Biocontainment Techniques and Applications for Yeast Biotechnology
Biocontainment techniques for genetically modified yeasts (GMYs) are pivotal due to the importance of these organisms for biotechnological processes and also due to the design of new yeast strains by using synthetic biology tools and technologies. Due to the large genetic modifications that many yea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/4/341 |
| _version_ | 1797605516799639552 |
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| author | Guilherme Pavão Isabela Sfalcin Diego Bonatto |
| author_facet | Guilherme Pavão Isabela Sfalcin Diego Bonatto |
| author_sort | Guilherme Pavão |
| collection | DOAJ |
| description | Biocontainment techniques for genetically modified yeasts (GMYs) are pivotal due to the importance of these organisms for biotechnological processes and also due to the design of new yeast strains by using synthetic biology tools and technologies. Due to the large genetic modifications that many yeast strains display, it is highly desirable to avoid the leakage of GMY cells into natural environments and, consequently, the spread of synthetic genes and circuits by horizontal or vertical gene transfer mechanisms within the microorganisms. Moreover, it is also desirable to avoid patented yeast gene technologies spreading outside the production facility. In this review, the different biocontainment technologies currently available for GMYs were evaluated. Interestingly, uniplex-type biocontainment approaches (UTBAs), which rely on nutrient auxotrophies induced by gene mutation or deletion or the expression of the simple kill switches apparatus, are still the major biocontainment approaches in use with GMY. While bacteria such as <i>Escherichia coli</i> account for advanced biocontainment technologies based on synthetic biology and multiplex-type biocontainment approaches (MTBAs), GMYs are distant from this scenario due to many reasons. Thus, a comparison of different UTBAs and MTBAs applied for GMY and genetically engineered microorganisms (GEMs) was made, indicating the major advances of biocontainment techniques for GMYs. |
| first_indexed | 2024-03-11T05:02:12Z |
| format | Article |
| id | doaj.art-873c87a126b34928aa988f660b5e0d67 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T05:02:12Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-873c87a126b34928aa988f660b5e0d672023-11-17T19:10:31ZengMDPI AGFermentation2311-56372023-03-019434110.3390/fermentation9040341Biocontainment Techniques and Applications for Yeast BiotechnologyGuilherme Pavão0Isabela Sfalcin1Diego Bonatto2Laboratório de Biologia Molecular e Computacional, Centro de Biotecnologia da UFRGS, Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre 91509-900, RS, BrazilLaboratório de Biologia Molecular e Computacional, Centro de Biotecnologia da UFRGS, Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre 91509-900, RS, BrazilLaboratório de Biologia Molecular e Computacional, Centro de Biotecnologia da UFRGS, Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre 91509-900, RS, BrazilBiocontainment techniques for genetically modified yeasts (GMYs) are pivotal due to the importance of these organisms for biotechnological processes and also due to the design of new yeast strains by using synthetic biology tools and technologies. Due to the large genetic modifications that many yeast strains display, it is highly desirable to avoid the leakage of GMY cells into natural environments and, consequently, the spread of synthetic genes and circuits by horizontal or vertical gene transfer mechanisms within the microorganisms. Moreover, it is also desirable to avoid patented yeast gene technologies spreading outside the production facility. In this review, the different biocontainment technologies currently available for GMYs were evaluated. Interestingly, uniplex-type biocontainment approaches (UTBAs), which rely on nutrient auxotrophies induced by gene mutation or deletion or the expression of the simple kill switches apparatus, are still the major biocontainment approaches in use with GMY. While bacteria such as <i>Escherichia coli</i> account for advanced biocontainment technologies based on synthetic biology and multiplex-type biocontainment approaches (MTBAs), GMYs are distant from this scenario due to many reasons. Thus, a comparison of different UTBAs and MTBAs applied for GMY and genetically engineered microorganisms (GEMs) was made, indicating the major advances of biocontainment techniques for GMYs.https://www.mdpi.com/2311-5637/9/4/341biocontainmentgenetically engineered microorganismsgenetically modified yeastssynthetic biologygene circuitsauxotrophies |
| spellingShingle | Guilherme Pavão Isabela Sfalcin Diego Bonatto Biocontainment Techniques and Applications for Yeast Biotechnology Fermentation biocontainment genetically engineered microorganisms genetically modified yeasts synthetic biology gene circuits auxotrophies |
| title | Biocontainment Techniques and Applications for Yeast Biotechnology |
| title_full | Biocontainment Techniques and Applications for Yeast Biotechnology |
| title_fullStr | Biocontainment Techniques and Applications for Yeast Biotechnology |
| title_full_unstemmed | Biocontainment Techniques and Applications for Yeast Biotechnology |
| title_short | Biocontainment Techniques and Applications for Yeast Biotechnology |
| title_sort | biocontainment techniques and applications for yeast biotechnology |
| topic | biocontainment genetically engineered microorganisms genetically modified yeasts synthetic biology gene circuits auxotrophies |
| url | https://www.mdpi.com/2311-5637/9/4/341 |
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