Developmental Stage-Specific Distribution of Macrophages in Mouse Mammary Gland
Mammary gland development begins in the embryo and continues throughout the reproductive life of female mammals. Tissue macrophages (Mϕs), dependent on signals from the Mϕ colony stimulating factor 1 receptor (CSF1R), have been shown to regulate the generation, regression and regeneration of this or...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2019.00250/full |
| _version_ | 1818137717630828544 |
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| author | Teneale A. Stewart Teneale A. Stewart Katherine Hughes David A. Hume David A. Hume Felicity M. Davis Felicity M. Davis |
| author_facet | Teneale A. Stewart Teneale A. Stewart Katherine Hughes David A. Hume David A. Hume Felicity M. Davis Felicity M. Davis |
| author_sort | Teneale A. Stewart |
| collection | DOAJ |
| description | Mammary gland development begins in the embryo and continues throughout the reproductive life of female mammals. Tissue macrophages (Mϕs), dependent on signals from the Mϕ colony stimulating factor 1 receptor (CSF1R), have been shown to regulate the generation, regression and regeneration of this organ, which is central for mammalian offspring survival. However, the distribution of Mϕs in the pre- and post-natal mammary gland, as it undergoes distinct phases of development and regression, is unknown or has been inferred from immunostaining of thin tissue sections. Here, we used optical tissue clearing and 3-dimensional imaging of mammary tissue obtained from Csf1r-EGFP mice. Whilst tissue Mϕs were observed at all developmental phases, their abundance, morphology, localization and association with luminal and basal epithelial cells exhibited stage-specific differences. Furthermore, sexual dimorphism was observed at E14.5, when the male mammary bud is severed from the overlying epidermis. These findings provide new insights into the localization and possible functions of heterogeneous tissue Mϕ populations in mammogenesis. |
| first_indexed | 2024-12-11T10:00:44Z |
| format | Article |
| id | doaj.art-87379b517ea847749aaceb1eaad8a330 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-11T10:00:44Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-87379b517ea847749aaceb1eaad8a3302022-12-22T01:12:08ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2019-10-01710.3389/fcell.2019.00250493487Developmental Stage-Specific Distribution of Macrophages in Mouse Mammary GlandTeneale A. Stewart0Teneale A. Stewart1Katherine Hughes2David A. Hume3David A. Hume4Felicity M. Davis5Felicity M. Davis6Faculty of Medicine, Mater Research Institute-The University of Queensland, Brisbane, QLD, AustraliaTranslational Research Institute, Brisbane, QLD, AustraliaDepartment of Veterinary Medicine, University of Cambridge, Cambridge, United KingdomFaculty of Medicine, Mater Research Institute-The University of Queensland, Brisbane, QLD, AustraliaTranslational Research Institute, Brisbane, QLD, AustraliaFaculty of Medicine, Mater Research Institute-The University of Queensland, Brisbane, QLD, AustraliaTranslational Research Institute, Brisbane, QLD, AustraliaMammary gland development begins in the embryo and continues throughout the reproductive life of female mammals. Tissue macrophages (Mϕs), dependent on signals from the Mϕ colony stimulating factor 1 receptor (CSF1R), have been shown to regulate the generation, regression and regeneration of this organ, which is central for mammalian offspring survival. However, the distribution of Mϕs in the pre- and post-natal mammary gland, as it undergoes distinct phases of development and regression, is unknown or has been inferred from immunostaining of thin tissue sections. Here, we used optical tissue clearing and 3-dimensional imaging of mammary tissue obtained from Csf1r-EGFP mice. Whilst tissue Mϕs were observed at all developmental phases, their abundance, morphology, localization and association with luminal and basal epithelial cells exhibited stage-specific differences. Furthermore, sexual dimorphism was observed at E14.5, when the male mammary bud is severed from the overlying epidermis. These findings provide new insights into the localization and possible functions of heterogeneous tissue Mϕ populations in mammogenesis.https://www.frontiersin.org/article/10.3389/fcell.2019.00250/fullmammary glandmacrophagesdevelopmentembryonic mammary stem cellsadult mammary stem cellsstem cell niche |
| spellingShingle | Teneale A. Stewart Teneale A. Stewart Katherine Hughes David A. Hume David A. Hume Felicity M. Davis Felicity M. Davis Developmental Stage-Specific Distribution of Macrophages in Mouse Mammary Gland Frontiers in Cell and Developmental Biology mammary gland macrophages development embryonic mammary stem cells adult mammary stem cells stem cell niche |
| title | Developmental Stage-Specific Distribution of Macrophages in Mouse Mammary Gland |
| title_full | Developmental Stage-Specific Distribution of Macrophages in Mouse Mammary Gland |
| title_fullStr | Developmental Stage-Specific Distribution of Macrophages in Mouse Mammary Gland |
| title_full_unstemmed | Developmental Stage-Specific Distribution of Macrophages in Mouse Mammary Gland |
| title_short | Developmental Stage-Specific Distribution of Macrophages in Mouse Mammary Gland |
| title_sort | developmental stage specific distribution of macrophages in mouse mammary gland |
| topic | mammary gland macrophages development embryonic mammary stem cells adult mammary stem cells stem cell niche |
| url | https://www.frontiersin.org/article/10.3389/fcell.2019.00250/full |
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