Metagenomics reveals the variations in functional metabolism associated with greenhouse gas emissions during legume-vegetable rotation process
Legume-based rotation is commonly recognized for its mitigation efficiency of greenhouse gas (GHG) emissions. However, variations in GHG emission-associated metabolic functions during the legume-vegetable rotation process remain largely uncharacterized. Accordingly, a soybean-radish rotation field e...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324003440 |
| _version_ | 1797217110180495360 |
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| author | Xinxin You Sheng Wang Linna Du Yurong Chen Ting Wang Xiaoxu Bo |
| author_facet | Xinxin You Sheng Wang Linna Du Yurong Chen Ting Wang Xiaoxu Bo |
| author_sort | Xinxin You |
| collection | DOAJ |
| description | Legume-based rotation is commonly recognized for its mitigation efficiency of greenhouse gas (GHG) emissions. However, variations in GHG emission-associated metabolic functions during the legume-vegetable rotation process remain largely uncharacterized. Accordingly, a soybean-radish rotation field experiment was designed to clarify the responses of microbial communities and their GHG emission-associated functional metabolism through metagenomics. The results showed that the contents of soil organic carbon and total phosphorus significantly decreased during the soybean-radish process (P < 0.05), while soil total potassium content and bacterial richness and diversity significantly increased (P < 0.05). Moreover, the predominant bacterial phyla varied, with a decrease in the relative abundance of Proteobacteria and an increase in the relative abundance of Acidobacteria, Gemmatimonadetes, and Chloroflexi. Metagenomics clarified that bacterial carbohydrate metabolism substantially increased during the rotation process, whereas formaldehyde assimilation, methanogenesis, nitrification, and dissimilatory nitrate reduction decreased (P < 0.05). Specifically, the expression of phosphate acetyltransferase (functional methanogenesis gene, pta) and nitrate reductase gamma subunit (functional dissimilatory nitrate reduction gene, narI) was inhibited, indicating of low methane production and nitrogen metabolism. Additionally, the partial least squares path model revealed that the Shannon diversity index was negatively correlated with methane and nitrogen metabolism (P < 0.01), further demonstrating that the response of the soil bacterial microbiome responses are closely linked with GHG-associated metabolism during the soybean-radish rotation process. Collectively, our findings shed light on the responses of soil microbial communities to functional metabolism associated with GHG emissions and provide important insights to mitigate GHG emissions during the rotational cropping of legumes and vegetables. |
| first_indexed | 2024-04-24T11:56:38Z |
| format | Article |
| id | doaj.art-8737d96371c74de08ee1ddd547f96f2a |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-24T11:56:38Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-8737d96371c74de08ee1ddd547f96f2a2024-04-09T04:12:35ZengElsevierEcotoxicology and Environmental Safety0147-65132024-04-01275116268Metagenomics reveals the variations in functional metabolism associated with greenhouse gas emissions during legume-vegetable rotation processXinxin You0Sheng Wang1Linna Du2Yurong Chen3Ting Wang4Xiaoxu Bo5Institute of Eco-environmental Sciences, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, PR China; Southern Zhejiang Key Laboratory of Crop Breeding, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, PR ChinaInstitute of Eco-environmental Sciences, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, PR China; Correspondence to: Wenzhou Academy of Agricultural Sciences, Wenzhou, Zhejiang Province 325006, PR China.College of Advanced Materials Engineering, Jiaxing Nanhu University, Jiaxing 314001, PR China; Correspondence to: Jiaxing Nanhu University, Jiaxing, Zhejiang Province 314001, PR China.Institute of Eco-environmental Sciences, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, PR ChinaInstitute of Eco-environmental Sciences, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, PR ChinaInstitute of Eco-environmental Sciences, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, PR ChinaLegume-based rotation is commonly recognized for its mitigation efficiency of greenhouse gas (GHG) emissions. However, variations in GHG emission-associated metabolic functions during the legume-vegetable rotation process remain largely uncharacterized. Accordingly, a soybean-radish rotation field experiment was designed to clarify the responses of microbial communities and their GHG emission-associated functional metabolism through metagenomics. The results showed that the contents of soil organic carbon and total phosphorus significantly decreased during the soybean-radish process (P < 0.05), while soil total potassium content and bacterial richness and diversity significantly increased (P < 0.05). Moreover, the predominant bacterial phyla varied, with a decrease in the relative abundance of Proteobacteria and an increase in the relative abundance of Acidobacteria, Gemmatimonadetes, and Chloroflexi. Metagenomics clarified that bacterial carbohydrate metabolism substantially increased during the rotation process, whereas formaldehyde assimilation, methanogenesis, nitrification, and dissimilatory nitrate reduction decreased (P < 0.05). Specifically, the expression of phosphate acetyltransferase (functional methanogenesis gene, pta) and nitrate reductase gamma subunit (functional dissimilatory nitrate reduction gene, narI) was inhibited, indicating of low methane production and nitrogen metabolism. Additionally, the partial least squares path model revealed that the Shannon diversity index was negatively correlated with methane and nitrogen metabolism (P < 0.01), further demonstrating that the response of the soil bacterial microbiome responses are closely linked with GHG-associated metabolism during the soybean-radish rotation process. Collectively, our findings shed light on the responses of soil microbial communities to functional metabolism associated with GHG emissions and provide important insights to mitigate GHG emissions during the rotational cropping of legumes and vegetables.http://www.sciencedirect.com/science/article/pii/S0147651324003440legume-vegetable rotationgreenhouse gas emissionsmetagenomicscarbohydrate metabolismmethane metabolismnitrogen metabolism |
| spellingShingle | Xinxin You Sheng Wang Linna Du Yurong Chen Ting Wang Xiaoxu Bo Metagenomics reveals the variations in functional metabolism associated with greenhouse gas emissions during legume-vegetable rotation process Ecotoxicology and Environmental Safety legume-vegetable rotation greenhouse gas emissions metagenomics carbohydrate metabolism methane metabolism nitrogen metabolism |
| title | Metagenomics reveals the variations in functional metabolism associated with greenhouse gas emissions during legume-vegetable rotation process |
| title_full | Metagenomics reveals the variations in functional metabolism associated with greenhouse gas emissions during legume-vegetable rotation process |
| title_fullStr | Metagenomics reveals the variations in functional metabolism associated with greenhouse gas emissions during legume-vegetable rotation process |
| title_full_unstemmed | Metagenomics reveals the variations in functional metabolism associated with greenhouse gas emissions during legume-vegetable rotation process |
| title_short | Metagenomics reveals the variations in functional metabolism associated with greenhouse gas emissions during legume-vegetable rotation process |
| title_sort | metagenomics reveals the variations in functional metabolism associated with greenhouse gas emissions during legume vegetable rotation process |
| topic | legume-vegetable rotation greenhouse gas emissions metagenomics carbohydrate metabolism methane metabolism nitrogen metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324003440 |
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