Treatment efficacy of low-dose 5-fluorouracil with ultrasound in mediating 5-fluorouracil-loaded microbubble cavitation in head and neck cancer
AbstractOver the past 50 years, 5-fluorouracil (5-FU) has played a critical role in the systemic chemotherapy of cancer patients. Bolus intravenous (IV) 5-FU infusion has been used due to the limitation of its extremely short half-life (10–15 min). This study used ultrasound (US) mediating 5-FU-load...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Drug Delivery |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10717544.2022.2154410 |
| _version_ | 1797260950274834432 |
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| author | Ai-Ho Liao Yu-An Lee Dao-Lung Lin Ho-Chiao Chuang Jehng-Kang Wang Ching-En Chang Hsiang-Tzu Li Ting-Yi Wu Cheng-Ping Shih Chih-Hung Wang Yueng-Hsiang Chu |
| author_facet | Ai-Ho Liao Yu-An Lee Dao-Lung Lin Ho-Chiao Chuang Jehng-Kang Wang Ching-En Chang Hsiang-Tzu Li Ting-Yi Wu Cheng-Ping Shih Chih-Hung Wang Yueng-Hsiang Chu |
| author_sort | Ai-Ho Liao |
| collection | DOAJ |
| description | AbstractOver the past 50 years, 5-fluorouracil (5-FU) has played a critical role in the systemic chemotherapy of cancer patients. Bolus intravenous (IV) 5-FU infusion has been used due to the limitation of its extremely short half-life (10–15 min). This study used ultrasound (US) mediating 5-FU-loaded microbubbles (MBs) cavitation as a tool to increase local intratumoral 5-FU levels with a reduced dose of 5-FU (a single IV injection of 2.5 mg/kg instead of a single intraperitoneal injection of 25–200 mg/kg as used in previous studies in mice). The 5-FU-MBs were prepared with a 132 mg/mL albumin solution and a 0.30 mg/mL 5-FU solution. The diameters of the MBs and 5-FU-MBs were 1.24 ± 0.85 and 2.00 ± 0.53 µm (mean ± SEM), respectively, and the maximum loading efficiency of 5-FU on MBs was 19.04 ± 0.25%. In the in vitro study, the cell viabilities of 5-FU and 5-FU-MBs did not differ significantly, but compared with the 5-FU-MBs treatment-alone group, cell toxicity increased to 31% in the 5-FU-MBs + US group (p < 0.001). The biodistribution results indicated that the 5-FU levels of the tumors in small animals were significant higher for the 5-FU-MBs + US treatment than for either the 5-FU-MBs or 5-FU treatment with low 5-FU systemic treatment doses (2.5 mg/kg 5-FU IV). In small-animal treatment, 2.5 mg/kg 5-FU therapeutic IV doses injected into mice caused a more-significant reduction in tumor growth in the 5-FU-MBs + US group (65.9%) than in the control group after 34 days of treatment. |
| first_indexed | 2024-03-08T23:30:48Z |
| format | Article |
| id | doaj.art-872d9d03bf354cda85d87c19c1c81525 |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-04-24T23:33:28Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-872d9d03bf354cda85d87c19c1c815252024-03-15T14:22:18ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642023-12-0130111310.1080/10717544.2022.2154410Treatment efficacy of low-dose 5-fluorouracil with ultrasound in mediating 5-fluorouracil-loaded microbubble cavitation in head and neck cancerAi-Ho Liao0Yu-An Lee1Dao-Lung Lin2Ho-Chiao Chuang3Jehng-Kang Wang4Ching-En Chang5Hsiang-Tzu Li6Ting-Yi Wu7Cheng-Ping Shih8Chih-Hung Wang9Yueng-Hsiang Chu10Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanGraduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanSpirit Scientific Co., Ltd, Taiwan Branch (Cayman), New Taipei City, TaiwanDepartment of Mechanical Engineering, National Taipei University of Technology, Taipei, TaiwanDepartment of Biochemistry, National Defense Medical