Development of a Culture Medium for Microalgae Production Based on Minimal Processing of Oil Palm Biomass Ash
With the increasing participation of biomass in the world energy matrix, large amounts of ash are produced through combustion, resulting in the need to dispose of this waste to minimize the environmental impact. An alternative is to use ashes as phosphorus supplements in microalgae cultures. The pre...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/2/55 |
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| author | Lorenzo Ferrari Assú Tessari Carlos Ricardo Soccol Cristine Rodrigues Estefania García González Valcineide Oliveira de Andrade Tanobe Paulo Cesar de Souza Kirnev Júlio Cesar de Carvalho |
| author_facet | Lorenzo Ferrari Assú Tessari Carlos Ricardo Soccol Cristine Rodrigues Estefania García González Valcineide Oliveira de Andrade Tanobe Paulo Cesar de Souza Kirnev Júlio Cesar de Carvalho |
| author_sort | Lorenzo Ferrari Assú Tessari |
| collection | DOAJ |
| description | With the increasing participation of biomass in the world energy matrix, large amounts of ash are produced through combustion, resulting in the need to dispose of this waste to minimize the environmental impact. An alternative is to use ashes as phosphorus supplements in microalgae cultures. The present work describes the development and use of a balanced culture medium based on the minimal processing of oil palm biomass ash to cultivate <i>Arthrospira platensis</i> Paracas, <i>Neochloris oleoabundans</i> UTEX 1185, and <i>Dunaliella salina</i> SAG 184. The acid extraction process of phosphorus (P) was defined by evaluating the following parameters: temperature (20 to 70 °C), acid load (0.01 to 0.03 mols/g of ash) of HNO<sub>3</sub>, and liquid/solid ratio (50 to 150 mLg<sup>−1</sup>). The best efficiency of the extraction process was 97%. The use of HNO<sub>3</sub> allowed for the production of an extract containing balanced amounts of N and P sources, the BAX medium (Biomass Ash Extract). This medium was efficient for cultivating the three microorganisms studied, reaching biomass concentrations of 2.03, 0.902, and 0.69 g/L or 84%, 82%, and 99% of the control concentrations for <i>A. platensis</i>, <i>N. Oleoabundans,</i> and <i>D. salina</i>, respectively. In a final scaling-up test, <i>A. platensis</i> showed productivity of 0.047 g L<sup>−1</sup>d<sup>−1</sup> in a 120 L tank in a greenhouse. BAX can be an alternative nutrient medium for microalgae cultivation, especially in integration with biomass-fueled biorefineries. |
| first_indexed | 2024-03-09T22:00:43Z |
| format | Article |
| id | doaj.art-874f7a05cf144bd7898244bacd5b3ace |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T22:00:43Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-874f7a05cf144bd7898244bacd5b3ace2023-11-23T19:49:56ZengMDPI AGFermentation2311-56372022-01-01825510.3390/fermentation8020055Development of a Culture Medium for Microalgae Production Based on Minimal Processing of Oil Palm Biomass AshLorenzo Ferrari Assú Tessari0Carlos Ricardo Soccol1Cristine Rodrigues2Estefania García González3Valcineide Oliveira de Andrade Tanobe4Paulo Cesar de Souza Kirnev5Júlio Cesar de Carvalho6Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Brazil, Centro Politécnico, CP 19011, Curitiba 81531-980, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Brazil, Centro Politécnico, CP 19011, Curitiba 81531-980, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Brazil, Centro Politécnico, CP 19011, Curitiba 81531-980, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Brazil, Centro Politécnico, CP 19011, Curitiba 81531-980, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Brazil, Centro Politécnico, CP 19011, Curitiba 81531-980, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Brazil, Centro Politécnico, CP 19011, Curitiba 81531-980, PR, BrazilDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Brazil, Centro Politécnico, CP 19011, Curitiba 81531-980, PR, BrazilWith the increasing participation of biomass in the world energy matrix, large amounts of ash are produced through combustion, resulting in the need to dispose of this waste to minimize the environmental impact. An alternative is to use ashes as phosphorus supplements in microalgae cultures. The present work describes the development and use of a balanced culture medium based on the minimal processing of oil palm biomass ash to cultivate <i>Arthrospira platensis</i> Paracas, <i>Neochloris oleoabundans</i> UTEX 1185, and <i>Dunaliella salina</i> SAG 184. The acid extraction process of phosphorus (P) was defined by evaluating the following parameters: temperature (20 to 70 °C), acid load (0.01 to 0.03 mols/g of ash) of HNO<sub>3</sub>, and liquid/solid ratio (50 to 150 mLg<sup>−1</sup>). The best efficiency of the extraction process was 97%. The use of HNO<sub>3</sub> allowed for the production of an extract containing balanced amounts of N and P sources, the BAX medium (Biomass Ash Extract). This medium was efficient for cultivating the three microorganisms studied, reaching biomass concentrations of 2.03, 0.902, and 0.69 g/L or 84%, 82%, and 99% of the control concentrations for <i>A. platensis</i>, <i>N. Oleoabundans,</i> and <i>D. salina</i>, respectively. In a final scaling-up test, <i>A. platensis</i> showed productivity of 0.047 g L<sup>−1</sup>d<sup>−1</sup> in a 120 L tank in a greenhouse. BAX can be an alternative nutrient medium for microalgae cultivation, especially in integration with biomass-fueled biorefineries.https://www.mdpi.com/2311-5637/8/2/55phosphorusmicroalgae<i>Dunaliella</i><i>Arthrospira</i><i>Neochloris</i>nitric acid |
| spellingShingle | Lorenzo Ferrari Assú Tessari Carlos Ricardo Soccol Cristine Rodrigues Estefania García González Valcineide Oliveira de Andrade Tanobe Paulo Cesar de Souza Kirnev Júlio Cesar de Carvalho Development of a Culture Medium for Microalgae Production Based on Minimal Processing of Oil Palm Biomass Ash Fermentation phosphorus microalgae <i>Dunaliella</i> <i>Arthrospira</i> <i>Neochloris</i> nitric acid |
| title | Development of a Culture Medium for Microalgae Production Based on Minimal Processing of Oil Palm Biomass Ash |
| title_full | Development of a Culture Medium for Microalgae Production Based on Minimal Processing of Oil Palm Biomass Ash |
| title_fullStr | Development of a Culture Medium for Microalgae Production Based on Minimal Processing of Oil Palm Biomass Ash |
| title_full_unstemmed | Development of a Culture Medium for Microalgae Production Based on Minimal Processing of Oil Palm Biomass Ash |
| title_short | Development of a Culture Medium for Microalgae Production Based on Minimal Processing of Oil Palm Biomass Ash |
| title_sort | development of a culture medium for microalgae production based on minimal processing of oil palm biomass ash |
| topic | phosphorus microalgae <i>Dunaliella</i> <i>Arthrospira</i> <i>Neochloris</i> nitric acid |
| url | https://www.mdpi.com/2311-5637/8/2/55 |
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