Surface Dependent Inhibition of Mycobacterium abscessus by Diverse Pseudomonas aeruginosa Strains
ABSTRACT Both Pseudomonas aeruginosa and Mycobacterium abscessus are bacteria that cause pulmonary infection in people with inflammatory lung disease, including individuals with cystic fibrosis (CF). These bacterial species inhabit the same environmental reservoirs (soil and water) and can be coisol...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2022-12-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02471-22 |
| _version_ | 1798003837099835392 |
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| author | Ayantu W. Idosa Daniel J. Wozniak Luanne Hall-Stoodley |
| author_facet | Ayantu W. Idosa Daniel J. Wozniak Luanne Hall-Stoodley |
| author_sort | Ayantu W. Idosa |
| collection | DOAJ |
| description | ABSTRACT Both Pseudomonas aeruginosa and Mycobacterium abscessus are bacteria that cause pulmonary infection in people with inflammatory lung disease, including individuals with cystic fibrosis (CF). These bacterial species inhabit the same environmental reservoirs (soil and water) and can be coisolated in the lungs of people with CF. We investigated the interaction of these bacteria and found an antagonistic interaction favoring P. aeruginosa that was observed in biofilms but not in planktonic cultures. This antagonism extended to multiple P. aeruginosa strains and against Mycobacterium smegmatis. We tested known P. aeruginosa mutants for genes that can play roles in interbacterial contact-dependent (type III and type VI secretion systems) and contact-independent (quorum sensing, type II secretion) antagonism pathways to interrogate the mechanism of action. Our results indicate that well-known mechanisms of interbacterial competition are not responsible for the antagonism of P. aeruginosa toward M. abscessus, suggesting a novel antibacterial strategy. IMPORTANCE The biofilm lifestyle is favored by many organisms, and understanding interbacterial interactions that occur between coisolated bacterial species can provide new information regarding bacterial defense mechanisms and antibacterial targets. This may also provide insights into possible interbacterial interactions impacting host immunity during coinfection. Here, we investigate an antagonistic interaction favoring P. aeruginosa over M. abscessus exclusively in dual-species biofilms and not in liquid coculture. |
| first_indexed | 2024-04-11T12:14:00Z |
| format | Article |
| id | doaj.art-874bd1a63f1b45ecad1b6a208b0594d6 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-11T12:14:00Z |
| publishDate | 2022-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-874bd1a63f1b45ecad1b6a208b0594d62022-12-22T04:24:25ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972022-12-0110610.1128/spectrum.02471-22Surface Dependent Inhibition of Mycobacterium abscessus by Diverse Pseudomonas aeruginosa StrainsAyantu W. Idosa0Daniel J. Wozniak1Luanne Hall-Stoodley2Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USADepartment of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USADepartment of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USAABSTRACT Both Pseudomonas aeruginosa and Mycobacterium abscessus are bacteria that cause pulmonary infection in people with inflammatory lung disease, including individuals with cystic fibrosis (CF). These bacterial species inhabit the same environmental reservoirs (soil and water) and can be coisolated in the lungs of people with CF. We investigated the interaction of these bacteria and found an antagonistic interaction favoring P. aeruginosa that was observed in biofilms but not in planktonic cultures. This antagonism extended to multiple P. aeruginosa strains and against Mycobacterium smegmatis. We tested known P. aeruginosa mutants for genes that can play roles in interbacterial contact-dependent (type III and type VI secretion systems) and contact-independent (quorum sensing, type II secretion) antagonism pathways to interrogate the mechanism of action. Our results indicate that well-known mechanisms of interbacterial competition are not responsible for the antagonism of P. aeruginosa toward M. abscessus, suggesting a novel antibacterial strategy. IMPORTANCE The biofilm lifestyle is favored by many organisms, and understanding interbacterial interactions that occur between coisolated bacterial species can provide new information regarding bacterial defense mechanisms and antibacterial targets. This may also provide insights into possible interbacterial interactions impacting host immunity during coinfection. Here, we investigate an antagonistic interaction favoring P. aeruginosa over M. abscessus exclusively in dual-species biofilms and not in liquid coculture.https://journals.asm.org/doi/10.1128/spectrum.02471-22Pseudomonas aeruginosaMycobacterium abscessusinterbacterial interactionantagonismbiofilmsbacteria-bacteria interactions |
| spellingShingle | Ayantu W. Idosa Daniel J. Wozniak Luanne Hall-Stoodley Surface Dependent Inhibition of Mycobacterium abscessus by Diverse Pseudomonas aeruginosa Strains Microbiology Spectrum Pseudomonas aeruginosa Mycobacterium abscessus interbacterial interaction antagonism biofilms bacteria-bacteria interactions |
| title | Surface Dependent Inhibition of Mycobacterium abscessus by Diverse Pseudomonas aeruginosa Strains |
| title_full | Surface Dependent Inhibition of Mycobacterium abscessus by Diverse Pseudomonas aeruginosa Strains |
| title_fullStr | Surface Dependent Inhibition of Mycobacterium abscessus by Diverse Pseudomonas aeruginosa Strains |
| title_full_unstemmed | Surface Dependent Inhibition of Mycobacterium abscessus by Diverse Pseudomonas aeruginosa Strains |
| title_short | Surface Dependent Inhibition of Mycobacterium abscessus by Diverse Pseudomonas aeruginosa Strains |
| title_sort | surface dependent inhibition of mycobacterium abscessus by diverse pseudomonas aeruginosa strains |
| topic | Pseudomonas aeruginosa Mycobacterium abscessus interbacterial interaction antagonism biofilms bacteria-bacteria interactions |
| url | https://journals.asm.org/doi/10.1128/spectrum.02471-22 |
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