Center, Taipei, TaiwanGraduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanGraduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanGraduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, TaiwanDepartment of Otolaryngology–Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, TaiwanDepartment of Otolaryngology–Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, TaiwanDepartment of Otolaryngology–Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, TaiwanAbstractOver the past 50 years, 5-fluorouracil (5-FU) has played a critical role in the systemic chemotherapy of cancer patients. Bolus intravenous (IV) 5-FU infusion has been used due to the limitation of its extremely short half-life (10–15 min). This study used ultrasound (US) mediating 5-FU-loaded microbubbles (MBs) cavitation as a tool to increase local intratumoral 5-FU levels with a reduced dose of 5-FU (a single IV injection of 2.5 mg/kg instead of a single intraperitoneal injection of 25–200 mg/kg as used in previous studies in mice). The 5-FU-MBs were prepared with a 132 mg/mL albumin solution and a 0.30 mg/mL 5-FU solution. The diameters of the MBs and 5-FU-MBs were 1.24 ± 0.85 and 2.00 ± 0.53 µm (mean ± SEM), respectively, and the maximum loading efficiency of 5-FU on MBs was 19.04 ± 0.25%. In the in vitro study, the cell viabilities of 5-FU and 5-FU-MBs did not differ significantly, but compared with the 5-FU-MBs treatment-alone group, cell toxicity increased to 31% in the 5-FU-MBs + US group (p < 0.001). The biodistribution results indicated that the 5-FU levels of the tumors in small animals were significant higher for the 5-FU-MBs + US treatment than for either the 5-FU-MBs or 5-FU treatment with low 5-FU systemic treatment doses (2.5 mg/kg 5-FU IV). In small-animal treatment, 2.5 mg/kg 5-FU therapeutic IV doses injected into mice caused a more-significant reduction in tumor growth in the 5-FU-MBs + US group (65.9%) than in the control group after 34 days of treatment.https://www.tandfonline.com/doi/10.1080/10717544.2022.2154410Microbubblesultrasoundhalf-life5-fluorouracilhead nd neck cancercavitation |
| spellingShingle | Ai-Ho Liao Yu-An Lee Dao-Lung Lin Ho-Chiao Chuang Jehng-Kang Wang Ching-En Chang Hsiang-Tzu Li Ting-Yi Wu Cheng-Ping Shih Chih-Hung Wang Yueng-Hsiang Chu Treatment efficacy of low-dose 5-fluorouracil with ultrasound in mediating 5-fluorouracil-loaded microbubble cavitation in head and neck cancer Drug Delivery Microbubbles ultrasound half-life 5-fluorouracil head nd neck cancer cavitation |
| title | Treatment efficacy of low-dose 5-fluorouracil with ultrasound in mediating 5-fluorouracil-loaded microbubble cavitation in head and neck cancer |
| title_full | Treatment efficacy of low-dose 5-fluorouracil with ultrasound in mediating 5-fluorouracil-loaded microbubble cavitation in head and neck cancer |
| title_fullStr | Treatment efficacy of low-dose 5-fluorouracil with ultrasound in mediating 5-fluorouracil-loaded microbubble cavitation in head and neck cancer |
| title_full_unstemmed | Treatment efficacy of low-dose 5-fluorouracil with ultrasound in mediating 5-fluorouracil-loaded microbubble cavitation in head and neck cancer |
| title_short | Treatment efficacy of low-dose 5-fluorouracil with ultrasound in mediating 5-fluorouracil-loaded microbubble cavitation in head and neck cancer |
| title_sort | treatment efficacy of low dose 5 fluorouracil with ultrasound in mediating 5 fluorouracil loaded microbubble cavitation in head and neck cancer |
| topic | Microbubbles ultrasound half-life 5-fluorouracil head nd neck cancer cavitation |
| url | https://www.tandfonline.com/doi/10.1080/10717544.2022.2154410 |
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