Protective Effects of N-Acetylcysteine on Lipopolysaccharide-Induced Respiratory Inflammation and Oxidative Stress
As the leading cause of bovine respiratory disease (BRD), bacterial pneumonia can result in tremendous losses in the herd farming industry worldwide. N-acetylcysteine (NAC), an acetylated precursor of the amino acid L-cysteine, has been reported to have anti-inflammatory and antioxidant properties....
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/11/5/879 |
| _version_ | 1797501989362335744 |
|---|---|
| author | Hongbai Chen Nana Ma Xiaokun Song Guozhen Wei Hongzhu Zhang Jing Liu Xiangzhen Shen Xiangkai Zhuge Guangjun Chang |
| author_facet | Hongbai Chen Nana Ma Xiaokun Song Guozhen Wei Hongzhu Zhang Jing Liu Xiangzhen Shen Xiangkai Zhuge Guangjun Chang |
| author_sort | Hongbai Chen |
| collection | DOAJ |
| description | As the leading cause of bovine respiratory disease (BRD), bacterial pneumonia can result in tremendous losses in the herd farming industry worldwide. N-acetylcysteine (NAC), an acetylated precursor of the amino acid L-cysteine, has been reported to have anti-inflammatory and antioxidant properties. To explore the protective effect and underlying mechanisms of NAC in ALI, we investigated its role in lipopolysaccharide (LPS)-induced bovine embryo tracheal cells (EBTr) and mouse lung injury models. We found that NAC pretreatment attenuated LPS-induced inflammation in EBTr and mouse models. Moreover, LPS suppressed the expression of oxidative-related factors in EBTr and promoted gene expression and the secretion of inflammatory cytokines. Conversely, the pretreatment of NAC alleviated the secretion of inflammatory cytokines and decreased their mRNA levels, maintaining stable levels of antioxidative gene expression. In vivo, NAC helped LPS-induced inflammatory responses and lung injury in ALI mice. The relative protein concentration, total cells, and percentage of neutrophils in BALF; the level of secretion of IL-6, IL-8, TNF-α, and IL-1β; MPO activity; lung injury score; and the expression level of inflammatory-related genes were decreased significantly in the NAC group compared with the LPS group. NAC also ameliorated LPS-induced mRNA level changes in antioxidative genes. In conclusion, our findings suggest that NAC affects the inflammatory and oxidative response, alleviating LPS-induced EBTr inflammation and mouse lung injury, which offers a natural therapeutic strategy for BRD. |
| first_indexed | 2024-03-10T03:26:26Z |
| format | Article |
| id | doaj.art-872e7e13b9144a7a94300b60183abd93 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:26:26Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-872e7e13b9144a7a94300b60183abd932023-11-23T09:51:09ZengMDPI AGAntioxidants2076-39212022-04-0111587910.3390/antiox11050879Protective Effects of N-Acetylcysteine on Lipopolysaccharide-Induced Respiratory Inflammation and Oxidative StressHongbai Chen0Nana Ma1Xiaokun Song2Guozhen Wei3Hongzhu Zhang4Jing Liu5Xiangzhen Shen6Xiangkai Zhuge7Guangjun Chang8Ministry of Education Joint International Research, Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaMinistry of Education Joint International Research, Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaMinistry of Education Joint International Research, Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaMinistry of Education Joint International Research, Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaMinistry of Education Joint International Research, Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaCollege of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, ChinaMinistry of Education Joint International Research, Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Nantong University, Nantong 226019, ChinaMinistry of Education Joint International Research, Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaAs the leading cause of bovine respiratory disease (BRD), bacterial pneumonia can result in tremendous losses in the herd farming industry worldwide. N-acetylcysteine (NAC), an acetylated precursor of the amino acid L-cysteine, has been reported to have anti-inflammatory and antioxidant properties. To explore the protective effect and underlying mechanisms of NAC in ALI, we investigated its role in lipopolysaccharide (LPS)-induced bovine embryo tracheal cells (EBTr) and mouse lung injury models. We found that NAC pretreatment attenuated LPS-induced inflammation in EBTr and mouse models. Moreover, LPS suppressed the expression of oxidative-related factors in EBTr and promoted gene expression and the secretion of inflammatory cytokines. Conversely, the pretreatment of NAC alleviated the secretion of inflammatory cytokines and decreased their mRNA levels, maintaining stable levels of antioxidative gene expression. In vivo, NAC helped LPS-induced inflammatory responses and lung injury in ALI mice. The relative protein concentration, total cells, and percentage of neutrophils in BALF; the level of secretion of IL-6, IL-8, TNF-α, and IL-1β; MPO activity; lung injury score; and the expression level of inflammatory-related genes were decreased significantly in the NAC group compared with the LPS group. NAC also ameliorated LPS-induced mRNA level changes in antioxidative genes. In conclusion, our findings suggest that NAC affects the inflammatory and oxidative response, alleviating LPS-induced EBTr inflammation and mouse lung injury, which offers a natural therapeutic strategy for BRD.https://www.mdpi.com/2076-3921/11/5/879N-acetylcysteinelipopolysaccharide (LPS)acute lung injury (ALI)bovine respiratory disease (BRD)EBTrinflammation |
| spellingShingle | Hongbai Chen Nana Ma Xiaokun Song Guozhen Wei Hongzhu Zhang Jing Liu Xiangzhen Shen Xiangkai Zhuge Guangjun Chang Protective Effects of N-Acetylcysteine on Lipopolysaccharide-Induced Respiratory Inflammation and Oxidative Stress Antioxidants N-acetylcysteine lipopolysaccharide (LPS) acute lung injury (ALI) bovine respiratory disease (BRD) EBTr inflammation |
| title | Protective Effects of N-Acetylcysteine on Lipopolysaccharide-Induced Respiratory Inflammation and Oxidative Stress |
| title_full | Protective Effects of N-Acetylcysteine on Lipopolysaccharide-Induced Respiratory Inflammation and Oxidative Stress |
| title_fullStr | Protective Effects of N-Acetylcysteine on Lipopolysaccharide-Induced Respiratory Inflammation and Oxidative Stress |
| title_full_unstemmed | Protective Effects of N-Acetylcysteine on Lipopolysaccharide-Induced Respiratory Inflammation and Oxidative Stress |
| title_short | Protective Effects of N-Acetylcysteine on Lipopolysaccharide-Induced Respiratory Inflammation and Oxidative Stress |
| title_sort | protective effects of n acetylcysteine on lipopolysaccharide induced respiratory inflammation and oxidative stress |
| topic | N-acetylcysteine lipopolysaccharide (LPS) acute lung injury (ALI) bovine respiratory disease (BRD) EBTr inflammation |
| url | https://www.mdpi.com/2076-3921/11/5/879 |
| work_keys_str_mv | AT hongbaichen protectiveeffectsofnacetylcysteineonlipopolysaccharideinducedrespiratoryinflammationandoxidativestress AT nanama protectiveeffectsofnacetylcysteineonlipopolysaccharideinducedrespiratoryinflammationandoxidativestress AT xiaokunsong protectiveeffectsofnacetylcysteineonlipopolysaccharideinducedrespiratoryinflammationandoxidativestress AT guozhenwei protectiveeffectsofnacetylcysteineonlipopolysaccharideinducedrespiratoryinflammationandoxidativestress AT hongzhuzhang protectiveeffectsofnacetylcysteineonlipopolysaccharideinducedrespiratoryinflammationandoxidativestress AT jingliu protectiveeffectsofnacetylcysteineonlipopolysaccharideinducedrespiratoryinflammationandoxidativestress AT xiangzhenshen protectiveeffectsofnacetylcysteineonlipopolysaccharideinducedrespiratoryinflammationandoxidativestress AT xiangkaizhuge protectiveeffectsofnacetylcysteineonlipopolysaccharideinducedrespiratoryinflammationandoxidativestress AT guangjunchang protectiveeffectsofnacetylcysteineonlipopolysaccharideinducedrespiratoryinflammationandoxidativestress |