Changes and correlations of T-cell coinhibitory molecule programmed death-1 and interferon-γ in pediatric immune thrombocytopenia
Background Immune thrombocytopenia (ITP) is an acquired autoimmune disease characterized by abnormalities of T cells subsets. Programmed death-1 (PD-1) is a co-signaling inhibitory molecule in T cells that is involved in many autoimmune diseases. Purpose Here we aimed to measure changes in PD-1 expr...
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| Format: | Article |
| Language: | English |
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The Korean Pediatric Society
2023-03-01
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| Series: | Clinical and Experimental Pediatrics |
| Subjects: | |
| Online Access: | http://www.e-cep.org/upload/pdf/cep-2022-00920.pdf |
| _version_ | 1811159559989886976 |
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| author | Fady Mohamed El-Gendy Amira M.F. Shehata Esam Awad Abd El-Kawy Mahmoud Ahmed El-Hawy |
| author_facet | Fady Mohamed El-Gendy Amira M.F. Shehata Esam Awad Abd El-Kawy Mahmoud Ahmed El-Hawy |
| author_sort | Fady Mohamed El-Gendy |
| collection | DOAJ |
| description | Background Immune thrombocytopenia (ITP) is an acquired autoimmune disease characterized by abnormalities of T cells subsets. Programmed death-1 (PD-1) is a co-signaling inhibitory molecule in T cells that is involved in many autoimmune diseases. Purpose Here we aimed to measure changes in PD-1 expression and serum interferon-γ (IFN-γ) levels before and 1 month after treatment in pediatric patients with newly diagnosed ITP. Methods We measured PD-1+ CD4+ T cells percentages using flow cytometry and the serum IFN-γ levels by enzyme-linked immunosorbent assay in 40 pediatric patients with ITP and 20 healthy controls. Results Compared with healthy controls, the PD-1+ CD4+ T cells percentages and serum IFN-γ levels were significantly higher in ITP patients before and 1 month after therapy. A correlation study revealed that PD-1+ CD4+ T cells percentage was negatively associated with platelet count and positively associated with IFN-γ level in patients with ITP. Furthermore, serum IFN-γ levels were significantly decreased in patients after treatment, but no significant change was detected in the percentage of PD-1+ CD4+ T cells before or 1 month after therapy. Conclusion PD-1+ CD4+ T cells expression and IFN-γ levels were increased in patients with ITP. These preliminary data suggest a potential role of PD-1+ CD4+ T cells as mediators of ITP. We also found a correlation between PD-1+ CD4+ T cells and both platelet counts and IFN-γ levels. These findings suggest a potential role of PD-1+ CD4+ T cells and IFN-γ in the pathogenesis of ITP. Further studies investigating PD-1 expression in different T-cell subsets, serum IFN-γ concentrations, and antiplatelet antibodies levels over a longer duration after therapy initiation could delineate the precise role of PD-1 in ITP pathogenesis. Consequently, novel nontraditional therapeutic strategies for ITP patients may become available. |
| first_indexed | 2024-04-10T05:43:15Z |
| format | Article |
| id | doaj.art-87390a17b5594d9bb542d659e4ddc4b7 |
| institution | Directory Open Access Journal |
| issn | 2713-4148 |
| language | English |
| last_indexed | 2024-04-10T05:43:15Z |
| publishDate | 2023-03-01 |
| publisher | The Korean Pediatric Society |
| record_format | Article |
| series | Clinical and Experimental Pediatrics |
