Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology

Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology

Membrane proteins frequently assemble into higher order homo- or hetero-oligomers within their natural lipid environment. This complex formation can modulate their folding, activity as well as substrate selectivity. Non-disruptive methods avoiding critical steps, such as membrane disintegration, tra...

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Bibliographic Details
Main Authors: Erik Henrich, Oliver Peetz, Christopher Hein, Aisha Laguerre, Beate Hoffmann, Jan Hoffmann, Volker Dötsch, Frank Bernhard, Nina Morgner
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2017-01-01
Series:eLife
Subjects:
cell-free synthetic biology
non-covalent mass spectrometry
nanodisc
membrane protein
oligomeric state
Online Access:https://elifesciences.org/articles/20954

Internet

https://elifesciences.org/articles/20954

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