Cloning and Expression Analysis of a Giant Gourami Vasa-Like cDNA
Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in thi...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2011-09-01
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| Series: | Hayati Journal of Biosciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916302005 |
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| author | ALIMUDDIN IRMA ANDRIYANI MUHAMMAD ZAIRIN JUNIOR HARTON ARFAH ANNA OCTAVERA ODANG CARMAN GORO YOSHIZAKI |
| author_facet | ALIMUDDIN IRMA ANDRIYANI MUHAMMAD ZAIRIN JUNIOR HARTON ARFAH ANNA OCTAVERA ODANG CARMAN GORO YOSHIZAKI |
| author_sort | ALIMUDDIN |
| collection | DOAJ |
| description | Molecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells. |
| first_indexed | 2024-12-14T15:30:47Z |
| format | Article |
| id | doaj.art-875669738d814347bbeec0b1af865fa5 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-12-14T15:30:47Z |
| publishDate | 2011-09-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-875669738d814347bbeec0b1af865fa52022-12-21T22:55:53ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192011-09-0118313514010.4308/hjb.18.3.135Cloning and Expression Analysis of a Giant Gourami Vasa-Like cDNAALIMUDDIN0IRMA ANDRIYANI1MUHAMMAD ZAIRIN JUNIOR2HARTON ARFAH3ANNA OCTAVERA4ODANG CARMAN5GORO YOSHIZAKI6Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor 16680, IndonesiaDepartment of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor 16680, IndonesiaDepartment of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo 108-8477, JapanMolecular marker is useful in the development of testicular cells transplantation for detecting donor-derived germ cells in the recipient gonad. In this study, a giant gourami (Osphronemus goramy) vasa-like gene (GgVLG) was cloned and characterized for use as a molecular marker for germ cells in this species. Nucleotide sequence analysis revealed that GgVLG comprises 2,340 bps with an open reading frame of 1,962 bps encoding 653 amino acids. The deduced amino acid sequence contained 17 arginine-glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family. The GgVLG sequence showed high similarity to Drosophila vasa, common carp vasa homolog and tilapia vasa homolog for 66.2, 85.9, and 90.7%, respectively. In adult tissues, the GgVLG transcripts were specifically detected in ovary and testis. In situ hybridization analysis showed that GgVLG mRNA was detected in oocytes of the ovary and spermatogonia of the testis. There was no signal detected in the spermatocytes, spermatids and other gonadal somatic cells. Thus, consensus sequences, specific localization of GgVLG mRNA in the germ cells, amino acid sequence similarity and phylogenic analysis all suggest that GgVLG is the giant gourami vasa-like gene. Further, GgVLG can be used as a molecular marker for giant gourami germ cells.http://www.sciencedirect.com/science/article/pii/S1978301916302005germ cell transplantationovaryspermatogoniatestisgiant gouramivasa |
| spellingShingle | ALIMUDDIN IRMA ANDRIYANI MUHAMMAD ZAIRIN JUNIOR HARTON ARFAH ANNA OCTAVERA ODANG CARMAN GORO YOSHIZAKI Cloning and Expression Analysis of a Giant Gourami Vasa-Like cDNA Hayati Journal of Biosciences germ cell transplantation ovary spermatogonia testis giant gourami vasa |
| title | Cloning and Expression Analysis of a Giant Gourami Vasa-Like cDNA |
| title_full | Cloning and Expression Analysis of a Giant Gourami Vasa-Like cDNA |
| title_fullStr | Cloning and Expression Analysis of a Giant Gourami Vasa-Like cDNA |
| title_full_unstemmed | Cloning and Expression Analysis of a Giant Gourami Vasa-Like cDNA |
| title_short | Cloning and Expression Analysis of a Giant Gourami Vasa-Like cDNA |
| title_sort | cloning and expression analysis of a giant gourami vasa like cdna |
| topic | germ cell transplantation ovary spermatogonia testis giant gourami vasa |
| url | http://www.sciencedirect.com/science/article/pii/S1978301916302005 |
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