MALDI-TOF MS-Based KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-Term Cultures, and Colonies at the Hospital
Carbapenemase resistance in <i>Enterobacterales</i> is a global public health problem and rapid and effective methods for detecting these resistance mechanisms are needed urgently. Our aim was to evaluate the performance of a MALDI-TOF MS-based “<i>Klebsiella pneumoniae</i> c...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/7/865 |
| _version_ | 1797587873463009280 |
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| author | Agustina Costa Roque Figueroa-Espinosa Jerson A. Martínez Liliana Fernández-Canigia María I. Maldonado Silvina A. Bergese Ana E. Schneider Carlos Vay Carlos H. Rodriguez Marcela Nastro Gabriel O. Gutkind José A. Di Conza |
| author_facet | Agustina Costa Roque Figueroa-Espinosa Jerson A. Martínez Liliana Fernández-Canigia María I. Maldonado Silvina A. Bergese Ana E. Schneider Carlos Vay Carlos H. Rodriguez Marcela Nastro Gabriel O. Gutkind José A. Di Conza |
| author_sort | Agustina Costa |
| collection | DOAJ |
| description | Carbapenemase resistance in <i>Enterobacterales</i> is a global public health problem and rapid and effective methods for detecting these resistance mechanisms are needed urgently. Our aim was to evaluate the performance of a MALDI-TOF MS-based “<i>Klebsiella pneumoniae</i> carbapenemase” (KPC) detection protocol from patients’ positive blood cultures, short-term cultures, and colonies in healthcare settings. Bacterial identification and KPC detection were achieved after protein extraction with organic solvents and target spot loading with suitable organic matrices. The confirmation of KPC production was performed using susceptibility tests and <i>bla</i><sub>KPC</sub> amplification using PCR and sequencing. The KPC direct detection (KPC peak at approximately 28.681 Da) from patients’ positive blood cultures, short-term cultures, and colonies, once bacterial identification was achieved, showed an overall sensibility and specificity of 100% (CI95: [95%, 100%] and CI95: [99%, 100%], respectively). The concordance between hospital routine bacterial identification protocol and identification using this new methodology from the same extract used for KPC detection was ≥92%. This study represents the pioneering effort to directly detect KPC using MALDI-TOF MS technology, conducted on patient-derived samples obtained from hospitals for validation purposes, in a multi-resistance global context that requires concrete actions to preserve the available therapeutic options and reduce the spread of antibiotic resistance markers. |
| first_indexed | 2024-03-11T00:44:59Z |
| format | Article |
| id | doaj.art-873d7cfee2194d21b3ccd140b855612b |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T00:44:59Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-873d7cfee2194d21b3ccd140b855612b2023-11-18T20:50:05ZengMDPI AGPathogens2076-08172023-06-0112786510.3390/pathogens12070865MALDI-TOF MS-Based KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-Term Cultures, and Colonies at the HospitalAgustina Costa0Roque Figueroa-Espinosa1Jerson A. Martínez2Liliana Fernández-Canigia3María I. Maldonado4Silvina A. Bergese5Ana E. Schneider6Carlos Vay7Carlos H. Rodriguez8Marcela Nastro9Gabriel O. Gutkind10José A. Di Conza11Instituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires 1113, ArgentinaInstituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires 1113, ArgentinaInstituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires 1113, ArgentinaHospital Alemán de Buenos Aires, Buenos Aires 1113, ArgentinaHospital Alemán de Buenos Aires, Buenos Aires 1113, ArgentinaHospital Alemán de Buenos Aires, Buenos Aires 1113, ArgentinaHospital Alemán de Buenos Aires, Buenos Aires 1113, ArgentinaHospital de Clínicas “José de San Martín”, Universidad de Buenos Aires, Buenos Aires 1118, ArgentinaHospital de Clínicas “José de San Martín”, Universidad de Buenos Aires, Buenos Aires 1118, ArgentinaHospital de Clínicas “José de San Martín”, Universidad de Buenos Aires, Buenos Aires 1118, ArgentinaInstituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires 1113, ArgentinaInstituto de Investigaciones en Bacteriología y Virología Molecular (IBaViM), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires 1113, ArgentinaCarbapenemase resistance in <i>Enterobacterales</i> is a global public health problem and rapid and effective methods for detecting these resistance mechanisms are needed urgently. Our aim was to evaluate the performance of a MALDI-TOF MS-based “<i>Klebsiella pneumoniae</i> carbapenemase” (KPC) detection protocol from patients’ positive blood cultures, short-term cultures, and colonies in healthcare settings. Bacterial identification and KPC detection were achieved after protein extraction with organic solvents and target spot loading with suitable organic matrices. The confirmation of KPC production was performed using susceptibility tests and <i>bla</i><sub>KPC</sub> amplification using PCR and sequencing. The KPC direct detection (KPC peak at approximately 28.681 Da) from patients’ positive blood cultures, short-term cultures, and colonies, once bacterial identification was achieved, showed an overall sensibility and specificity of 100% (CI95: [95%, 100%] and CI95: [99%, 100%], respectively). The concordance between hospital routine bacterial identification protocol and identification using this new methodology from the same extract used for KPC detection was ≥92%. This study represents the pioneering effort to directly detect KPC using MALDI-TOF MS technology, conducted on patient-derived samples obtained from hospitals for validation purposes, in a multi-resistance global context that requires concrete actions to preserve the available therapeutic options and reduce the spread of antibiotic resistance markers.https://www.mdpi.com/2076-0817/12/7/865KPCblood cultureshort-term cultureMALDI-TOF MS |
| spellingShingle | Agustina Costa Roque Figueroa-Espinosa Jerson A. Martínez Liliana Fernández-Canigia María I. Maldonado Silvina A. Bergese Ana E. Schneider Carlos Vay Carlos H. Rodriguez Marcela Nastro Gabriel O. Gutkind José A. Di Conza MALDI-TOF MS-Based KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-Term Cultures, and Colonies at the Hospital Pathogens KPC blood culture short-term culture MALDI-TOF MS |
| title | MALDI-TOF MS-Based KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-Term Cultures, and Colonies at the Hospital |
| title_full | MALDI-TOF MS-Based KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-Term Cultures, and Colonies at the Hospital |
| title_fullStr | MALDI-TOF MS-Based KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-Term Cultures, and Colonies at the Hospital |
| title_full_unstemmed | MALDI-TOF MS-Based KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-Term Cultures, and Colonies at the Hospital |
| title_short | MALDI-TOF MS-Based KPC Direct Detection from Patients’ Positive Blood Culture Bottles, Short-Term Cultures, and Colonies at the Hospital |
| title_sort | maldi tof ms based kpc direct detection from patients positive blood culture bottles short term cultures and colonies at the hospital |
| topic | KPC blood culture short-term culture MALDI-TOF MS |
| url | https://www.mdpi.com/2076-0817/12/7/865 |
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