Presence of Different Candida Species at Denture Wearers With Type 2 Diabetes and Clinically Healthy Oral Mucosa-Pilot Study
Background/Aim: The aim of this study was to examine prevalence of different Candida spp. at diabetics and nondiabetics wearing dentures without clinical signs of Denture Stomatitis (DS) and to study if some local and systematic factors are confounders for harboring Candida at these subjects. Materi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Balkan Stomatological Society
2018-03-01
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| Series: | Balkan Journal of Dental Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/bjdm-2018-0003 |
| _version_ | 1828782581948088320 |
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| author | Petrović Sanja Matić Barać Milena Pfićer Jovana Kuzmanović Radunović Milena Jotić Aleksandra Pucar Ana |
| author_facet | Petrović Sanja Matić Barać Milena Pfićer Jovana Kuzmanović Radunović Milena Jotić Aleksandra Pucar Ana |
| author_sort | Petrović Sanja Matić |
| collection | DOAJ |
| description | Background/Aim: The aim of this study was to examine prevalence of different Candida spp. at diabetics and nondiabetics wearing dentures without clinical signs of Denture Stomatitis (DS) and to study if some local and systematic factors are confounders for harboring Candida at these subjects. Material and Methods: Total of 60 subjects wearing partial or complete upper acrylic denture having at least half of palatal mucosa covered by denture were selected and stratified into three experimental groups: systematically health subjects; patients with diagnosed Type 2 Diabetes (T2D) and good glycoregulation; and T2D subjects with poorly regulated blood sugar level. Cotton swab samples were obtained from each patient from hard palate mucosa and denture surface. Swab cultures were made on Sabouraud dextrose agar and ChromAgar Media for distinciton of various Candida spp. Density growth was also measured. Results: Frequency of Candida spp. findings were similar between groups. At healthy subjects, only C.albicans was detected. At diabetics, C.albicans was the most common isolated species, followed by C.glabrata and C.tropicalis. Negative finding of yeasts on palatal mucosa, but positive on denture surface were detected at all groups, with the highest frequency (33.4%) at diabetics with poor glycoregulation. Denture surface was heavier colonized than hard palate mucosa. Duration of diabetes in years were only independent predictors for harboring Candida spp. at denture surface (Exp B=1.186, CI=1.047-1.344, p=0.007). Conclusions: Prosthesis of denture wearers without DS may serve as reservoir of Candida spp. Presence of more pathogenic and resistant non-albicans species are related to diabetics, even without clinical signs of DS. |
| first_indexed | 2024-12-11T17:52:01Z |
| format | Article |
| id | doaj.art-8743b95b6a09464cace7926bcaad05e3 |
| institution | Directory Open Access Journal |
| issn | 2335-0245 |
| language | English |
| last_indexed | 2024-12-11T17:52:01Z |
| publishDate | 2018-03-01 |
| publisher | Balkan Stomatological Society |
| record_format | Article |
| series | Balkan Journal of Dental Medicine |
| spelling | doaj.art-8743b95b6a09464cace7926bcaad05e32022-12-22T00:56:11ZengBalkan Stomatological SocietyBalkan Journal of Dental Medicine2335-02452018-03-01221152110.2478/bjdm-2018-0003bjdm-2018-0003Presence of Different Candida Species at Denture Wearers With Type 2 Diabetes and Clinically Healthy Oral Mucosa-Pilot StudyPetrović Sanja Matić0Barać Milena1Pfićer Jovana Kuzmanović2Radunović Milena3Jotić Aleksandra4Pucar Ana5School of Dental Medicine, University of Belgrade, Belgrade, SerbiaSchool of Dental Medicine, University of Belgrade, Belgrade, SerbiaSchool of Dental Medicine, University of Belgrade, Belgrade, SerbiaSchool of Dental Medicine, University of Belgrade, Belgrade, SerbiaFaculty of Medicine University of Belgrade, Belgrade, SerbiaSchool of Dental Medicine, University of Belgrade, Belgrade, SerbiaBackground/Aim: The aim of this study was to examine prevalence of different Candida spp. at diabetics and nondiabetics wearing dentures without clinical signs of Denture Stomatitis (DS) and to study if some local and systematic factors are confounders for harboring Candida at these subjects. Material and Methods: Total of 60 subjects wearing partial or complete upper acrylic denture having at least half of palatal mucosa covered by denture were selected and stratified into three experimental groups: systematically health subjects; patients with diagnosed Type 2 Diabetes (T2D) and good glycoregulation; and T2D subjects with poorly regulated blood sugar level. Cotton swab samples were obtained from each patient from hard palate mucosa and denture surface. Swab cultures were made on Sabouraud dextrose agar and ChromAgar Media for distinciton of various Candida spp. Density growth was also measured. Results: Frequency of Candida spp. findings were similar between groups. At healthy subjects, only C.albicans was detected. At diabetics, C.albicans was the most common isolated species, followed by C.glabrata and C.tropicalis. Negative finding of yeasts on palatal mucosa, but positive on denture surface were detected at all groups, with the highest frequency (33.4%) at diabetics with poor glycoregulation. Denture surface was heavier colonized than hard palate mucosa. Duration of diabetes in years were only independent predictors for harboring Candida spp. at denture surface (Exp B=1.186, CI=1.047-1.344, p=0.007). Conclusions: Prosthesis of denture wearers without DS may serve as reservoir of Candida spp. Presence of more pathogenic and resistant non-albicans species are related to diabetics, even without clinical signs of DS.https://doi.org/10.2478/bjdm-2018-0003stomatitisdenturecandida albicansdiabetes mellitus type 2denturecompletedenturepartialremovable |
| spellingShingle | Petrović Sanja Matić Barać Milena Pfićer Jovana Kuzmanović Radunović Milena Jotić Aleksandra Pucar Ana Presence of Different Candida Species at Denture Wearers With Type 2 Diabetes and Clinically Healthy Oral Mucosa-Pilot Study Balkan Journal of Dental Medicine stomatitis denture candida albicans diabetes mellitus type 2 denture complete denture partial removable |
| title | Presence of Different Candida Species at Denture Wearers With Type 2 Diabetes and Clinically Healthy Oral Mucosa-Pilot Study |
| title_full | Presence of Different Candida Species at Denture Wearers With Type 2 Diabetes and Clinically Healthy Oral Mucosa-Pilot Study |
| title_fullStr | Presence of Different Candida Species at Denture Wearers With Type 2 Diabetes and Clinically Healthy Oral Mucosa-Pilot Study |
| title_full_unstemmed | Presence of Different Candida Species at Denture Wearers With Type 2 Diabetes and Clinically Healthy Oral Mucosa-Pilot Study |
| title_short | Presence of Different Candida Species at Denture Wearers With Type 2 Diabetes and Clinically Healthy Oral Mucosa-Pilot Study |
| title_sort | presence of different candida species at denture wearers with type 2 diabetes and clinically healthy oral mucosa pilot study |
| topic | stomatitis denture candida albicans diabetes mellitus type 2 denture complete denture partial removable |
| url | https://doi.org/10.2478/bjdm-2018-0003 |
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