CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through miR‐31‐5p/COL5A1 axis
Abstract Introduction Laryngeal squamous cell cancer (LSCC) is a highly malignant tumor originating from the respiratory system. Circular RNAs have been reported to be associated with the treatment and prognosis of a variety of cancers, including LSCC. Methods The expression of circBFAR, miR‐31‐5p,...
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| Format: | Article |
| Language: | English |
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Wiley
2022-12-01
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| Series: | Laryngoscope Investigative Otolaryngology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/lio2.966 |
| _version_ | 1811196204261834752 |
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| author | Hengcui Gong Wei Wu Chuankai Fang Di He |
| author_facet | Hengcui Gong Wei Wu Chuankai Fang Di He |
| author_sort | Hengcui Gong |
| collection | DOAJ |
| description | Abstract Introduction Laryngeal squamous cell cancer (LSCC) is a highly malignant tumor originating from the respiratory system. Circular RNAs have been reported to be associated with the treatment and prognosis of a variety of cancers, including LSCC. Methods The expression of circBFAR, miR‐31‐5p, and collagen type V alpha 1 chain (COL5A1) in LSCC tissues and cells was detected by quantitative real‐time polymerase chain reaction. Cell counting kit 8 and 5‐Ethynyl‐2′‐deoxyuridine assays were used to detect cell proliferation. Wound healing assay and transwell assay were used to test cell migration and invasion, respectively. The protein expression in LSCC cells was detected with western blot. The relationships between miR‐31‐5p and circBFAR or COL5A1 were identified by dual‐luciferase reporter assay, RNA‐pull down assay, and immunoprecipitation assay. The effect of circBFAR on tumor growth in vivo was detected by tumor xenograft mice experiment. The protein expression of COL5A1 and KI‐67 in LSCC tissues was measured by immunohistochemistry assay. Results CircBFAR was increased in LSCC tissues and cells, and was related to advanced clinical stage and overall survival of LSCC patients. The cell viability and proliferation were inhibited by circBFAR knockdown and silencing of circBFAR blocked migration and invasion of LSCC cells. CircBFAR knockdown suppressed cell tube formation, and the protein expression of KI‐67, matrix metallopeptidase 2 (MMP2), and vascular endothelial growth factor A (VEGFA) in LSCC cells. MiR‐31‐5p was the target of circBFAR, and the inhibitory effects of circBFAR deficiency on viability, proliferation, migration, invasion, tube formation and the protein expression of KI‐67, MMP2, and VEGFA in LSCC cells were rescued by miR‐31‐5p downregulation. COL5A1 was negatively regulated by miR‐31‐5p, and was boosted in LSCC tissues and cells. COL5A1 overexpression reversed the inhibitory effects of miR‐31‐5p on LSCC cells. CircBFAR insufficiency hindered tumor growth in vivo. Conclusion CircBFAR, miR‐31‐5p, and COL5A1 in LSCC progression might provide novel therapeutic targets for LSCC clinical intervention. |
| first_indexed | 2024-04-12T00:54:45Z |
| format | Article |
| id | doaj.art-87588b08c6e949309f393364836c058b |
| institution | Directory Open Access Journal |
| issn | 2378-8038 |
| language | English |
| last_indexed | 2024-04-12T00:54:45Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj.art-87588b08c6e949309f393364836c058b2022-12-22T03:54:38ZengWileyLaryngoscope Investigative Otolaryngology2378-80382022-12-01761951196210.1002/lio2.966CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through miR‐31‐5p/COL5A1 axisHengcui Gong0Wei Wu1Chuankai Fang2Di He3Department of Otolaryngology Yibin Hospital of Traditional Chinese Medicine Yibin Sichuan ChinaDepartment of Radiotherapy GanZhou Cancer Hospital/The Affiliated Cancer Hospital of Gannan Medical University GanZhou Jiangxi ChinaDepartment of Ophthalmology Tongxiang First people's Hospital Tongxiang Zhejiang ChinaDepartment of Otorhinolaryngology Tongxiang First people's Hospital Tongxiang Zhejiang ChinaAbstract Introduction Laryngeal squamous cell cancer (LSCC) is a highly malignant tumor originating from the respiratory system. Circular RNAs have been reported to be associated with the treatment and prognosis of a variety of cancers, including LSCC. Methods The expression of circBFAR, miR‐31‐5p, and collagen type V alpha 1 chain (COL5A1) in LSCC tissues and cells was detected by quantitative real‐time polymerase chain reaction. Cell counting kit 8 and 5‐Ethynyl‐2′‐deoxyuridine assays were used to detect cell proliferation. Wound healing assay and transwell assay were used to test cell migration and invasion, respectively. The protein expression in LSCC cells was detected with western blot. The relationships between miR‐31‐5p and circBFAR or COL5A1 were identified by dual‐luciferase reporter assay, RNA‐pull down assay, and immunoprecipitation assay. The effect of circBFAR on tumor growth in vivo was detected by tumor xenograft mice experiment. The protein expression of COL5A1 and KI‐67 in LSCC tissues was measured by immunohistochemistry assay. Results CircBFAR was increased in LSCC tissues and cells, and was related to advanced clinical stage and overall survival of LSCC patients. The cell viability and proliferation were inhibited by circBFAR knockdown and silencing of circBFAR blocked migration and invasion of LSCC cells. CircBFAR knockdown suppressed cell tube formation, and the protein expression of KI‐67, matrix metallopeptidase 2 (MMP2), and vascular endothelial growth factor A (VEGFA) in LSCC cells. MiR‐31‐5p was the target of circBFAR, and the inhibitory effects of circBFAR deficiency on viability, proliferation, migration, invasion, tube formation and the protein expression of KI‐67, MMP2, and VEGFA in LSCC cells were rescued by miR‐31‐5p downregulation. COL5A1 was negatively regulated by miR‐31‐5p, and was boosted in LSCC tissues and cells. COL5A1 overexpression reversed the inhibitory effects of miR‐31‐5p on LSCC cells. CircBFAR insufficiency hindered tumor growth in vivo. Conclusion CircBFAR, miR‐31‐5p, and COL5A1 in LSCC progression might provide novel therapeutic targets for LSCC clinical intervention.https://doi.org/10.1002/lio2.966CircBFARCOL5A1laryngeal squamous cell cancermiR‐31‐5p |
| spellingShingle | Hengcui Gong Wei Wu Chuankai Fang Di He CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through miR‐31‐5p/COL5A1 axis Laryngoscope Investigative Otolaryngology CircBFAR COL5A1 laryngeal squamous cell cancer miR‐31‐5p |
| title | CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through miR‐31‐5p/COL5A1 axis |
| title_full | CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through miR‐31‐5p/COL5A1 axis |
| title_fullStr | CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through miR‐31‐5p/COL5A1 axis |
| title_full_unstemmed | CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through miR‐31‐5p/COL5A1 axis |
| title_short | CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through miR‐31‐5p/COL5A1 axis |
| title_sort | circbfar correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through mir 31 5p col5a1 axis |
| topic | CircBFAR COL5A1 laryngeal squamous cell cancer miR‐31‐5p |
| url | https://doi.org/10.1002/lio2.966 |
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