollen of the hexaploid cultivar ‘Bohwa’ (<i>A. chinensis</i> var. <i>deliciosa</i>) bred in Korea was used for artificial pollination. We examined the effect of repeated pollination, pretreatment of stigma with wetting materials, application of dry pollen or pollen in suspension on fruit quality, and seed formation. With repeated pollination, pollen tubes in the pistil reached and penetrated the ovule three days after artificial pollination, although the pattern varied depending on the number of dry pollen applications. In both cultivars, the number of pollen tubes was clearly higher following repeated pollination than following single pollination, and fruit weight, dry matter (DM), number of seeds, and 100-seed weight were also higher. When pistillate flowers were pollinated with dry pollen immediately after water sprinkle, both cultivars showed the lowest fruit weight, DM, firmness, number of seeds, and 100-seed weight, whereas there were no significant differences in fruit quality or seed formation for dry pollen application 1 h after water sprinkle, or immediately or 1 h after suspension medium sprinkle. For pollination using a pollen suspension, the fruit weight was lower in both cultivars. There were no significant differences in fruit quality and seed formation following application of dry pollen or a pollen suspension, except for fruit weight in ‘Sweet Gold’. It could be seen from the results of this study that raindrops or dewdrops on the stigma might reduce the efficiency of artificial pollination using dry pollen. Still, the application of repeated pollination enhanced the efficiency of artificial pollination. |
| first_indexed | 2024-03-09T21:48:05Z |
| format | Article |
| id | doaj.art-870e838c2e6b4016ac402a47eb718599 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T21:48:05Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-870e838c2e6b4016ac402a47eb7185992023-11-23T20:12:27ZengMDPI AGHorticulturae2311-75242022-02-018215010.3390/horticulturae8020150Pollen Application Methods Affecting Fruit Quality and Seed Formation in Artificial Pollination of Yellow-Fleshed KiwifruitEun Ui Oh0Seong Cheol Kim1Mock Hee Lee2Kwan Jeong Song3Department of Horticultural Science, Jeju National Universtiy, Jeju 63243, KoreaResearch Institute of Climate Change and Agriculture, National Institute of Horticultural & Herbal Science, Rural Development Administration, Jeju 63240, KoreaNamhae Branch, National Institute of Horticultural & Herbal Science, Rural Development Administration, Namhae 52430, KoreaDepartment of Horticultural Science, Jeju National Universtiy, Jeju 63243, KoreaThis study investigated pollen application methods for artificial pollination in tetraploid kiwifruit cultivars ‘Halla Gold’ and ‘Sweet Gold’ grown in a nonheated plastic-film house in Jeju, Korea. Pollen of the hexaploid cultivar ‘Bohwa’ (<i>A. chinensis</i> var. <i>deliciosa</i>) bred in Korea was used for artificial pollination. We examined the effect of repeated pollination, pretreatment of stigma with wetting materials, application of dry pollen or pollen in suspension on fruit quality, and seed formation. With repeated pollination, pollen tubes in the pistil reached and penetrated the ovule three days after artificial pollination, although the pattern varied depending on the number of dry pollen applications. In both cultivars, the number of pollen tubes was clearly higher following repeated pollination than following single pollination, and fruit weight, dry matter (DM), number of seeds, and 100-seed weight were also higher. When pistillate flowers were pollinated with dry pollen immediately after water sprinkle, both cultivars showed the lowest fruit weight, DM, firmness, number of seeds, and 100-seed weight, whereas there were no significant differences in fruit quality or seed formation for dry pollen application 1 h after water sprinkle, or immediately or 1 h after suspension medium sprinkle. For pollination using a pollen suspension, the fruit weight was lower in both cultivars. There were no significant differences in fruit quality and seed formation following application of dry pollen or a pollen suspension, except for fruit weight in ‘Sweet Gold’. It could be seen from the results of this study that raindrops or dewdrops on the stigma might reduce the efficiency of artificial pollination using dry pollen. Still, the application of repeated pollination enhanced the efficiency of artificial pollination.https://www.mdpi.com/2311-7524/8/2/150dry pollenpollen suspensionpollen tube growthrepeated pollinationstigma desiccation |
| spellingShingle | Eun Ui Oh Seong Cheol Kim Mock Hee Lee Kwan Jeong Song Pollen Application Methods Affecting Fruit Quality and Seed Formation in Artificial Pollination of Yellow-Fleshed Kiwifruit Horticulturae dry pollen pollen suspension pollen tube growth repeated pollination stigma desiccation |
| title | Pollen Application Methods Affecting Fruit Quality and Seed Formation in Artificial Pollination of Yellow-Fleshed Kiwifruit |
| title_full | Pollen Application Methods Affecting Fruit Quality and Seed Formation in Artificial Pollination of Yellow-Fleshed Kiwifruit |
| title_fullStr | Pollen Application Methods Affecting Fruit Quality and Seed Formation in Artificial Pollination of Yellow-Fleshed Kiwifruit |
| title_full_unstemmed | Pollen Application Methods Affecting Fruit Quality and Seed Formation in Artificial Pollination of Yellow-Fleshed Kiwifruit |
| title_short | Pollen Application Methods Affecting Fruit Quality and Seed Formation in Artificial Pollination of Yellow-Fleshed Kiwifruit |
| title_sort | pollen application methods affecting fruit quality and seed formation in artificial pollination of yellow fleshed kiwifruit |
| topic | dry pollen pollen suspension pollen tube growth repeated pollination stigma desiccation |
| url | https://www.mdpi.com/2311-7524/8/2/150 |
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