Downregulation of lncRNA EPB41L4A-AS1 promotes gastric cancer cell proliferation, migration and invasion
Abstract Background The incidence of gastric cancer ranks the first among digestive tract tumors in China. However, there are no specific symptoms in the early stage of the tumor and the diagnosis process is complex, so more effective detection methods are very needed. In this study, a novel long no...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Gastroenterology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12876-024-03216-9 |
| _version_ | 1797199408470687744 |
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| author | Jiancang Ma Yingying Feng Jinkai Xu Zongyu Li Jingyue Lai Hao Guan |
| author_facet | Jiancang Ma Yingying Feng Jinkai Xu Zongyu Li Jingyue Lai Hao Guan |
| author_sort | Jiancang Ma |
| collection | DOAJ |
| description | Abstract Background The incidence of gastric cancer ranks the first among digestive tract tumors in China. However, there are no specific symptoms in the early stage of the tumor and the diagnosis process is complex, so more effective detection methods are very needed. In this study, a novel long noncoding RNA (lncRNA) was introduced as a diagnostic biomarker for gastric cancer, which brought new thinking to the exploration of its pathological mechanism and clinical prediction. Methods The level of lncRNA EPB41L4A-AS1 (EPB41L4A-AS1) in gastric cancer serum and cells was verified via real-time quantitative polymerase chain reaction (RT-qPCR). Receiver operating characteristic (ROC) curve was performed based on the EPB41L4A-AS1 level, and the diagnostic possibility of EPB41L4A-AS was analyzed. The chi-square test evaluated the correlation between EPB41L4A-AS expression and clinical information. The cells were cultured and transfected in vitro, and the mediations of abnormal EPB41L4A-AS level on the viability and motility of gastric cancer cells were verified through cell counting kit-8 (CCK-8) and Transwell assay. Furthermore, luciferase activity assay was performed to confirm the sponge molecule microRNA-17-5p (miR-17-5p) of EPB41L4A-AS1. Results EPB41L4A-AS1 was decreased in gastric cancer, and low EPB41L4A-AS1 level indicated resultful diagnostic value. Overexpression of EPB41L4A-AS1 inhibited the activity of gastric cancer cells, while knockdown of EPB41L4A-AS1 promoted tumor deterioration. EPB41L4A-AS1 directly targeted and regulated the expression ofmiR-17-5p. Conclusion This study elaborated that EPB41L4A-AS1 is lowly expressed in gastric cancer. Silencing EPB41L4A-AS1 was beneficial to cell proliferation, migration, and invasion. EPB41L4A-AS1 provides a new possibility for the diagnosis of gastric cancer patients by targeting miR-17-5p. |
| first_indexed | 2024-04-24T07:15:17Z |
| format | Article |
| id | doaj.art-873405375eb7414691e54b9e22538444 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-04-24T07:15:17Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-873405375eb7414691e54b9e225384442024-04-21T11:20:44ZengBMCBMC Gastroenterology1471-230X2024-04-012411810.1186/s12876-024-03216-9Downregulation of lncRNA EPB41L4A-AS1 promotes gastric cancer cell proliferation, migration and invasionJiancang Ma0Yingying Feng1Jinkai Xu2Zongyu Li3Jingyue Lai4Hao Guan5Department of Vascular Surgery, The Second Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Pathophysiology, Obesity and Diabetes Research Center, Navy Medical UniversityDepartment of Vascular Surgery, The Second Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Vascular Surgery, The Second Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Vascular Surgery, The Second Affiliated Hospital of Xi’an Jiaotong UniversityDepartment of Vascular Surgery, The Second Affiliated Hospital of Xi’an Jiaotong UniversityAbstract Background The incidence of gastric cancer ranks the first among digestive tract tumors in China. However, there are no specific symptoms in the early stage of the tumor and the diagnosis process is complex, so more effective detection methods are very needed. In this study, a novel long noncoding RNA (lncRNA) was introduced as a diagnostic biomarker for gastric cancer, which brought new thinking to the exploration of its pathological mechanism and clinical prediction. Methods The level of lncRNA EPB41L4A-AS1 (EPB41L4A-AS1) in gastric cancer serum and cells was verified via real-time quantitative polymerase chain reaction (RT-qPCR). Receiver operating characteristic (ROC) curve was performed based on the EPB41L4A-AS1 level, and the diagnostic possibility of EPB41L4A-AS was analyzed. The chi-square test evaluated the correlation between EPB41L4A-AS expression and clinical information. The cells were cultured and transfected in vitro, and the mediations of abnormal EPB41L4A-AS level on the viability and motility of gastric cancer cells were verified through cell counting kit-8 (CCK-8) and Transwell assay. Furthermore, luciferase activity assay was performed to confirm the sponge molecule microRNA-17-5p (miR-17-5p) of EPB41L4A-AS1. Results EPB41L4A-AS1 was decreased in gastric cancer, and low EPB41L4A-AS1 level indicated resultful diagnostic value. Overexpression of EPB41L4A-AS1 inhibited the activity of gastric cancer cells, while knockdown of EPB41L4A-AS1 promoted tumor deterioration. EPB41L4A-AS1 directly targeted and regulated the expression ofmiR-17-5p. Conclusion This study elaborated that EPB41L4A-AS1 is lowly expressed in gastric cancer. Silencing EPB41L4A-AS1 was beneficial to cell proliferation, migration, and invasion. EPB41L4A-AS1 provides a new possibility for the diagnosis of gastric cancer patients by targeting miR-17-5p.https://doi.org/10.1186/s12876-024-03216-9Gastric cancerEPB41L4A-AS1DiagnosismiR-17-5pProgression |
| spellingShingle | Jiancang Ma Yingying Feng Jinkai Xu Zongyu Li Jingyue Lai Hao Guan Downregulation of lncRNA EPB41L4A-AS1 promotes gastric cancer cell proliferation, migration and invasion BMC Gastroenterology Gastric cancer EPB41L4A-AS1 Diagnosis miR-17-5p Progression |
| title | Downregulation of lncRNA EPB41L4A-AS1 promotes gastric cancer cell proliferation, migration and invasion |
| title_full | Downregulation of lncRNA EPB41L4A-AS1 promotes gastric cancer cell proliferation, migration and invasion |
| title_fullStr | Downregulation of lncRNA EPB41L4A-AS1 promotes gastric cancer cell proliferation, migration and invasion |
| title_full_unstemmed | Downregulation of lncRNA EPB41L4A-AS1 promotes gastric cancer cell proliferation, migration and invasion |
| title_short | Downregulation of lncRNA EPB41L4A-AS1 promotes gastric cancer cell proliferation, migration and invasion |
| title_sort | downregulation of lncrna epb41l4a as1 promotes gastric cancer cell proliferation migration and invasion |
| topic | Gastric cancer EPB41L4A-AS1 Diagnosis miR-17-5p Progression |
| url | https://doi.org/10.1186/s12876-024-03216-9 |
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