An Outbreak of Subclinical Mastitis in a Dairy Herd Caused by a Novel <i>Streptococcus canis</i> Sequence Type (ST55)
The present case report provides data on the phenotypic and genotypic properties of <i>S. canis</i> isolated from nine dairy cows with subclinical mastitis (SCC greater than 200,000 cells/mL in the quarter milk sample, no clinical signs) and from a cat living in the barn and reports the...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/11/2/550 |
| _version_ | 1797395989349269504 |
|---|---|
| author | Cassandra Eibl Martina Baumgartner Verena Urbantke Michael Sigmund Katharina Lichtmannsperger Thomas Wittek Joachim Spergser |
| author_facet | Cassandra Eibl Martina Baumgartner Verena Urbantke Michael Sigmund Katharina Lichtmannsperger Thomas Wittek Joachim Spergser |
| author_sort | Cassandra Eibl |
| collection | DOAJ |
| description | The present case report provides data on the phenotypic and genotypic properties of <i>S. canis</i> isolated from nine dairy cows with subclinical mastitis (SCC greater than 200,000 cells/mL in the quarter milk sample, no clinical signs) and from a cat living in the barn and reports the eradication of the pathogen from the herd with an automatic milking system. The isolates were identified using conventional bacteriology, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and genetic relationships were investigated by multilocus sequence typing (MLST). Udder health management and hygiene instructions comprised the removal of the carnivores from the barn, strict monitoring of milking hygiene and techniques to avoid new infections via the milking robot, with simultaneous therapy for all infected cows. Phenotypic and genotypic properties of all isolates were identical. MLST revealed a unique sequence type (ST55) and a farmyard cat was identified as the most likely source of the <i>S. canis</i> infection in cows. The simultaneous treatment of all infected cows and management and hygiene improvements lead to a decreased SCC within four weeks. |
| first_indexed | 2024-03-09T00:43:49Z |
| format | Article |
| id | doaj.art-8737fe71784148d582a5286405a00145 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T00:43:49Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-8737fe71784148d582a5286405a001452023-12-11T17:41:34ZengMDPI AGAnimals2076-26152021-02-0111255010.3390/ani11020550An Outbreak of Subclinical Mastitis in a Dairy Herd Caused by a Novel <i>Streptococcus canis</i> Sequence Type (ST55)Cassandra Eibl0Martina Baumgartner1Verena Urbantke2Michael Sigmund3Katharina Lichtmannsperger4Thomas Wittek5Joachim Spergser6University Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaUniversity Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaUniversity Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaUniversity Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaUniversity Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaUniversity Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaInstitute of Microbiology, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, AustriaThe present case report provides data on the phenotypic and genotypic properties of <i>S. canis</i> isolated from nine dairy cows with subclinical mastitis (SCC greater than 200,000 cells/mL in the quarter milk sample, no clinical signs) and from a cat living in the barn and reports the eradication of the pathogen from the herd with an automatic milking system. The isolates were identified using conventional bacteriology, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and genetic relationships were investigated by multilocus sequence typing (MLST). Udder health management and hygiene instructions comprised the removal of the carnivores from the barn, strict monitoring of milking hygiene and techniques to avoid new infections via the milking robot, with simultaneous therapy for all infected cows. Phenotypic and genotypic properties of all isolates were identical. MLST revealed a unique sequence type (ST55) and a farmyard cat was identified as the most likely source of the <i>S. canis</i> infection in cows. The simultaneous treatment of all infected cows and management and hygiene improvements lead to a decreased SCC within four weeks.https://www.mdpi.com/2076-2615/11/2/550β-hemolytic streptococcimastitismultilocus sequence typing<i>Streptococcus canis</i> ST55 |
| spellingShingle | Cassandra Eibl Martina Baumgartner Verena Urbantke Michael Sigmund Katharina Lichtmannsperger Thomas Wittek Joachim Spergser An Outbreak of Subclinical Mastitis in a Dairy Herd Caused by a Novel <i>Streptococcus canis</i> Sequence Type (ST55) Animals β-hemolytic streptococci mastitis multilocus sequence typing <i>Streptococcus canis</i> ST55 |
| title | An Outbreak of Subclinical Mastitis in a Dairy Herd Caused by a Novel <i>Streptococcus canis</i> Sequence Type (ST55) |
| title_full | An Outbreak of Subclinical Mastitis in a Dairy Herd Caused by a Novel <i>Streptococcus canis</i> Sequence Type (ST55) |
| title_fullStr | An Outbreak of Subclinical Mastitis in a Dairy Herd Caused by a Novel <i>Streptococcus canis</i> Sequence Type (ST55) |
| title_full_unstemmed | An Outbreak of Subclinical Mastitis in a Dairy Herd Caused by a Novel <i>Streptococcus canis</i> Sequence Type (ST55) |
| title_short | An Outbreak of Subclinical Mastitis in a Dairy Herd Caused by a Novel <i>Streptococcus canis</i> Sequence Type (ST55) |
| title_sort | outbreak of subclinical mastitis in a dairy herd caused by a novel i streptococcus canis i sequence type st55 |
| topic | β-hemolytic streptococci mastitis multilocus sequence typing <i>Streptococcus canis</i> ST55 |
| url | https://www.mdpi.com/2076-2615/11/2/550 |
| work_keys_str_mv | AT cassandraeibl anoutbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT martinabaumgartner anoutbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT verenaurbantke anoutbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT michaelsigmund anoutbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT katharinalichtmannsperger anoutbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT thomaswittek anoutbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT joachimspergser anoutbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT cassandraeibl outbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT martinabaumgartner outbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT verenaurbantke outbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT michaelsigmund outbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT katharinalichtmannsperger outbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT thomaswittek outbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 AT joachimspergser outbreakofsubclinicalmastitisinadairyherdcausedbyanovelistreptococcuscanisisequencetypest55 |