PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis
This study aimed to screen different winter wheat genotypes at the onset of metabolic changes induced by water deficit to comprehend possible adaptive features of photosynthetic apparatus function and structure to physiological drought. The drought treatment was the most influential variable affecti...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.987702/full |
| _version_ | 1811239282991431680 |
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| author | Vesna Peršić Anita Ament Jasenka Antunović Dunić Georg Drezner Vera Cesar Vera Cesar |
| author_facet | Vesna Peršić Anita Ament Jasenka Antunović Dunić Georg Drezner Vera Cesar Vera Cesar |
| author_sort | Vesna Peršić |
| collection | DOAJ |
| description | This study aimed to screen different winter wheat genotypes at the onset of metabolic changes induced by water deficit to comprehend possible adaptive features of photosynthetic apparatus function and structure to physiological drought. The drought treatment was the most influential variable affecting plant growth and relative water content, and genotype variability determined with what intensity varieties of winter wheat seedlings responded to water deficit. PEG-induced drought, as expected, changed phenomenological energy fluxes and the efficiency with which an electron is transferred to final PSI acceptors. Based on the effect size, fluorescence parameters were grouped to represent photochemical parameters, that is, the donor and acceptor side of PSII (PC1); the thermal phase of the photosynthetic process, or the electron flow around PSI, and the chain of electrons between PSII and PSI (PC2); and phenomenological energy fluxes per cross-section (PC3). Furthermore, four distinct clusters of genotypes were discerned based on their response to imposed physiological drought, and integrated analysis enabled an explanation of their reactions’ specificity. The most reliable JIP-test parameters for detecting and comparing the drought impact among tested genotypes were the variable fluorescence at K, L, I step, and PITOT. To conclude, developing and improving screening methods for identifying and evaluating functional relationships of relevant characteristics that are useful for acclimation, acclimatization, and adaptation to different types of drought stress can contribute to the progress in breeding research of winter wheat drought-tolerant lines. |
| first_indexed | 2024-04-12T12:56:58Z |
| format | Article |
| id | doaj.art-8757c5cda09d4778a3ffba196a97e116 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T12:56:58Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8757c5cda09d4778a3ffba196a97e1162022-12-22T03:32:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.987702987702PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysisVesna Peršić0Anita Ament1Jasenka Antunović Dunić2Georg Drezner3Vera Cesar4Vera Cesar5Department of Biology, Josip Juraj Strossmayer University of Osijek, Osijek, CroatiaDepartment of Biology, Josip Juraj Strossmayer University of Osijek, Osijek, CroatiaDepartment of Biology, Josip Juraj Strossmayer University of Osijek, Osijek, CroatiaDepartment of Small Cereal Crops, Agricultural Institute Osijek, Osijek, CroatiaDepartment of Biology, Josip Juraj Strossmayer University of Osijek, Osijek, CroatiaFaculty of Dental Medicine and Health, Josip Juraj Strossmayer University of Osijek, Osijek, CroatiaThis study aimed to screen different winter wheat genotypes at the onset of metabolic changes induced by water deficit to comprehend possible adaptive features of photosynthetic apparatus function and structure to physiological drought. The drought treatment was the most influential variable affecting plant growth and relative water content, and genotype variability determined with what intensity varieties of winter wheat seedlings responded to water deficit. PEG-induced drought, as expected, changed phenomenological energy fluxes and the efficiency with which an electron is transferred to final PSI acceptors. Based on the effect size, fluorescence parameters were grouped to represent photochemical parameters, that is, the donor and acceptor side of PSII (PC1); the thermal phase of the photosynthetic process, or the electron flow around PSI, and the chain of electrons between PSII and PSI (PC2); and phenomenological energy fluxes per cross-section (PC3). Furthermore, four distinct clusters of genotypes were discerned based on their response to imposed physiological drought, and integrated analysis enabled an explanation of their reactions’ specificity. The most reliable JIP-test parameters for detecting and comparing the drought impact among tested genotypes were the variable fluorescence at K, L, I step, and PITOT. To conclude, developing and improving screening methods for identifying and evaluating functional relationships of relevant characteristics that are useful for acclimation, acclimatization, and adaptation to different types of drought stress can contribute to the progress in breeding research of winter wheat drought-tolerant lines.https://www.frontiersin.org/articles/10.3389/fpls.2022.987702/fulltriticum aestivumPEG-6000photosynthesisfree prolinelipid peroxidationHAC (hierarchical agglomerative clustering) |
| spellingShingle | Vesna Peršić Anita Ament Jasenka Antunović Dunić Georg Drezner Vera Cesar Vera Cesar PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis Frontiers in Plant Science triticum aestivum PEG-6000 photosynthesis free proline lipid peroxidation HAC (hierarchical agglomerative clustering) |
| title | PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis |
| title_full | PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis |
| title_fullStr | PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis |
| title_full_unstemmed | PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis |
| title_short | PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis |
| title_sort | peg induced physiological drought for screening winter wheat genotypes sensitivity integrated biochemical and chlorophyll a fluorescence analysis |
| topic | triticum aestivum PEG-6000 photosynthesis free proline lipid peroxidation HAC (hierarchical agglomerative clustering) |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.987702/full |
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