| spelling | doaj.art-87390a17b5594d9bb542d659e4ddc4b72023-03-06T05:24:26ZengThe Korean Pediatric SocietyClinical and Experimental Pediatrics2713-41482023-03-0166312713310.3345/cep.2022.0092020125555599Changes and correlations of T-cell coinhibitory molecule programmed death-1 and interferon-γ in pediatric immune thrombocytopeniaFady Mohamed El-Gendy0Amira M.F. Shehata1Esam Awad Abd El-Kawy2Mahmoud Ahmed El-Hawy3 Pediatrics Department, Faculty of Medicine, Menoufia University, Shebin Al Kom, Egypt Clinical Pathology Department, Faculty of Medicine, Menoufia University, Shebin Al Kom, Egypt Pediatrics Department, Faculty of Medicine, Menoufia University, Shebin Al Kom, Egypt Pediatrics Department, Faculty of Medicine, Menoufia University, Shebin Al Kom, EgyptBackground Immune thrombocytopenia (ITP) is an acquired autoimmune disease characterized by abnormalities of T cells subsets. Programmed death-1 (PD-1) is a co-signaling inhibitory molecule in T cells that is involved in many autoimmune diseases. Purpose Here we aimed to measure changes in PD-1 expression and serum interferon-γ (IFN-γ) levels before and 1 month after treatment in pediatric patients with newly diagnosed ITP. Methods We measured PD-1+ CD4+ T cells percentages using flow cytometry and the serum IFN-γ levels by enzyme-linked immunosorbent assay in 40 pediatric patients with ITP and 20 healthy controls. Results Compared with healthy controls, the PD-1+ CD4+ T cells percentages and serum IFN-γ levels were significantly higher in ITP patients before and 1 month after therapy. A correlation study revealed that PD-1+ CD4+ T cells percentage was negatively associated with platelet count and positively associated with IFN-γ level in patients with ITP. Furthermore, serum IFN-γ levels were significantly decreased in patients after treatment, but no significant change was detected in the percentage of PD-1+ CD4+ T cells before or 1 month after therapy. Conclusion PD-1+ CD4+ T cells expression and IFN-γ levels were increased in patients with ITP. These preliminary data suggest a potential role of PD-1+ CD4+ T cells as mediators of ITP. We also found a correlation between PD-1+ CD4+ T cells and both platelet counts and IFN-γ levels. These findings suggest a potential role of PD-1+ CD4+ T cells and IFN-γ in the pathogenesis of ITP. Further studies investigating PD-1 expression in different T-cell subsets, serum IFN-γ concentrations, and antiplatelet antibodies levels over a longer duration after therapy initiation could delineate the precise role of PD-1 in ITP pathogenesis. Consequently, novel nontraditional therapeutic strategies for ITP patients may become available.http://www.e-cep.org/upload/pdf/cep-2022-00920.pdfblood plateletsinterferon gammaprogrammed cell death 1 receptorpurpura |
| spellingShingle | Fady Mohamed El-Gendy Amira M.F. Shehata Esam Awad Abd El-Kawy Mahmoud Ahmed El-Hawy Changes and correlations of T-cell coinhibitory molecule programmed death-1 and interferon-γ in pediatric immune thrombocytopenia Clinical and Experimental Pediatrics blood platelets interferon gamma programmed cell death 1 receptor purpura |
| title | Changes and correlations of T-cell coinhibitory molecule programmed death-1 and interferon-γ in pediatric immune thrombocytopenia |
| title_full | Changes and correlations of T-cell coinhibitory molecule programmed death-1 and interferon-γ in pediatric immune thrombocytopenia |
| title_fullStr | Changes and correlations of T-cell coinhibitory molecule programmed death-1 and interferon-γ in pediatric immune thrombocytopenia |
| title_full_unstemmed | Changes and correlations of T-cell coinhibitory molecule programmed death-1 and interferon-γ in pediatric immune thrombocytopenia |
| title_short | Changes and correlations of T-cell coinhibitory molecule programmed death-1 and interferon-γ in pediatric immune thrombocytopenia |
| title_sort | changes and correlations of t cell coinhibitory molecule programmed death 1 and interferon γ in pediatric immune thrombocytopenia |
| topic | blood platelets interferon gamma programmed cell death 1 receptor purpura |
| url | http://www.e-cep.org/upload/pdf/cep-2022-00920.pdf |
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