Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestris
Objective: To isolate endophytic fungi from Elaeocarpus sylvestris (E. sylvestris) and to isolate antioxidant compounds from a potential source fungus. Methods: Endophytic fungi were isolated from fresh leaves and stems of E. sylvestris and identified based on DNA analysis. 1,1-Diphenyl-2-picrylhydr...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-02-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169116304750 |
| _version_ | 1818822125557907456 |
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| author | Amalia Indah Prihantini Sanro Tachibana |
| author_facet | Amalia Indah Prihantini Sanro Tachibana |
| author_sort | Amalia Indah Prihantini |
| collection | DOAJ |
| description | Objective: To isolate endophytic fungi from Elaeocarpus sylvestris (E. sylvestris) and to isolate antioxidant compounds from a potential source fungus.
Methods: Endophytic fungi were isolated from fresh leaves and stems of E. sylvestris and identified based on DNA analysis. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was used to evaluate the antioxidant activity of the fungi. The potential antioxidant fungus was further studied to isolate antioxidant compounds. The isolated compounds were identified by melting point analysis, optical rotation, spectral analysis using a UV spectrophotometer, high resolution fast atom bombardment mass spectrometry, X-ray crystallography analysis, 1H nuclear magnetic resonance analysis and 13C nuclear magnetic resonance analysis. The isolated compounds were evaluated with DPPH radical scavenging, reducing power, and β-carotene bleaching assays.
Results: Seven endophytic fungi were successfully isolated from E. sylvestris and identified as Pestalotiopsis sp. EST 01, Pestalotiopsis sp. EST 02, Diaporthales sp. EST 03, Meyerozyma sp. EST 04, Diaporthales sp. EST 05, Pestalotiopsis sp. ESL 01, and Pseudocercospora sp. ESL 02. Of the seven fungi, Pseudocercospora sp. ESL 02 had the highest antioxidant activity [IC50 = (30.54 ± 0.88) μg/mL]. From that fungus, two compounds identified as terreic acid (1) and 6-methylsalicylic acid (2) were isolated with an IC50 of DPPH radical scavenging activity of (0.22 ± 0.02) mmol/L and (3.87 ± 0.27) mmol/L, respectively. The compounds also had good activities from the reducing power and β-carotene bleaching assays.
Conclusions: The Pseudocercospora sp. ESL 02 fungus isolated from E. sylvestris looks promising as a novel source of terreic acid. |
| first_indexed | 2024-12-18T23:19:07Z |
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| id | doaj.art-87518922d40c4a1d8c4d64d7800ed6cf |
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| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-18T23:19:07Z |
| publishDate | 2017-02-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-87518922d40c4a1d8c4d64d7800ed6cf2022-12-21T20:48:03ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912017-02-017211011510.1016/j.apjtb.2016.11.020Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestrisAmalia Indah Prihantini0Sanro Tachibana1The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, JapanDepartment of Applied Bioscience, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, JapanObjective: To isolate endophytic fungi from Elaeocarpus sylvestris (E. sylvestris) and to isolate antioxidant compounds from a potential source fungus. Methods: Endophytic fungi were isolated from fresh leaves and stems of E. sylvestris and identified based on DNA analysis. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was used to evaluate the antioxidant activity of the fungi. The potential antioxidant fungus was further studied to isolate antioxidant compounds. The isolated compounds were identified by melting point analysis, optical rotation, spectral analysis using a UV spectrophotometer, high resolution fast atom bombardment mass spectrometry, X-ray crystallography analysis, 1H nuclear magnetic resonance analysis and 13C nuclear magnetic resonance analysis. The isolated compounds were evaluated with DPPH radical scavenging, reducing power, and β-carotene bleaching assays. Results: Seven endophytic fungi were successfully isolated from E. sylvestris and identified as Pestalotiopsis sp. EST 01, Pestalotiopsis sp. EST 02, Diaporthales sp. EST 03, Meyerozyma sp. EST 04, Diaporthales sp. EST 05, Pestalotiopsis sp. ESL 01, and Pseudocercospora sp. ESL 02. Of the seven fungi, Pseudocercospora sp. ESL 02 had the highest antioxidant activity [IC50 = (30.54 ± 0.88) μg/mL]. From that fungus, two compounds identified as terreic acid (1) and 6-methylsalicylic acid (2) were isolated with an IC50 of DPPH radical scavenging activity of (0.22 ± 0.02) mmol/L and (3.87 ± 0.27) mmol/L, respectively. The compounds also had good activities from the reducing power and β-carotene bleaching assays. Conclusions: The Pseudocercospora sp. ESL 02 fungus isolated from E. sylvestris looks promising as a novel source of terreic acid.http://www.sciencedirect.com/science/article/pii/S2221169116304750Elaeocarpus sylvestrisEndophytic fungiPseudocercospora sp.AntioxidantTerreic acid |
| spellingShingle | Amalia Indah Prihantini Sanro Tachibana Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestris Asian Pacific Journal of Tropical Biomedicine Elaeocarpus sylvestris Endophytic fungi Pseudocercospora sp. Antioxidant Terreic acid |
| title | Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestris |
| title_full | Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestris |
| title_fullStr | Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestris |
| title_full_unstemmed | Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestris |
| title_short | Antioxidant compounds produced by Pseudocercospora sp. ESL 02, an endophytic fungus isolated from Elaeocarpus sylvestris |
| title_sort | antioxidant compounds produced by pseudocercospora sp esl 02 an endophytic fungus isolated from elaeocarpus sylvestris |
| topic | Elaeocarpus sylvestris Endophytic fungi Pseudocercospora sp. Antioxidant Terreic acid |
| url | http://www.sciencedirect.com/science/article/pii/S2221169116304750 |
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