Overexpression of the secretory small GTPase Rab27B in human breast cancer correlates closely with lymph node metastasis and predicts poor prognosis
<p>Abstract</p> <p>Background</p> <p>The secretory small GTPase Rab27b was recently identified as an oncogene in breast cancer (BC) in vivo and in vitro studies. This research was designed to further explore the clinical and prognostic significance of Rab27B in BC patie...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-12-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://www.translational-medicine.com/content/10/1/242 |
| _version_ | 1828543540631699456 |
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| author | Zhang Jia-Xing Huang Xiao-Xia Cai Man-Bo Tong Zhu-Ting Chen Jie-Wei Qian Dong Liao Yi-Ji Deng Hai-Xia Liao Ding-Zhun Huang Ma-Yan Zeng Yi-Xin Xie Dan Mai Shi-Juan |
| author_facet | Zhang Jia-Xing Huang Xiao-Xia Cai Man-Bo Tong Zhu-Ting Chen Jie-Wei Qian Dong Liao Yi-Ji Deng Hai-Xia Liao Ding-Zhun Huang Ma-Yan Zeng Yi-Xin Xie Dan Mai Shi-Juan |
| author_sort | Zhang Jia-Xing |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The secretory small GTPase Rab27b was recently identified as an oncogene in breast cancer (BC) in vivo and in vitro studies. This research was designed to further explore the clinical and prognostic significance of Rab27B in BC patients.</p> <p>Methods</p> <p>The mRNA/protein expression level of Rab27B was examined by performing Real-time PCR, western blot, and immunohistochemistry (IHC) assays in 12 paired BC tissues and matched adjacent noncancerous tissues (NAT). Then we carried out IHC assay in a large cohort of 221 invasive BC tissues, 22 normal breast tissues, 40 fibroadenoma (FA), 30 ductual carcinoma in situ (DCIS) and 40 metastatic lymph nodes (LNs). The receiver operating characteristic curve method was applied to obtain the optimal cutoff value for high Rab27B expression. Epithelial-mesenchymal transition (EMT) marker expression levels were detected in relation to Rab27B expression.</p> <p>Results</p> <p>We observed that the increased expression of Rab27B was dependent upon the magnitude of cancer progression (<it>P</it> < 0.001). The elevated expression of Rab27B was closely correlated with lymph node metastasis, advanced clinical stage, ascending pathology classification, and positive ER status. Furthermore, patients with high expression of Rab27B had inferior survival outcomes. Multivariate Cox regression analysis proved that Rab27B was a significantly independent risk factor for patients’ survival (<it>P</it> < 0.001). Furthermore, a significant positive relationship was observed between Rab27B expression and elevated mesenchymal EMT markers.</p> <p>Conclusion</p> <p>Our findings suggest that overexpression of Rab27B in BC coincides with lymph node metastasis and acquisition of a poor prognostic phenotype.</p> |
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| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-12T02:16:03Z |
| publishDate | 2012-12-01 |
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| spelling | doaj.art-871d7c0a15f5412cb3965fff043574b12022-12-22T00:41:48ZengBMCJournal of Translational Medicine1479-58762012-12-0110124210.1186/1479-5876-10-242Overexpression of the secretory small GTPase Rab27B in human breast cancer correlates closely with lymph node metastasis and predicts poor prognosisZhang Jia-XingHuang Xiao-XiaCai Man-BoTong Zhu-TingChen Jie-WeiQian DongLiao Yi-JiDeng Hai-XiaLiao Ding-ZhunHuang Ma-YanZeng Yi-XinXie DanMai Shi-Juan<p>Abstract</p> <p>Background</p> <p>The secretory small GTPase Rab27b was recently identified as an oncogene in breast cancer (BC) in vivo and in vitro studies. This research was designed to further explore the clinical and prognostic significance of Rab27B in BC patients.</p> <p>Methods</p> <p>The mRNA/protein expression level of Rab27B was examined by performing Real-time PCR, western blot, and immunohistochemistry (IHC) assays in 12 paired BC tissues and matched adjacent noncancerous tissues (NAT). Then we carried out IHC assay in a large cohort of 221 invasive BC tissues, 22 normal breast tissues, 40 fibroadenoma (FA), 30 ductual carcinoma in situ (DCIS) and 40 metastatic lymph nodes (LNs). The receiver operating characteristic curve method was applied to obtain the optimal cutoff value for high Rab27B expression. Epithelial-mesenchymal transition (EMT) marker expression levels were detected in relation to Rab27B expression.</p> <p>Results</p> <p>We observed that the increased expression of Rab27B was dependent upon the magnitude of cancer progression (<it>P</it> < 0.001). The elevated expression of Rab27B was closely correlated with lymph node metastasis, advanced clinical stage, ascending pathology classification, and positive ER status. Furthermore, patients with high expression of Rab27B had inferior survival outcomes. Multivariate Cox regression analysis proved that Rab27B was a significantly independent risk factor for patients’ survival (<it>P</it> < 0.001). Furthermore, a significant positive relationship was observed between Rab27B expression and elevated mesenchymal EMT markers.</p> <p>Conclusion</p> <p>Our findings suggest that overexpression of Rab27B in BC coincides with lymph node metastasis and acquisition of a poor prognostic phenotype.</p>http://www.translational-medicine.com/content/10/1/242Rab27BBreast cancerPrognosisEMTMetastasis |
| spellingShingle | Zhang Jia-Xing Huang Xiao-Xia Cai Man-Bo Tong Zhu-Ting Chen Jie-Wei Qian Dong Liao Yi-Ji Deng Hai-Xia Liao Ding-Zhun Huang Ma-Yan Zeng Yi-Xin Xie Dan Mai Shi-Juan Overexpression of the secretory small GTPase Rab27B in human breast cancer correlates closely with lymph node metastasis and predicts poor prognosis Journal of Translational Medicine Rab27B Breast cancer Prognosis EMT Metastasis |
| title | Overexpression of the secretory small GTPase Rab27B in human breast cancer correlates closely with lymph node metastasis and predicts poor prognosis |
| title_full | Overexpression of the secretory small GTPase Rab27B in human breast cancer correlates closely with lymph node metastasis and predicts poor prognosis |
| title_fullStr | Overexpression of the secretory small GTPase Rab27B in human breast cancer correlates closely with lymph node metastasis and predicts poor prognosis |
| title_full_unstemmed | Overexpression of the secretory small GTPase Rab27B in human breast cancer correlates closely with lymph node metastasis and predicts poor prognosis |
| title_short | Overexpression of the secretory small GTPase Rab27B in human breast cancer correlates closely with lymph node metastasis and predicts poor prognosis |
| title_sort | overexpression of the secretory small gtpase rab27b in human breast cancer correlates closely with lymph node metastasis and predicts poor prognosis |
| topic | Rab27B Breast cancer Prognosis EMT Metastasis |
| url | http://www.translational-medicine.com/content/10/1/242 |
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