Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide
Background and Aim: The principal cytokines released by the host on infection include pro-inflammatory cytokines such as interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF). These cytokines were regarded as regulators of the host's response to infection. This study aimed to determine the...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-04-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.15/April-2022/9.pdf |
| _version_ | 1817997647735160832 |
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| author | Jatuporn Rattanasrisomporn Chananphat Tantikositruj Anyarat Thiptara Warangkana Kitpipit Ittidet Wichianrat Autchara Kayan Chaiwat Boonkaewwan |
| author_facet | Jatuporn Rattanasrisomporn Chananphat Tantikositruj Anyarat Thiptara Warangkana Kitpipit Ittidet Wichianrat Autchara Kayan Chaiwat Boonkaewwan |
| author_sort | Jatuporn Rattanasrisomporn |
| collection | DOAJ |
| description | Background and Aim: The principal cytokines released by the host on infection include pro-inflammatory cytokines such as interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF). These cytokines were regarded as regulators of the host's response to infection. This study aimed to determine the release of pro-inflammatory cytokines from chicken peripheral blood mononuclear cells (PBMCs) following lipopolysaccharide (LPS) stimulation.
Materials and Methods: Blood samples were collected from six Betong chickens. To isolate PBMCs, density gradient centrifugation was utilized. PBMC culture in RPMI1640 with 10% fetal bovine serum was stimulated with various concentrations of LPS (0, 0.01, 0.1, and 1.0 μg/mL). The production of TNF-α, IL-1β, and IL-6 was determined using an enzyme-linked immunosorbent assay.
Results: When the PBMCs were cultured for 24 h with varying doses of LPS, there was no significant variation in cell viability. TNF-α, IL-1β, and IL-6 levels were measured in Betong chicken PBMC. The release of these cytokines increased considerably as LPS concentration (0.01-1 μg/mL) increased (p<0.05).
Conclusion: In vitro studies of the chicken immune response, notably the release of pro-inflammatory cytokines, can be conducted using PBMCs obtained from chicken blood. |
| first_indexed | 2024-04-14T02:41:28Z |
| format | Article |
| id | doaj.art-87296604adfc46529fee9f6ba60fbb16 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-14T02:41:28Z |
| publishDate | 2022-04-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-87296604adfc46529fee9f6ba60fbb162022-12-22T02:17:03ZengVeterinary WorldVeterinary World0972-89882231-09162022-04-0115488588910.14202/vetworld.2022.885-889Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharideJatuporn Rattanasrisomporn0https://orcid.org/0000-0002-2426-0961Chananphat Tantikositruj1Anyarat Thiptara2Warangkana Kitpipit3https://orcid.org/0000-0003-4621-295XIttidet Wichianrat4Autchara Kayan5https://orcid.org/0000-0003-3481-8970Chaiwat Boonkaewwan6https://orcid.org/0000-0002-5390-632XDepartment of Companion Animal Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.Department of Animal Science, Kasetsart University, Bangkok 10900, Thailand.Department of Livestock Development, Veterinary Research and Development Center (Upper Southern Region), Nakhon Si Thammarat 80110, Thailand.Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand. Food Technology and Innovation Center of Excellence, Walailak University, Nakhon Si Thammarat, 80160, Thailand.Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand.Department of Animal Science, Kasetsart University, Bangkok 10900, Thailand.Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand.Background and Aim: The principal cytokines released by the host on infection include pro-inflammatory cytokines such as interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF). These cytokines were regarded as regulators of the host's response to infection. This study aimed to determine the release of pro-inflammatory cytokines from chicken peripheral blood mononuclear cells (PBMCs) following lipopolysaccharide (LPS) stimulation. Materials and Methods: Blood samples were collected from six Betong chickens. To isolate PBMCs, density gradient centrifugation was utilized. PBMC culture in RPMI1640 with 10% fetal bovine serum was stimulated with various concentrations of LPS (0, 0.01, 0.1, and 1.0 μg/mL). The production of TNF-α, IL-1β, and IL-6 was determined using an enzyme-linked immunosorbent assay. Results: When the PBMCs were cultured for 24 h with varying doses of LPS, there was no significant variation in cell viability. TNF-α, IL-1β, and IL-6 levels were measured in Betong chicken PBMC. The release of these cytokines increased considerably as LPS concentration (0.01-1 μg/mL) increased (p<0.05). Conclusion: In vitro studies of the chicken immune response, notably the release of pro-inflammatory cytokines, can be conducted using PBMCs obtained from chicken blood.http://www.veterinaryworld.org/Vol.15/April-2022/9.pdfchickeninterleukin-1βinterleukin-6peripheral blood mononuclear celltumor necrosis factor-α |
| spellingShingle | Jatuporn Rattanasrisomporn Chananphat Tantikositruj Anyarat Thiptara Warangkana Kitpipit Ittidet Wichianrat Autchara Kayan Chaiwat Boonkaewwan Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide Veterinary World chicken interleukin-1β interleukin-6 peripheral blood mononuclear cell tumor necrosis factor-α |
| title | Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide |
| title_full | Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide |
| title_fullStr | Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide |
| title_full_unstemmed | Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide |
| title_short | Pro-inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide |
| title_sort | pro inflammatory cytokine release from chicken peripheral blood mononuclear cells stimulated with lipopolysaccharide |
| topic | chicken interleukin-1β interleukin-6 peripheral blood mononuclear cell tumor necrosis factor-α |
| url | http://www.veterinaryworld.org/Vol.15/April-2022/9.pdf |
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