454 Genome Sequencing of Pseudoperonospora cubensis Reveals Effector Proteins with a QXLR Translocation Motif
Pseudoperonospora cubensis is a biotrophic oomycete pathogen that causes downy mildew of cucurbits, a devastating foliar disease threatening cucurbit production worldwide. We sequenced P. cubensis genomic DNA using 454 pyrosequencing and obtained random genomic sequences covering approximately 14% o...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2011-05-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-10-0185 |
| _version_ | 1818647233732542464 |
|---|---|
| author | Miaoying Tian Joe Win Elizabeth Savory Alyssa Burkhardt Michael Held Federica Brandizzi Brad Day |
| author_facet | Miaoying Tian Joe Win Elizabeth Savory Alyssa Burkhardt Michael Held Federica Brandizzi Brad Day |
| author_sort | Miaoying Tian |
| collection | DOAJ |
| description | Pseudoperonospora cubensis is a biotrophic oomycete pathogen that causes downy mildew of cucurbits, a devastating foliar disease threatening cucurbit production worldwide. We sequenced P. cubensis genomic DNA using 454 pyrosequencing and obtained random genomic sequences covering approximately 14% of the genome, thus providing the first set of useful genomic sequence information for P. cubensis. Using bioinformatics approaches, we identified 32 putative RXLR effector proteins. Interestingly, we also identified 29 secreted peptides with high similarity to RXLR effectors at the N-terminal translocation domain, yet containing an R-to-Q substitution in the first residue of the translocation motif. Among these, a family of QXLR-containing proteins, designated as PcQNE, was confirmed to have a functional signal peptide and was further characterized as being localized in the plant nucleus. Internalization of secreted PcQNE into plant cells requires the QXLR-EER motif. This family has a large number of near-identical copies within the P. cubensis genome, is under diversifying selection at the C-terminal domain, and is upregulated during infection of plants, all of which are common characteristics of characterized oomycete effectors. Taken together, the data suggest that PcQNE are bona fide effector proteins with a QXLR translocation motif, and QXLR effectors are prevalent in P. cubensis. Furthermore, the massive duplication of PcQNE suggests that they might play pivotal roles in pathogen fitness and pathogenicity. |
| first_indexed | 2024-12-17T00:59:17Z |
| format | Article |
| id | doaj.art-871fb4b5278b4205a141998144e4096b |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T00:59:17Z |
| publishDate | 2011-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-871fb4b5278b4205a141998144e4096b2022-12-21T22:09:32ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062011-05-0124554355310.1094/MPMI-08-10-0185454 Genome Sequencing of Pseudoperonospora cubensis Reveals Effector Proteins with a QXLR Translocation MotifMiaoying TianJoe WinElizabeth SavoryAlyssa BurkhardtMichael HeldFederica BrandizziBrad DayPseudoperonospora cubensis is a biotrophic oomycete pathogen that causes downy mildew of cucurbits, a devastating foliar disease threatening cucurbit production worldwide. We sequenced P. cubensis genomic DNA using 454 pyrosequencing and obtained random genomic sequences covering approximately 14% of the genome, thus providing the first set of useful genomic sequence information for P. cubensis. Using bioinformatics approaches, we identified 32 putative RXLR effector proteins. Interestingly, we also identified 29 secreted peptides with high similarity to RXLR effectors at the N-terminal translocation domain, yet containing an R-to-Q substitution in the first residue of the translocation motif. Among these, a family of QXLR-containing proteins, designated as PcQNE, was confirmed to have a functional signal peptide and was further characterized as being localized in the plant nucleus. Internalization of secreted PcQNE into plant cells requires the QXLR-EER motif. This family has a large number of near-identical copies within the P. cubensis genome, is under diversifying selection at the C-terminal domain, and is upregulated during infection of plants, all of which are common characteristics of characterized oomycete effectors. Taken together, the data suggest that PcQNE are bona fide effector proteins with a QXLR translocation motif, and QXLR effectors are prevalent in P. cubensis. Furthermore, the massive duplication of PcQNE suggests that they might play pivotal roles in pathogen fitness and pathogenicity.https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-10-0185 |
| spellingShingle | Miaoying Tian Joe Win Elizabeth Savory Alyssa Burkhardt Michael Held Federica Brandizzi Brad Day 454 Genome Sequencing of Pseudoperonospora cubensis Reveals Effector Proteins with a QXLR Translocation Motif Molecular Plant-Microbe Interactions |
| title | 454 Genome Sequencing of Pseudoperonospora cubensis Reveals Effector Proteins with a QXLR Translocation Motif |
| title_full | 454 Genome Sequencing of Pseudoperonospora cubensis Reveals Effector Proteins with a QXLR Translocation Motif |
| title_fullStr | 454 Genome Sequencing of Pseudoperonospora cubensis Reveals Effector Proteins with a QXLR Translocation Motif |
| title_full_unstemmed | 454 Genome Sequencing of Pseudoperonospora cubensis Reveals Effector Proteins with a QXLR Translocation Motif |
| title_short | 454 Genome Sequencing of Pseudoperonospora cubensis Reveals Effector Proteins with a QXLR Translocation Motif |
| title_sort | 454 genome sequencing of pseudoperonospora cubensis reveals effector proteins with a qxlr translocation motif |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-10-0185 |
| work_keys_str_mv | AT miaoyingtian 454genomesequencingofpseudoperonosporacubensisrevealseffectorproteinswithaqxlrtranslocationmotif AT joewin 454genomesequencingofpseudoperonosporacubensisrevealseffectorproteinswithaqxlrtranslocationmotif AT elizabethsavory 454genomesequencingofpseudoperonosporacubensisrevealseffectorproteinswithaqxlrtranslocationmotif AT alyssaburkhardt 454genomesequencingofpseudoperonosporacubensisrevealseffectorproteinswithaqxlrtranslocationmotif AT michaelheld 454genomesequencingofpseudoperonosporacubensisrevealseffectorproteinswithaqxlrtranslocationmotif AT federicabrandizzi 454genomesequencingofpseudoperonosporacubensisrevealseffectorproteinswithaqxlrtranslocationmotif AT bradday 454genomesequencingofpseudoperonosporacubensisrevealseffectorproteinswithaqxlrtranslocationmotif |