Hydrolytic degradation of methoxychlor by immobilized cellulase on LDHs@Fe3O4 nanocomposites
In this study, we synthesized Fe3O4 using the co-precipitation method and then prepared magnetic carrier LDHs@Fe3O4 by immobilizing layered double hydroxide on Fe3O4 by in situ growth method. Cellulase was immobilized on this magnetic carrier by using glutaraldehyde as a coupling agent, which can be...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
IWA Publishing
2024-01-01
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| Series: | Water Science and Technology |
| Subjects: | |
| Online Access: | http://wst.iwaponline.com/content/89/1/170 |
| Summary: | In this study, we synthesized Fe3O4 using the co-precipitation method and then prepared magnetic carrier LDHs@Fe3O4 by immobilizing layered double hydroxide on Fe3O4 by in situ growth method. Cellulase was immobilized on this magnetic carrier by using glutaraldehyde as a coupling agent, which can be used for degrading Methoxychlor (MXC). The results demonstrated the maximum MXC removal efficiency of 73.4% at 45 °C and pH = 6.0 with excellent reusability. Through kinetic analysis, it was found that the degradation reaction conforms to the Langmuir–Hinshelwood model and is a first-order reaction. Finally, according to the EPR analysis, the active radicals in the system were found to be OH· and the degradation mechanism was proposed in combination with LC-MS. This study provides a feasible method for degrading organochlorine pesticides, which can be used for groundwater purification.
HIGHLIGHTS
Cellulase immobilized on magnetic LDHs carrier by covalent binding route.;
The optimum temperature for the immobilized cellulase was 45 °C, indicating that magnetic LDHs can provide thermal acceleration of the degradation of the underlying cyclic oligomers.;
The magnetic LDHs carrier can decrease the activation energy for MXC degradation reaction significantly.; |
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| ISSN: | 0273-1223 1996-9732 |