Sepsityper<sup>®</sup> Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry
In order to further accelerate pathogen identification from positive blood cultures (BC), various sample preparation protocols to identify bacteria with MALDI-TOF MS directly from positive BCs have been developed. We evaluated an in-house method in comparison to the Sepsityper<sup>®</sup>...
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MDPI AG
2022-10-01
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| author | Gabrijela Perše Ivana Samošćanec Zrinka Bošnjak Ana Budimir Tomislav Kuliš Ivana Mareković |
| author_facet | Gabrijela Perše Ivana Samošćanec Zrinka Bošnjak Ana Budimir Tomislav Kuliš Ivana Mareković |
| author_sort | Gabrijela Perše |
| collection | DOAJ |
| description | In order to further accelerate pathogen identification from positive blood cultures (BC), various sample preparation protocols to identify bacteria with MALDI-TOF MS directly from positive BCs have been developed. We evaluated an in-house method in comparison to the Sepsityper<sup>®</sup> Kit (Bruker Daltonics, Bremen, Germany) as well as the benefit of an on-plate formic acid extraction step following positive signal by the BACTEC<sup>TM</sup> FX system. Confirmation of identification was achieved using subcultured growing biomass used for MALDI-TOF MS analysis. A total of 113 monomicrobial positive BCs were analyzed. The rates of Gram-positive bacteria correctly identified to the genus level using in-house method and Sepsityper<sup>®</sup> Kit were 63.3% (38/60) and 81.7% (49/60), respectively (<i>p</i> = 0.025). Identification rates at species level for Gram-positive bacteria with in-house method and Sepsityper<sup>®</sup> kit were 30.0% (18/60) and 66.7% (40/60), respectively (<i>p</i> < 0.001). Identification rates of Gram-negative bacteria were similar with the in-house method and Sepsityper<sup>®</sup> Kit. Additional on-plate formic acid extraction demonstrated significant improvement in the identification rate of Gram-positive bacteria at both genus and species level for both in-house (<i>p</i> = 0.001, <i>p</i> < 0.001) and Sepsityper<sup>®</sup> Kit methods (<i>p</i> = 0.007, <i>p</i> < 0.001). Our in-house method is a candidate for laboratory routines with Sepsityper<sup>®</sup> Kit as a back-up solution when identification of Gram-positive bacteria is unsuccessful. |
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| language | English |
| last_indexed | 2024-03-09T18:55:51Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-8726b460ae524e3fa2ee5e024078f6f22023-11-24T05:30:15ZengMDPI AGLife2075-17292022-10-011211174410.3390/life12111744Sepsityper<sup>®</sup> Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass SpectrometryGabrijela Perše0Ivana Samošćanec1Zrinka Bošnjak2Ana Budimir3Tomislav Kuliš4Ivana Mareković5Department of Clinical and Molecular Microbiology, University Hospital Centre Zagreb, 10 000 Zagreb, CroatiaDepartment of Clinical Microbiology and Hospital Infections, University Hospital “Sveti Duh”, 10 000 Zagreb, CroatiaDepartment of Clinical and Molecular Microbiology, University Hospital Centre Zagreb, 10 000 Zagreb, CroatiaDepartment of Clinical and Molecular Microbiology, University Hospital Centre Zagreb, 10 000 Zagreb, CroatiaDepartment of Urology, University Hospital Centre Zagreb, 10 000 Zagreb, CroatiaDepartment of Clinical and Molecular Microbiology, University Hospital Centre Zagreb, 10 000 Zagreb, CroatiaIn order to further accelerate pathogen identification from positive blood cultures (BC), various sample preparation protocols to identify bacteria with MALDI-TOF MS directly from positive BCs have been developed. We evaluated an in-house method in comparison to the Sepsityper<sup>®</sup> Kit (Bruker Daltonics, Bremen, Germany) as well as the benefit of an on-plate formic acid extraction step following positive signal by the BACTEC<sup>TM</sup> FX system. Confirmation of identification was achieved using subcultured growing biomass used for MALDI-TOF MS analysis. A total of 113 monomicrobial positive BCs were analyzed. The rates of Gram-positive bacteria correctly identified to the genus level using in-house method and Sepsityper<sup>®</sup> Kit were 63.3% (38/60) and 81.7% (49/60), respectively (<i>p</i> = 0.025). Identification rates at species level for Gram-positive bacteria with in-house method and Sepsityper<sup>®</sup> kit were 30.0% (18/60) and 66.7% (40/60), respectively (<i>p</i> < 0.001). Identification rates of Gram-negative bacteria were similar with the in-house method and Sepsityper<sup>®</sup> Kit. Additional on-plate formic acid extraction demonstrated significant improvement in the identification rate of Gram-positive bacteria at both genus and species level for both in-house (<i>p</i> = 0.001, <i>p</i> < 0.001) and Sepsityper<sup>®</sup> Kit methods (<i>p</i> = 0.007, <i>p</i> < 0.001). Our in-house method is a candidate for laboratory routines with Sepsityper<sup>®</sup> Kit as a back-up solution when identification of Gram-positive bacteria is unsuccessful.https://www.mdpi.com/2075-1729/12/11/1744MALDI-TOF MSblood cultureidentificationin-house methodSepsityper<sup>®</sup> Kit |
| spellingShingle | Gabrijela Perše Ivana Samošćanec Zrinka Bošnjak Ana Budimir Tomislav Kuliš Ivana Mareković Sepsityper<sup>®</sup> Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry Life MALDI-TOF MS blood culture identification in-house method Sepsityper<sup>®</sup> Kit |
| title | Sepsityper<sup>®</sup> Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry |
| title_full | Sepsityper<sup>®</sup> Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry |
| title_fullStr | Sepsityper<sup>®</sup> Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry |
| title_full_unstemmed | Sepsityper<sup>®</sup> Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry |
| title_short | Sepsityper<sup>®</sup> Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry |
| title_sort | sepsityper sup r sup kit versus in house method in rapid identification of bacteria from positive blood cultures by maldi tof mass spectrometry |
| topic | MALDI-TOF MS blood culture identification in-house method Sepsityper<sup>®</sup> Kit |
| url | https://www.mdpi.com/2075-1729/12/11/1744 |
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